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2006 Fiscal Year Final Research Report Summary

Study on adaptation of single photosynthetic cells against environmental stress by using three-dimensional super-resolution microscopy with time resolution ability

Research Project

Project/Area Number 16072209
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKyoto University

Principal Investigator

KUMAZAKI Shigeichi  Kyoto University, Graduate school of science, Associate Professor (40293401)

Co-Investigator(Kenkyū-buntansha) NISHIYAMA Masayoshi  Kyoto University, Graduate school of science, Assistant Professor (10346075)
OH-OKA Hirozo  Osaka University, Graduate school of science, Associate Professor (30201966)
Project Period (FY) 2004 – 2006
KeywordsMicroscopic spectroscopy / fluorescence spectra / confocal microscopy / photosynthesis / chloroplast / thylakoid membrane / cyanobacteria / photosystems
Research Abstract

A near-infrared fs pulse was illuminated on a line in a specimen by a resonant scanning mirror oscillating at 7.9kHz, and total multiphoton-induced fluorescence from the linear region was focused on the slit of an imaging polychromator. An electron-multiplying CCD camera was used to resolve fluorescence of different colors at different horizontal pixels and fluorescence of different spatial positions in a specimen at different vertical pixels. The full widths at half maximum of the point-spread function of the system were estimated to be 0.39-0.40, 0.33 and 0.56-0.59μm for the X, Y and Z axes, respectively, at fluorescence wavelengths between 644 and 690nm. This microscope was applied to a study of the subcellular fluorescence spectra of thylakoid membranes in a cyanobacterium, Anabaena. It was found that the fluorescence intensity ratio between chlorophyll molecules mainly of photosystem II and phycobilin molecules of phycobilisome (chlorophyll/phycobilin), became lower as one probed deeper inside the cells. This was attributable not to position dependence of re-absorption or scattering effects, but to an intrinsic change in the local physiological state of the thylakoid membrane, with the help of a transmission spectral measurement of subcellular domains. Adaptation of the thylakoid membrane against light conditions, and its variation between chloroplasts and cyanobacteria, has been known for decades, but characterization based on fluorescence spectrum at a subcellular resolution has been scarce. Our newly developed spectroimaging system has also been applied to chloroplasts in tabacco, zea mays, and chlorella. Tissue dependence in the case of tobacco, daily cycle in the case of zea mays, and culture-condition dependence in the case of chlorella were found.

  • Research Products

    (43 results)

All 2008 2007 2006 2005 2004

All Journal Article (15 results) (of which Peer Reviewed: 9 results) Presentation (25 results) Book (3 results)

  • [Journal Article] Soluble cytochrome c-554, CycA, is not essential for photosynthetic electron transfer in Chlorobium tepidum2008

    • Author(s)
      Y. Tsukatani, et al.
    • Journal Title

      FEBS Lett (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cloning and Characterization of a Novel Gene Involved in Nitrogen Fixation in Heliobacterium Chlorum : a Possible Regulatory Gene2006

    • Author(s)
      J. Enkh-Amgalan
    • Journal Title

      Arch. Microbiol. 186

      Pages: 327-337

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Crystal Structure of BchU, a Methyltransferase Involved in Bacteriochlorophyll c Biosynthesis, and its Complex with S-adenosylhomocystein : Implications for Reaction Mechanism2006

    • Author(s)
      K. Wada, et al.
    • Journal Title

      J. Mol. Biol. 360

      Pages: 839-849

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] ESR Signal of the Iron-sulfur Center FX and its Function in the Horaodimeric Reaction Center of Heliobacterium Modesticaldum2006

    • Author(s)
      R. Miyamoto, et al.
    • Journal Title

      Biochemistry 45

      Pages: 6306-6316

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Cloning and Characterization of a Novel Gene Involved in Nitrogen Fixation in Heliobacterium Chlorum : a Possible Regulatory Gene2006

    • Author(s)
      J. Enkh-Amgalan, et al.
    • Journal Title

      Arch. Microbiol. 186

      Pages: 327-337

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ESR Signal of the Iron-sulfur Center FX and its Function in the Homodimeric Reaction Center of Heliobacterium Modesticaldum2006

    • Author(s)
      R. Miyamoto, et al.
    • Journal Title

      Biochemistry 45

      Pages: 6306-6316

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Entropy rectifies the Brownian steps of kinesin2005

    • Author(s)
      Y. Taniguchi, et al.
    • Journal Title

      Nature Chemical Biology 1

      Pages: 342-347

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Different sensitivities to oxygen between two strains of the photosynthetic green sulfur bacterium Chlorobium vibrioforme NCIB 8327 with bacteriochlorophyll c and d2005

    • Author(s)
      J. Harada, et al.
    • Journal Title

      Photosynth. Res. 86

      Pages: 137-143

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray diffraction study of methyltransferase BchU involved in bacterioohlorophyll c biosynthesis2005

