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2009 Fiscal Year Final Research Report

X-ray Crystallographic Studies on Mechanism of Photosystem II Membrane Protein Complex

Research Project

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Project/Area Number 16087102
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionOsaka City University (2005-2009)
The Institute of Physical and Chemical Research (2004)

Principal Investigator

KAMIYA Nobuo  Osaka City University, 大学院・理学研究科, 教授 (60152865)

Co-Investigator(Kenkyū-buntansha) SHIN Kenjin  岡山大学, 理学部, 教授 (60261161)
IKEUCHI Akihiko  東京大学, 大学院・総合文化研究科, 教授 (20159601)
NAKAZATO Katsuyoshi  日本大学, 文理学部, 准教授 (50172292)
Co-Investigator(Renkei-kenkyūsha) UMENA Yasufumi  大阪市立大学, 大学院・理学研究科, 博士研究員 (10468267)
Project Period (FY) 2004 – 2009
Keywords光合成 / X線結晶構造解析 / 膜蛋白質 / 超分子 / 光エネルギー変換 / 酸素発生
Research Abstract

Photosystem II (PSII) catalyzes the oxygen evolution from water in the photosynthesis utilizing solar light energy. We succeeded to determine, first in the world, the three-dimensional structure of the oxygen-evolving Mn4Ca cluster at atomic resolution, including the whole structure of PSII. Because PSII also provides chemical energy for CO2 condensation, our results serve structural strict-bases for efficient use of solar energy in the green chemistry.

  • Research Products

    (44 results)

All 2010 2009 2008 2007 2006 2005 2004 Other

All Journal Article (22 results) (of which Peer Reviewed: 21 results) Presentation (18 results) Book (2 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Structural and functional studies on Ycf12 (Psb30) and PsbZ deletion mutants from a thermophilic cyanobacterium2010

    • Author(s)
      Takasaka, K., Iwai, M., Umena, Y., Kawakami, K., Ikeuchi, M., Kamiya, N., Shen, J. -R.
    • Journal Title

      BBA 1797

      Pages: 278-284

    • Peer Reviewed
  • [Journal Article] Location of chloride and its possible functions in oxygen-evolving photosystem II revealed by X-ray crystallography2009

    • Author(s)
      Kawakami, K., Umena, Y., Kamiya, N., Shen, J. -R.
    • Journal Title

      PNAS 106

      Pages: 8567-8572

    • Peer Reviewed
  • [Journal Article] Towards structural elucidation of eukaryotic photosystem II: Purification, crystallization and preliminary X-ray diffraction analysis of photosystem II from a red alga2009

    • Author(s)
      Adachi, H., Umena, Y., Enami, I., Henmi, T., Kamiya, N., Shen, J. -R.
    • Journal Title

      BBA 1787

      Pages: 121-128

    • Peer Reviewed
  • [Journal Article] Is the photosystem II complex a monomer or a dimer?2009

    • Author(s)
      Watanabe, M., Iwai, M., Narikawa, R., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 1674-1680

    • Peer Reviewed
  • [Journal Article] X-ray crystallographic and biochemical characterizations of a mutant Photosystem II complex from Thermosynechococcus vulcanus with the psbTc gene inactivated by an insertion mutation2008

    • Author(s)
      T. Henmi, M. Iwai, M. Ikeuchi, K. Kawakami, J. -R. Shen, N. Kamiya
    • Journal Title

      J. Synchrotron Rad. 15

      Pages: 304-307

    • Peer Reviewed
  • [Journal Article] 2, 2 ' -b-hydroxylase is involved in carotenogenesis of both nostoxanthin and 2-hydroxymyxol 2 ' -fucoside in Thermosynechococcus elongatus strain BP-1.2008

    • Author(s)
      Iwai, M., Maoka, T., Ikeuchi, M., Takaichi, S.
    • Journal Title

      Plant Cell Physiol. 49

      Pages: 1678-1687

    • Peer Reviewed
  • [Journal Article] Crystal structural analysis of photosystem II complex with the novel method to reduce X-ray radiation damage2008

    • Author(s)
      A. Okumura, R. Nagao, T. Suzuki, S. Yamagoe, M. Iwai, K. Nakazato, I. Enami
    • Journal Title

