2006 Fiscal Year Final Research Report Summary
Serum DNase I activity can be used as a sensitive marker for detection of acute myocardial infarction
| Project/Area Number |
16209023
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Gunma University |
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Principal Investigator |
KOMINATO Yoshihiko Gunma University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (30205512)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Tamiko Gunma University, Gradual School of Medicine, Assistant, 大学院・医学系研究科, 助手 (40008561)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Deoxyribonuclease I / QGP-1 cell / Hypoxia / Transcription / Myocardial infarction / Promoter |
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| Research Abstract |
We demonstrated that serum DNase I activity could be used as novel diagnostic marker for the early detection of acute myocardial infarction (AMI) ; abrupt elevation of serum DNase I activity levels occurs within 3h of the onset of symptoms in patients with AMI, permitting the diagnosis of AMI before accurate CK-MB and c-TnT results become available. Moreover, we investigated alterations in serum DNase I levels after transient ischemia induced during percutaneous coronary intervention(PCI). Serum DNase I activity had risen significantly from base line by 3h after completion of the PCI procedure. However, the mechanism for the elevation of serum DNase I activity induced by ischemia during AMI or PCI remain to be elucidated. To elucidate the molecular basis of this phenomenon, it is important to understand the regulatory mechanism of the human DNase I gene(DNASE1) expression. We first mapped the transcription start sites of DNASE1 in human pancreas and in the DNase I producing human pancr
… More
eatic cancer cell line QGP-1, and revealed a novel site 12kb upstream of exon 1, which was previously believed to be the single transcription starting exon. This initiation site markes an alternative starting exon, designated 1a. Exon 1 and 1a were used simultaneously as transcription starting exon in pancreas and QGP-1 cells. Promoter assay EMSA and chromatin immunoprecipitation analysis with QGP-1 cell lines showed that the promoter region of exon 1a in which the Sp1 transcription factor is specifically involved in promoter activity. This is the first to be identified as a transcription factor responsible for gene expression of vertebrate DNase I gene. Furthermore, RT-PCR analysis indicated alternative splicing of human DNase 1 pre-mRNA in pancreas and QGP-1 cells. Only two transcripts among eight alternative splicing products identified can be translated to produce intact DNase I protein. These results suggested that human DNase 1 expression is regulated through the use of alternative promoter and alternative splicing. Less
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Research Products
(62 results)
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[Journal Article] Association of Gln222Arg polymorphism in the deoxyribonuclease I (DNase I) gene with myocardial infarction in Japanese patients.2006
Author(s)
T.Kumamoto, Y.Kawai, K.Arakawa, N.Morikawa, H.Tada, K.Taniguchi, R.Tatami, I.Miyamori, Y.Kominato, K.Kishi, T.Yasuda.
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Journal Title
European Heart Journal 27(17)
Pages: 2081-2087
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Serum deoxyribonuclease I activity can be used as a sensitive marker for detection of transient myocardial ischemia induced by percutaneous coronary intervention.2005
Author(s)
K.Arakawa, K.Kawai, T.Kumamoto, N.Morikawa, M.Yoshida, H.Tada, R.Kawaguchi, K.Taniguchi, I.Miyamori, Y.Kominato, K.Kishi, T.Yasuda.
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Journal Title
European Heart Journal 26(22)
Pages: 2375-2380
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Improvement of a multiplex PCR system for DYS441, DYS442, DYS443, DYS444 and DYS445, and a population study in 340 Japanese males.2005
Author(s)
Y.Ogata, T.Yamaba, K.awazaki, R.Iida, E.Tsuobota, H.Takatsuka, T.Matsuki, T.Yasuda, K.Kishi.
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Journal Title
Legal Medicine 7(3)
Pages: 183-189
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Forensic application of a multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, DYS445.2005
Author(s)
Y.Ogata, T.Yamaba, K.Sawazaki, R.Iida, H.Takatsuka, M.Masuyama, T.Matsuki, T.Yasuda, K.Kishi
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Journal Title
DNA Polymorphism 13
Pages: 204-206
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The diagnostic use of serum deoxyribonuclease I activity as a novel early-phase marker in acute myocardial infarction.2004
Author(s)
Kawai, M.Yoshida, K.Arakawa, T.Kumamoto, N.Morikawa, N.Tanaka, K.Masamura, H.Tada, S.Ito, H.Hoshizaki, S.Oshima, K.Taniguchi, H.Terasawa, I.Miyamori, K.Kishi, T.Yasuda.
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Journal Title
Circulation 109(20)
Pages: 2398-2400
Description
「研究成果報告書概要(欧文)」より
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