    • Author(s)
      J. Harada, et al.
    • Journal Title

      Acta Crystallographica F61

      Pages: 712-714

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Determination of stereochemistry of bacteriochlorophyll-g_F and 8^1 -hydroxy-chlorophyll -a_F from Heliobacterium modes ticaldum2005

    • Author(s)
      T. Mizoguchi, et al.
    • Journal Title

      Photochem. Photobiol 8

      Pages: 666-673

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] In vitro activity of C-20 methyltransferase, BchU, involved in bacteriochlorophyll c biosynthetic pathway in green sulfur bacteria2005

    • Author(s)
      J. Harada, et al.
    • Journal Title

      FEBS Lett. 579

      Pages: 1983-1987

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray diffraction study of methyltransferase BchU involved in bacteriochlorophyll c biosynthesis2005

    • Author(s)
      J. Harada, et al.
    • Journal Title

      Acta Crystallographica F61

      Pages: 712-714

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Determination of stereochemistry of bacteriochlorophyll-g_F and 8^1-hydroxy-chlorophyll -a_F from Heliobacterium modesticaldum2005

    • Author(s)
      T. Mizoguchi, et al.
    • Journal Title

      Photochem. Photobiol. 8

      Pages: 666-673

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation: Regulation of energy transfer and ferredoxin-mediated NADP^+ reduction on the cytoplasmic side2004

    • Author(s)
      Y. Tsukatani
    • Journal Title

      J. Biol. Chem. 279

      Pages: 51122-51130

    • Description
      「研究成果報告書概要(和文)」より
    • Peer Reviewed
  • [Journal Article] Function of a PscD subunit in a homodimeric reaction center complex of the photosynthetic green sulfur bacterium Chlorobium tepidum studied by insertional gene inactivation : Regulation of energy transfer and ferredoxin-mediated NADP^+ reduction on the cytoplasmic side2004

    • Author(s)
      Y. Tsukatani, et al.
    • Journal Title

      J. Biol. Chem. 279

      Pages: 51122-51130

    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Detection of quinone function in the homodimeric type-I reaction center of Heliobacterium modesticaldum2007

    • Author(s)
      T. Kondo, et al.
    • Organizer
      14^<th> International Congress of Photosynthesis
    • Place of Presentation
      Glasgow. Scotland
    • Year and Date
      20070723-20070727
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Bifurcated electron donations from quinol oxidoreductase and soluble CycA to cytochrome c_z of the photosynthetic reaction center complex in the green sulfur bacterium Chlorobium2007

    • Author(s)
      C. Azai, et al.
    • Organizer
      14^<th> International Congress of Photosynthesis
    • Place of Presentation
      Glasgow. Scotland
    • Year and Date
      20070723-20070727
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The Heme-containing portion of cytochrome c_z from Chlorobium tepidum : Its over-expression in Escherichia coli and spectroscopic studies2007

    • Author(s)
      H. Oh-oka, et al.
    • Organizer
      14^<th> International Congress of Photosynthesis
    • Place of Presentation
      Glasgow. Scotland
    • Year and Date
      20070723-20070727
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Compositional alternation of bacteriochlorophyll a derivatives and carotenoid species localized in RC-LH1 core complex, LH2 and LH4 of the purple bacterium, Rhodopseudomonas sp. strain Rits, under various light intensity2007

    • Author(s)
      J. Harada, et al.
    • Organizer
      Satellite Meeting : Light-harvesting Systems Workshop (LH2007)
    • Place of Presentation
      Glasgow, Scotland
    • Year and Date
      20070719-20070721
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] The effect of light intensity on composition of photosynthetic pigments in light-harvesting systems of the purple photosynthetic bacterium, Rhodopseudomonas sp. strain Rits2007

    • Author(s)
      J. Harada, et al.
    • Organizer
      The 3rd International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Shiga, Janan
    • Year and Date
      20070611-20070613
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Microscopic Analysis of Kinesin-microtubule Complex under High Hydrostatic Pressure2007

    • Author(s)
      M. Nishiyama
    • Organizer
      51st Annual Meeting of the Biophysical Society
    • Place of Presentation
      Baltimore, USA
    • Year and Date
      20070303-20070307
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] How Does High Pressure Affect on the Bacterial Motility?2006

    • Author(s)
      M. Nishiyama
    • Organizer
      5th East Asian Biophysics Symposium & 44th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Okinawa, Japan
    • Year and Date
      20061112-20061116
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Development of a High-pressure Microscope and its Applicatiion to Biological Systems2006

    • Author(s)
      M. Nishiyama
    • Organizer
      Micro-NanoMechatronics and Human Science, 2006 IEEE International Symposium
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      20061106-20061108
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Development of a High-pressure Microscope and its Applicatiion to Kinesin-microtubule Complex2006

    • Author(s)
      M. Nishiyama
    • Organizer
      2006 Biophysical Discussions "Molecular Motors : Point Counterpoint"
    • Place of Presentation
      Asilomar, USA
    • Year and Date
      20061019-20061022
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Single Molecule Analysis of Stepwise Movement of ATP-driven Molecular Motors2006