      BBA 1777

      Pages: 1545-1551

    • Peer Reviewed
  • [Journal Article] Location of PsbY in oxygen-evolving photosystem II revealed by mutagenesis and X-ray crystallography2007

    • Author(s)
      Keisuke Kawakami, Masako Iwai, Masahiko Ikeuchi, Nobuo Kamiya, Jian-Ren Shen
    • Journal Title

      FEBS Lett. 581

      Pages: 4983-4987

    • Peer Reviewed
  • [Journal Article] Aromatic structure of Tyrosine-92 in the extrinsic PsbU protein of red algal Photosystem II is important for its functioning2007

    • Author(s)
      Akinori Okumura, Masanori Sano, Takehiro Suzuki, Hiroyasu Tanaka, Ryo Nagao, Katsuyoshi Nakazato, Masako Iwai, Hideyuki Adachi, Jian-Ren Shen, Isao Enami
    • Journal Title

      FEBS Lett. 581

      Pages: 5255-5258

    • Peer Reviewed
  • [Journal Article] g-Anisotropy of the S2-state manganese cluster in single crystals of cyanobacterial photosystem II studied by W-band electron paramagnetic resonance spectroscopy2006

    • Author(s)
      Matsuoka H., Furukawa K., Kato T., Mino H., Shen J. -R., Kawamori A.
    • Journal Title

      J. Phys. Chem. B. 110

      Pages: 13242-13247

    • Peer Reviewed
  • [Journal Article] Lipids in Oxygen-Evolving Photosystem II Complexes of Cyanobacteria and Higher Plants2006

    • Author(s)
      Sakurai I., Shen J. -R., Leng J., Ohashi S., Kobayashi M., Wada H.
    • Journal Title

      J. Biochem. (Tokyo) 140

      Pages: 201-209

    • Peer Reviewed
  • [Journal Article] Absence of the psbH gene product destabilizes Photosystem II complex: the lack of PSII-X protein in the thermophilic cyanobacterium Thermosynechococcus elongatus BP-12006

    • Author(s)
      Iwai, M., Katayama, M., Ikeuchi, M.
    • Journal Title

      Photosynth. Res. 87

      Pages: 313-322

    • Peer Reviewed
  • [Journal Article] Mutations in a putative chloride efflux transporter gene suppress the chloride requirement of the photosystem II in pdbV-disruptant (or cytochrome c550 deficient mutant)2006

    • Author(s)
      Kobayashi, M., Katoh, H., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 47

      Pages: 799-804

    • Peer Reviewed
  • [Journal Article] Pressure Equilibrium and Jump Study on Unfolding of 23kDa Protein from Spinach Photosystem II2005

    • Author(s)
      Tan C. -Y., Xu C. -H., Wong J., Shen J. -R., Sakuma S., Yamamoto Y., Lange R., Balny C., Ruan K. -C.
    • Journal Title

      Biophys J. 88

      Pages: 1264-1275

    • Peer Reviewed
  • [Journal Article] Key cofactors of photosystem II cores from four organisms identified by 1. 7-K absorption2005

    • Author(s)
      Arskold S.P., Smith P.J., Shen J. -R., Pace R.J., Krausz E.
    • Journal Title

      CD and MCD, Photosynthesis Res. 84

      Pages: 309-316

    • Peer Reviewed
  • [Journal Article] Distribution of the extrinsic proteins as a potential marker for the evolution of photosynthetic oxygen-evolving photosystem II2005

    • Author(s)
      Enami I., Suzuki T., Tada O., Nakada Y., Nakamura K., Tohri A., Ohta H., Inoue I., Shen J. -R.
    • Journal Title

      FEBS Journal 272

      Pages: 983-990

    • Peer Reviewed
  • [Journal Article] Quality control of photosystem II: An FtsH protease plays an essential role in the turnover of the reaction center D1 protein in Synechocystis PCC 6803 under heat stress as well as light stress conditions2005

    • Author(s)
      Kamata T., Hiramoto H., Morita N., Shen J. -R., Mann N.H., Yamamoto Y.
    • Journal Title

      Photochemical & Photobiological Sciences 4

      Pages: 83-990

    • Peer Reviewed
  • [Journal Article] Pigment exchange of Phosystem II reaction center by chlorophyll d2005

