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2008 Fiscal Year Final Research Report

Control of embryogenesis of ascidians by localized mRNAs and embryonic induction

Research Project

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Project/Area Number 16107005
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionOsaka University

Principal Investigator

NISHIDA Hiroki  Osaka University, 大学院・理学研究科, 教授 (60192689)

Co-Investigator(Kenkyū-buntansha) KUMANO Gaku  大阪大学, 大学院・理学研究科, 助教 (80372605)
Project Period (FY) 2004 – 2008
Keywords胚発生 / 脊索動物 / ホヤ / 母性mRNA / 局在RNA / 胚誘導 / 繊維芽細胞成長因子 / 脊索
Research Abstract

ホヤは近年、発生過程の詳細な記述、ゲノムプロジェクトの完了などにより、発生生物学における新たなモデル動物として注目を集めている。本研究ではマボヤの初期発生を司るしくみの理解をさらに押し進めるため、卵内で局在しているmRNAについて詳細かつ網羅的な解析を行った。また、32細胞期あたりから始まる誘導的細胞間相互作用について、シグナルに対する応答能を制御する因子、そして誘導によって引き起こされる非対称細胞分裂の制御メカニズムを解析した。

  • Research Products

    (27 results)

All 2010 2009 2008 2007 2006 2005 2004 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (9 results) Book (4 results)

  • [Journal Article] Wnt5 is required for notochord cell intercalation in the ascidian Halocynthia roretzi.2009

    • Author(s)
      Niwano, T., Takatori, N., Kumano, G., Nishida, H.
    • Journal Title

      Biol. Cell 101

      Pages: 645-659

    • Peer Reviewed
  • [Journal Article] Patterning of an ascidian embryo along the anterior-posterior axis through spatial regulation of competence and induction ability by maternally localized2009

    • Author(s)
      Kumano, G., Nishida, H.
    • Journal Title

      PEM. Dev. Biol. 331

      Pages: 78-88

    • Peer Reviewed
  • [Journal Article] Actin microfilaments guide the polarized transport of nuclear pore complexes and the cytoplasmic dispersal of Vasa mRNA during GVBD in the ascidian Halocynthia roretzi.2009

    • Author(s)
      Prodon, F., Hanawa, K., Nishida, H.
    • Journal Title

      Dev. Biol. 330

      Pages: 377-388

    • Peer Reviewed
  • [Journal Article] Ascidians and the plasticity of the chordate developmental program.2008

    • Author(s)
      Lemaire, P., Smith, W.C., Nishida, H.
    • Journal Title

      Curr. Biol. 18

      Pages: R620-R631

    • Peer Reviewed
  • [Journal Article] Cortical and cytoplasmic flows driven by actin microfilaments polarize the cortical ER-mRNA domain along the a-v axis in ascidian oocytes.2008

    • Author(s)
      Prodon, F., Sardet, C., Nishida, H.
    • Journal Title

      Dev. Biol. 313

      Pages: 682-699

    • Peer Reviewed
  • [Journal Article] Localized PEM mRNA and protein are involved in cleavage plane orientation and unequal cell divisions in ascidians.2007

    • Author(s)
      Negishi, T., Takada, T., Kawai, N., Nishida, H.
    • Journal Title

      Curr. Biol. 17

      Pages: 1014-1025

    • Peer Reviewed
  • [Journal Article] Direct activation by Ets and Zic is required for initial expression of the Brachyury gene in the ascidian notochord.2007

    • Author(s)
      Matsumoto, J., Kumano, G., Nishida, H.
    • Journal Title

      Dev. Biol. 306

      Pages: 870-882

    • Peer Reviewed
  • [Journal Article] Cell fate polarization in ascidian mesenchyme/muscle precursors by directed FGF signaling and role for an additional ectodermal FGF antagonizing signal in notochord/nerve cord precursors2007

    • Author(s)
      Kim, G.J., Kumano, G., Nishida, H.
    • Journal Title

      Development 134

      Pages: 1509-1518

    • Peer Reviewed
  • [Journal Article] Brain induction in ascidian embryos is dependent on juxtaposition of FGF9/16/20-producing and-receiving cells.2007

    • Author(s)
      Miyazaki, Y., Nishida, H., Kumano, G.
    • Journal Title

      Dev. Genes Evol. 217

      Pages: 177-188

    • Peer Reviewed
  • [Journal Article] Overlapping expression of FoxA and Zic confers responsiveness to FGF signaling to specify notochord in ascidian embryos.2006