    • Author(s)
      M. Nishiyama
    • Organizer
      Tamura Symposium, Frontiers in Dynamics : Physical and Biological Systems
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20060521-20060524
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Methylation at the meso-position of chlorophylls by C20- methyltransferase of photosynthetic green sulfur bacteria2005

    • Author(s)
      H. Tamiaki
    • Organizer
      Pacichem2005
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      20051215-20051220
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Methylation at the meso-position of chlorophylls by C20-methyltransferase of photosynthetic green sulfur bacteria2005

    • Author(s)
      H. Tamiaki, et al.
    • Organizer
      Pacichem2005
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      20051215-20051220
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Structural basis for the methylation mechanism in methyltransferase BchU involved in bacteriochlorophyll c biosynthesis2005

    • Author(s)
      K. Wada
    • Organizer
      XX Congress of the International Union of Crystallography
    • Place of Presentation
      Florence, Italy
    • Year and Date
      20050823-20050831
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] A key enzyme of biosynthesis between bacteriochlorophyll c and d, BchU, derived from the photosynthetic green sulfur bacterium Chlorobium tepidum2005

    • Author(s)
      J. Harada
    • Organizer
      Second International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Shiga, Japan
    • Year and Date
      20050616-20050618
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Stepping kinetics of kinesin motors is described by load-dependent Michaelis constant2005

    • Author(s)
      M. Nishiyama
    • Organizer
      Biophysical Society 49th Annual meeting
    • Place of Presentation
      Long Beach、USA
    • Year and Date
      20050212-20050216
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Stepping kinetics of kinesin motors is described by load-dependent Michaelis constant2005

    • Author(s)
      M. Nishiyama, et al.
    • Organizer
      Biophysical Society 49th Annual meeting
    • Place of Presentation
      Long Beach、 USA
    • Year and Date
      20050212-20050216
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] Energy transfer and charge separation dynamics in chlorophylld-based Photosystem I from Acaryochloris marina2004

    • Author(s)
      S. Kumazaki
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20040829-20040903
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The function of the PscD subunit of the photosynthetic reaction center complex in Chlorobium tepidum2004

    • Author(s)
      Y. Tsukatani
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20040829-20040903
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Spectroscopic studies of electron transfer reactions in membranes and isolated reaction center from Heliobacterium modesticaldum2004

    • Author(s)
      H. Oh-oka
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20040829-20040903
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Natural reverse mutations of the inactivated bchU gene in Chlorobium vibrioforme NCIB 83272004

    • Author(s)
      J. Harada
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20040829-20040903
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Energy transfer and charge separation dynamics in chlorophyll d-based Photosystem I from Acaryochloris marina2004

    • Author(s)
      S. Kumazaki, et al.
    • Organizer
      13th International Congress on Photosynthesis
    • Place of Presentation
      Montreal, Canada
    • Year and Date
      20040829-20040903
    • Description
      「研究成果報告書概要(欧文)」より
  • [Presentation] In Vitro Enzymatic Activity of C-20 Methyltransferase, BchU, in the Bacteriochlorophyll c Biosynthetic Pathway2004

    • Author(s)
      J. Harada
    • Organizer
      13th International Congress on Photosynthesis Satellite Workshop : Photosynthetic Light-Harvesting Systems
    • Place of Presentation
      Sainte-Adele, Canada
    • Year and Date
      20040826-20040829
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Enzymatic activity of C-20 methyltransferase, BchU, involved in biosynthesis of bacteriochlorophyll c2004

    • Author(s)
      J. Harada
    • Organizer
      International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Kusatsu, Japan
    • Year and Date
      20040616-20040618
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] The electron transfer pathway in heliobacterial reaction center : Reinvestigation by a combination of the spectroscopic methods and 3-D structural modeling2004

    • Author(s)
      H. Oh-oka
    • Organizer
      International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Kusatsu, Japan
    • Year and Date
      20040616-20040618
    • Description
      「研究成果報告書概要(和文)」より
  • [Presentation] Elucidation of stereochemistry of bacteriochlorophyll-g_F and 8^1-hydroxy-chlorophyll-a_F from Heliobacterium modesticaldum2004

    • Author(s)
      T. Mizoguchi
    • Organizer
      International Workshop on Supremolecular Nanoscience of Chemically Programmed Pigments
    • Place of Presentation
      Kusatsu, Japan
    • Year and Date
      20040616-20040618
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Energy from the Sun (分担執筆)2008

    • Author(s)
      S. Kumazaki, et al.
    • Total Pages
      4
    • Publisher
      Springer
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Molecular Nono Dynamics (分担執筆)2008

    • Author(s)
      S. Kumazaki, et al.
    • Total Pages
      20
    • Publisher
      Wiley-VCH
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] フェムト秒テクノロジー(分担執筆)2006

    • Author(s)
      熊崎茂一
    • Total Pages
      8
    • Publisher
      化学同人
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2010-06-09  

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