    • Author(s)
      Tomo T., Hirano E., Nagata J., Nakazato K.
    • Journal Title

      Photosynthesis Research 84

      Pages: 77-83

    • Peer Reviewed
  • [Journal Article] 光化学系II複合体の構造と機能2005

    • Author(s)
      神谷信夫, 沈建仁
    • Journal Title

      蛋白質核酸酵素 50

      Pages: 1174-1179

  • [Journal Article] Improved genetic transformation of the thermophilic cyanobacterium2004

    • Author(s)
      Iwai, M., Katoh, H., Katayama, M., Ikeuchi, M.
    • Journal Title

      Thermosynechococcus elongatus BP-1, Plant Cell Physiol. 45

      Pages: 171-175

    • Peer Reviewed
  • [Journal Article] Response to oxidative stress involves a novel peroxiredoxin gene in the unicellular cyanobacterium Synechocystis sp. PCC 68032004

    • Author(s)
      Kobayashi, M., Ishizuka, T., Katayama, M., Kanehisa, M., Bhattacharyya-Pakrasi, M., Pakrasi, H.B., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 50

      Pages: 290-299

    • Peer Reviewed
  • [Journal Article] PSII-Tc protein plays an important role in dimerization of Photosystem II.2004

    • Author(s)
      Iwai, M., Katoh, H., Katayama, M., Ikeuchi, M.
    • Journal Title

      Plant Cell Physiol. 45

      Pages: 1809-1816

    • Peer Reviewed
  • [Presentation] 光化学系II複合体結晶の分解能向上2010

    • Author(s)
      川上恵典、梅名泰史、田代隆慶、神谷信夫、沈建仁
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-20100321
  • [Presentation] 紅藻由来光化学系II複合体の精製と結晶の分解能の改善2010

    • Author(s)
      足立秀行、梅名泰史、田代隆慶、榎並勲、神谷信夫、沈建仁
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本
    • Year and Date
      20100318-20100321
  • [Presentation] Structure around Oxygen- Evolving Mn4OxCa-Cluster of Photosystem II Complex from Thermosynechococcus vulcanus2010

    • Author(s)
      N. Kamiya
    • Organizer
      2010 OCU International Symposium on the Foundation of Enbironmental Research
    • Place of Presentation
      Awaji, Japan
    • Year and Date
      20100308-20100309
  • [Presentation] 光化学系II膜蛋白質複合体の酸素発生中心の構造研究2009

    • Author(s)
      梅名泰史, 川上恵典, 大熊章郎, 西条慎也, 内藤久志, 沈建仁, 神谷信夫
    • Organizer
      日本結晶学会
    • Place of Presentation
      大坂
    • Year and Date
      20091205-20091206
  • [Presentation] X線損傷低減データに基づく光化学系II複合体の酸素発生中心の構造研究2009

    • Author(s)
      梅名泰史・川上恵典・大熊章郎・西条慎也・内藤久志・沈建仁・神谷信夫
    • Organizer
      第9回日本蛋白質科学会年会
    • Place of Presentation
      熊本
    • Year and Date
      20090520-20090522
  • [Presentation] 光化学系II二量体の形成・安定化におけるPsbM, PsbIサブユニットの役割2009

    • Author(s)
      川上恵典, 岩井雅子, 川端洋輔, 逸見隆博, 池内昌彦, 神谷信夫, 沈建仁
    • Organizer
      第50回日本植物生理学会年会
    • Year and Date
      20090321-20090324
  • [Presentation] Ycf12、PsbZ欠失変異体由来光化学系IIのX線結晶構造解析2009

    • Author(s)
      高坂賢之、岩井雅子、梅名泰史、神谷信夫、川上恵典、沈建仁
    • Organizer
      第50回日本植物生理学会年会
    • Year and Date
      20090321-20090324
  • [Presentation] X線損傷低減データに基づく酸素発生光化学系II粒子の構造精密化2008

    • Author(s)
      梅名泰史, 川上恵典, 大熊章郎, 西条慎也, 内藤久志, 沈建仁, 神谷信夫
    • Organizer
      日本結晶学会2007年度年会
    • Year and Date
      20081201-20081202
  • [Presentation] Crystal structural analysis of photosystem II complex with the novel method to reduce X-ray radiation damage2008