    • Author(s)
      Kumano, G., Yamaguchi, S., Nishida, H.
    • Journal Title

      Dev. Biol. 300

      Pages: 770-784

    • Peer Reviewed
  • [Journal Article] The functional analysis of Type I postplasmic/PEM mRNAs in embryos of the ascidian Halocynthia roretzi.2006

    • Author(s)
      Nakamura, Y., Makabe, K.W., Nishida, H.
    • Journal Title

      Dev. Genes Evol. 216

      Pages: 69-80

    • Peer Reviewed
  • [Journal Article] POPK-1/Sad-1 kinase is required for the proper translocation of maternal mRNAs and putative germ plasm at the posterior pole of the ascidian embryo2005

    • Author(s)
      Nakamura, Y., Makabe, K.W., Nishida, H.
    • Journal Title

      Development 132

      Pages: 4731-4742

    • Peer Reviewed
  • [Journal Article] Tracing cell fate in brain formation during embryogenesis of the ascidian Halocynthia roretzi.2004

    • Author(s)
      Taniguchi, K., Nishida, H.
    • Journal Title

      Dev. Growth Differ. 46

      Pages: 163-180

    • Peer Reviewed
  • [Journal Article] Ets-mediated brain induction in embryos of the ascidian2004

    • Author(s)
      Akanuma, T., Nishida. H.
    • Journal Title

      Halocynthia roretzi. Dev. Genes Evol. 214

      Pages: 1-9

    • Peer Reviewed
  • [Presentation] How do embryos count the cell division rounds in each tissue lineage?: An ascidian case2010

    • Author(s)
      Hiroki Nishida
    • Organizer
      国際シンポジウム
    • Place of Presentation
      Cell cycle and development(京都)
    • Year and Date
      2010-03-15
  • [Presentation] Establishment of embryonic axes and regionalization along the axes: Polarity in organisms and cells.2009

    • Author(s)
      Hiroki Nishida
    • Organizer
      5th Interantional Tunicate Meeting
    • Year and Date
      20090621-20090625
  • [Presentation] Polarization of embryonic cells by prelocalized factors and external signals in ascidians.2009

    • Author(s)
      Hiroki Nishida
    • Organizer
      The CDB Symposium 2009
    • Place of Presentation
      "Shape and Polarity"(神戸)
    • Year and Date
      20090323-20090325
  • [Presentation] ホヤ胚発生における胚と細胞の極性化2008

    • Author(s)
      西田宏記
    • Organizer
      国際高等研究所セミナー
    • Place of Presentation
      奈良
    • Year and Date
      2008-12-06
  • [Presentation] Cleavage pattern control and germ cell specification by localized maternal mRNA in ascidian embryos2006

    • Author(s)
      Hiroki Nishida
    • Organizer
      Swiss-Japan Joint Meeting
    • Place of Presentation
      Swiss, Arosa
    • Year and Date
      20060426-20060426
  • [Presentation] Notochord formation in ascidian embryos2006

    • Author(s)
      Hiroki Nishida
    • Organizer
      1st Meeting Italy-Japan on "VERTEBRATE ORGANOGENESIS"
    • Place of Presentation
      Itary
    • Year and Date
      20060424-20060426
  • [Presentation] Role of localized maternal mRNAs in eggs of a basal chordate, the ascidian Halocynthia roretzi.2005

    • Author(s)
      Hiroki Nishida
    • Organizer
      15th International Congress of Developmental Biology
    • Place of Presentation
      Sidney
    • Year and Date
      20050937
  • [Presentation] Culture, cleavage pattern, and gastrulation of Oikopleura dioica2005

    • Author(s)
      Hiroki Nishida
    • Organizer
      3rd International Tunicate Meeting
    • Place of Presentation
      Santa Barbara
    • Year and Date
      20050709-20050713
  • [Presentation] Role of localized maternal mRNAs in eggs of a basal chordate, the ascidian Halocynthia roretzi2005

    • Author(s)
      Hiroki Nishida
    • Organizer
      International Congress of Experimental Biology
    • Place of Presentation
      San Diego
    • Year and Date
      20050401-20050406
  • [Book] 細胞周期フロンティア2010

    • Author(s)
      西田宏記
    • Publisher
      共立出版(印刷中)
  • [Book] 研究をささえるモデル生物2009

    • Author(s)
      西田宏記
    • Total Pages
      262104-106
    • Publisher
      化学同人
  • [Book] 神経系の多様性:その起源と進化2007

    • Author(s)
      西田宏記
    • Total Pages
      248133-173
    • Publisher
      培風館
  • [Book]

    • Author(s)
      http://www. bio. sci. osaka-u. ac. jp/bio_web/ lab_page/nishida/index. html

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Published: 2011-06-18   Modified: 2016-04-21  

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