2006 Fiscal Year Final Research Report Summary
Creation of Reaction Fields in Single Bio Cells and Measurement of Their Reactions
| Project/Area Number |
16072211
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Science and Engineering
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| Research Institution | Osaka University |
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Principal Investigator |
MASUHARA Hiroshi Osaka University, Graduate School of Engineering, Professor (60029551)
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| Co-Investigator(Kenkyū-buntansha) |
ASAHI Tsuyoshi Osaka University, Graduate School of Engineering, Assosiate Professor (20243165)
HIRAKI Yuji Kyoto University, Institute of Frontier Medical Science, Assosiate Professor (40144498)
IMAMOTO Yasushi Kyoto University, Graduate School of Science, Assosiate Professor (80263200)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Light Scattering Microspectroscopy / Single Bio Cell / Femtosecond Laser / Mousr NIH3T3 Cell / Dextran / Fluorescence Imaging / Actin Stress Fiber / EGFP Modification |
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| Research Abstract |
1. Development of Rayleigh light scattering spectroscopy/imaging system and its application to single bio cells
Single mouse NIH3T3 cells incubated before and after a PBS buffer containing gold nanoparticles were measured by a newly developed system with a dark field microscope. The scattering images were obtained by scanning a focused white light, and its spectrum at each point was acquired. The nanoparticles are sometimes aggregated with each other and distributed inhomogeneously and on the living cells. Nanoparticle spectra were also examined in detail.
2. Femtosecond laser injection of nanoparticles into single bio cells and their expression of injected DNA
The laser pulse was focused outside of mouse NIH3T3 single cells and the polymer nanoparticles on the cell were introduced into them. The nanosparticles distribution inside the cell is local and spatially controllable. DNA plasmid of enhanced green fluorescent protein (EGFP) and its model compound (FITC dextram) was injected by direct irradiation of cell and nucleus membranes. The expression of the DNA was successful and their conditions were examined.
3. Structural changes of bio cells induced by femtosecond laser irradiation and their fluorescence spectral imaging
Mouse NIH3T3 cells whose actins are bound with EGFP were imaged and irradiated by femtosecond laser pulse. The dynamics of the resultant damages and recovery process of actin stress fiber, cell membrane, filopodia and lamellipodia were followed by fluorescence imaging.
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[Journal Article] Dual Effects of the Membrane-anchored MMP Regulator RECK on Chondrogenic Differentiation of ATDC5 Cells2007
Author(s)
Shunya Kondo, Chisa Shukunami, Yoko Morioka, Naoya Matsumoto, Rei Takahashi, Junseo Oh, Tadao Atsumi, Akihiro Umezawa, Akira Kudo, Hitoshi Kitayama, Yuji Hiraki, Makoto Noda
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Journal Title
J. Cell Sci. 120
Pages: 849-857
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Chondromodulin-I Maintains Cardiac Valvular Function by Preventing Angiogenesis2006
Author(s)
Masatoyo Yoshioka, Shinsuke Yuasa, Keisuke Matsumura, Takayuki Shiomi, Chisa Shukunami, Yasunori Okada, Makio Mukai, Hankei Shin, Ryohei Yozu, Masataka Sata, Satoshi Ogawa, Yuji Hiraki, Keiichi Fukuda
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Journal Title
Nature Medicine 12(10)
Pages: 1151-1159
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Organic Nonlinear Optical DAST Crystals for Electro-Optic Measurement and Terahertz Wave Generation2006
Author(s)
Yoshinori Takahashi, Hiroaki Adachi, Tetsuo Taniuchi, Masayuki Takagi, Youichiroh Hosokawa, Shinji Onzuka, Srinivasan Brahadeeswaran, Masashi Yoshimura, Yusuke Mori, Hiroshi Masuhara, Takatomo Sasaki, Hachiro Nakanishi
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Journal Title
J. Photochem. Photobiol. A: Chem. 183 (3)
Pages: 247-252
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays2006
Author(s)
Keiko Ikeda, Hiroshi Nakazawa, Ai Shimo-Oka, Kaoru Ishio, Seiji Miyata, Yoichiroh Hosokawa, Satoshi Matsumura, Hiroshi Masuhara, Serge Belloncik, Robert Alain, Naoki Goshima, Nobuo Nomura, Kenichi Morigaki, Atsushi Kawai, Toshihiro Kuroita, Bunsei Kawakami, Yaeta Endo, Hajime Mori
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Journal Title
Proteomics 6(1)
Pages: 54-66
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Chondromodulin-I Maintains Cardiac Valvular Function by Preventing Angiogenesis2006
Author(s)
Masatoyo Yoshioka, Shinsuke Yuasa, Keisuke Matsumura, Takayuki Shiomi, Chisa Shukunami, Yasunori Okada, Makio Mukai, Hankei Shin, Ryohei Yozu, Masataka Sata, Satoshi Ogawa, Yuji Hiraki, Keiichi Fukuda
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Journal Title
Nature Medicine 12 (10)
Pages: 1151-1159
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Organic Nonlinear Optical DAST Crystals for Electro-optic Measurement and Terahertz Wave Generation2006