    • Author(s)
      Y. Umena, K. Kawakami, A. Ohkuma, S. Saijyou, H. Naitow, J. -R. Shen, N. Kamiya
    • Organizer
      XXI Congress of the International Union of Crystallography
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      20080823-20080831
  • [Presentation] Coordination structure and functional implications of two Cl-binding sites in oxygen-evolving photosystem II2008

    • Author(s)
      K. Kawakami, Y. Umena, N. Kamiya, J. -R. Shen
    • Organizer
      XXI Congress of the International Union of Crystallography
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      20080823-20080831
  • [Presentation] Purification, crystallization and preliminary X-ray analysis of photosystem II dimer from a red alga Cyanidium caldarium2008

    • Author(s)
      H. Adachi, Y. Umena, I. Enami, N. Kamiya, J. -R. Shen
    • Organizer
      XXI Congress of the International Union of Crystallography
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      20080823-20080831
  • [Presentation] psbTc遺伝子を破壊した好熱性ラン藻変異株光化学系II複合体の結晶構造解析2008

    • Author(s)
      逸見隆博、岩井雅子、池内昌彦、沈建仁、神谷信夫
    • Organizer
      植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      20080328-20080330
  • [Presentation] 紅藻Cyanidium caldarium由来光化学系II複合体の精製・結晶化2008

    • Author(s)
      足立秀行、榎並勲、逸見隆博、神谷信夫、沈建仁
    • Organizer
      植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      20080328-20080330
  • [Presentation] PsbMサブユニットを欠失した光化学系II複合体の結晶構造解析2008

    • Author(s)
      川端洋輔、逸見隆博、岩井雅子、末益卓、川上恵典、青山智佳、池内昌彦、神谷信夫、沈建仁
    • Organizer
      植物生理学会年会
    • Place of Presentation
      札幌
    • Year and Date
      20080328-20080330
  • [Presentation] Crystal Structure Analysis of a Mutant Photosystem II Complex from Thermosynechococcus vulcanus with the psbTc Gene Inactivated by an Insertion Mutation2007

    • Author(s)
      T. Henmi, M. Iwai, M. Ikeuchi, K. Kawakami, J. -R. Shen, N. Kamiya
    • Organizer
      The second International Symposium on Diffraction Structural Biology 2007
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20070910-20070913
  • [Presentation] Structural analysis of mutant Photosystem II complexes from Thermosynechococcus vulcanus2007

    • Author(s)
      K. Kawakami, Y. Kawabata, T. Henmi, M. Iwai, M. Ikeuchi, N. Kamiya, J. -R. Shen
    • Organizer
      The second International Symposium on Diffraction Structural Biology 2007
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20070910-20070913
  • [Presentation] Purification and crystallization of photosystem II complex from a red alga Cyanidium caldarium2007

    • Author(s)
      H. Adachi, I. Enami, T. Henmi, N. Kamiya, J. -R. Shen
    • Organizer
      The second International Symposium on Diffraction Structural Biology 2007
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20070910-20070913
  • [Presentation] 紅藻Cyanidium caldarium光化学系II結晶分解能の改善とx線による分析

    • Author(s)
      足立秀行、梅名泰史、榎並勲、神谷信夫、沈建仁
    • Organizer
      第50回日本植物生理学会年会
    • Year and Date
      00000321-00000324
  • [Book] A Structural Approach(Photosynthetic Protein Complexes)2008

    • Author(s)
      Shen J. -R., Henmi T., Kamiya N
    • Total Pages
      24
    • Publisher
      WILEY-VCH
  • [Book] The Light-Driven Water:Plastoquinone Oxidoreductase2005

    • Author(s)
      Shen J. -R., Kamiya N.
    • Total Pages
      29
    • Publisher
      Springer
  • [Patent(Industrial Property Rights)] タンパク質溶解度測定方法、結晶製造方法、及びそのための装置2008

    • Inventor(s)
      中里勝芳
    • Industrial Property Rights Holder
      中里勝芳
    • Industrial Property Number
      特許・第4110224号
    • Acquisition Date
      2008-04-18
  • [Patent(Industrial Property Rights)] 非イオン性界面活性剤自動除去装置2004

    • Inventor(s)
      中里勝芳
    • Industrial Property Rights Holder
      中里勝芳
    • Industrial Property Number
      特許・第3565683号
    • Acquisition Date
      2004-06-18

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Published: 2011-06-18   Modified: 2016-04-21  

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