Author(s)
Yoshinori Takahashi, Hiroaki Adachi, Tetsuo Taniuchi, Masayuki Takagi, Youichiroh Hosokawa, Shinji Onzuka, Srinivasan Brahadeeswaran, Masashi Yoshimura, Yusuke Mori, Hiroshi Masuhara, Takatomo Sasaki, Hachiro Nakanishi
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Journal Title
J. Photochem. Photobiol. A: Chem. 183 (3)
Pages: 247-252
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Immobilization of diverse foreign proteins in viral polyhedra and potential application for protein microarrays2006
Author(s)
Keiko Ikeda, Hiroshi Nakazawa, Ai Shimo-Oka, Kaoru Ishio, Seiji Miyata, Yoichiroh Hosokawa, Satoshi Matsumura, Hiroshi Masuhara, Serge Belloncik, Robert Alain, Naoki Goshima, Nobuo Nomura, Kenichi Morigaki, Atsushi Kawai, Toshihiro Kuroita, Bunsei Kawakami, Yaeta Endo, Hajime Mori
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Journal Title
Proteomics 6 (1)
Pages: 54-66
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Design, Synthesis, Structural and Nonlinear optical Properties of Photochromic Crystals: Towards Reversible Molecular Switches2005
Author(s)
Michel Sliwa, Sylvie Letard, Isabelle Malfant, Martine Nierlich, Pascal G. Lacroix, Tsuyoshi Asahi, Hiroshi Masuhara, Pei Yu, Keitaro Nakatani
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Journal Title
Chem. Mater. 17(18)
Pages: 4727-4735
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Design, Synthesis, Structural and Nonlinear Optical Properties of Photochromic Crystals : Towards Reversible Molecular Switches2005
Author(s)
Michel Sliwa, Sylvie Letard, Isabelle Malfant, Martine Nierlich, Pascal G Lacroix, Tsuyoshi Asahi, Hiroshi Masuhara, Pei Yu, Keitaro Nakatani
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Journal Title
Chem. Mater. 17 (18)
Pages: 4727-4735
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Conformational Change of β5-β6 Loop during the Photocycle of Photoactive Yellow Protein2006
Author(s)
Imamoto, Y., Sasayama, H., Harigai, M., Yamazaki, Y., Kamikubo, H., Kataoka, M.
Organizer
5th East Asian Biophysics Symposium
Place of Presentation
Okinawa, Japan
Year and Date
20061115-20061116
Description
「研究成果報告書概要(和文)」より
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[Presentation] Conformational Change of β5-β6 Loop during the Photocycle of Photoactive Yellow Protein2006
Author(s)
Imamoto, Y., Sasayama, H., Harigai, M., Yamazaki, Y., Kamikubo, H., Kataoka, M.
Organizer
5th East Asian Biophysics Symposium
Place of Presentation
Oinawa, Japan
Year and Date
20061115-20061116
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Femtosecond Laser-induced Cleaving of Protein Crystal in Water Solution2006
Author(s)
Hiroshi Yoshikawa, Masafumi Kashii, Ryota Murai, Yoichiroh Hosokawa, Hiroshi Masuhara, Hiroaki Adachi, Tsuyoshi Inoue, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Takatomo Sasaki
Organizer
5th International Conference on Photo-Excited Processes and Applications
Place of Presentation
Charlottesville, Virginia USA
Year and Date
20060903-20060907
Description
「研究成果報告書概要(和文)」より
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[Presentation] Femtosecond Laser-induced Cleaving of Protein Crystal in Water Solution2006
Author(s)
Hiroshi Yoshikawa, Masafumi Kashii, Ryota Murai, Yoichiroh Hosokawa, Hiroshi Masuhara, Hiroaki Adachi, Tsuyoshi Inoue, Hiroyoshi Matsumura, Kazufumi Takano, Satoshi Murakami, Yusuke Mori, Takatomo Sasaki
Organizer
5th International Conference on Photo-Excited Processes and Applications
Place of Presentation
Charlottesville, Virginia, USA
Year and Date
20060903-20060907
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Protein Crystallization at Low Supersaturation by Focused Femtosecond Laser Irradiation2006
Author(s)
Hiroshi Y. Yoshikawa, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Kazufumi Takano, Satoshi Murakami, Yoichiroh Hosokawa, Hiroshi Masuhara, Yusuke Mori, Takatomo Sasaki
Organizer
3rd International Nanophotonics Sympojium Handai (INPS2006)
Place of Presentation
Osaka, Japan
Year and Date
20060705-20060708
Description
「研究成果報告書概要(和文)」より
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[Presentation] Protein Crystallization at Low Supersaturation by Focused Femtosecond Laser Irradiation2006
Author(s)
Hiroshi Y. Yoshikawa, Hiroaki Adachi, Hiroyoshi Matsumura, Tsuyoshi Inoue, Kazufumi Takano, Satoshi Murakami, Yoichiroh Hosokawa, Hiroshi Masuhara, Yusuke Mori, Takatomo Sasaki
Organizer
3rd International Nanophotonics Sympojium Handai (INPS 2006)
Place of Presentation
Osaka, Japan
Year and Date
20060705-20060708
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Secondary Structural Change of Photoactive Yellow Protein2004
Author(s)
Imamoto, Y., Harigai, M., Kamikubo, H., Yamazaki, Y., Kataoka, M.
Organizer
11th International Conference on Retinal Proteins
Place of Presentation
Frauenchiemsee, Germany
Year and Date
20040620-20040624
Description
「研究成果報告書概要(和文)」より
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[Book] 光化学の驚異2006
Author(s)
増原宏, 伊都将司(編著)
Total Pages
232
Publisher
講談社
Description
「研究成果報告書概要(和文)」より
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