Research for radio-sensitizers which specifically attack tumor cells

2007 Fiscal Year Final Research Report Summary

• Project/Area Number: 16209036
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: National Institute of Radiological Sciences
• Principal Investigator: RYUICHI Okayasu National Institute of Radiological Sciences, Heavy-Ion Radiobiology Research Group, Group Leader (50356135)
• Co-Investigator(Kenkyū-buntansha): YAMADA Shigeru National Institute of Radiological Sciences, Charged Particle Therapy Hospital, Chief Doctor (80311380)
• Project Period (FY): 2004 – 2007
• Keywords: Radio-sensitization / Hsp90 inhibitor / 17AAG / DNA DSB repair / Homologous recombination repair / BRCA2 / siRNA / Rad51

Research Abstract

We performed two specific aims on this project. Results for the individual aim are described.
Aim 1) lb investigate Hsp90 inhibitors as effective radio-sensitizers which have much higher affinity for tumor cells than for normal cells. We showed that 17-AAG effectively radio-sensitized human tumor cells in vitro and in vivo, while normal cells were not affected by the drug. The cause of this sensitization was found out to be inhibition of DNA double strand break (DSB) repair by 17AAG. We also discovered that 17AAG affected the homologous recombination repair (HRR) pathway of DSB repair. Furthermore, the proteins influenced by this chemical were found out to be BRCA2 and Rad51. (Noguchi, et. al. 2006)
Aim 2) lb find an efficient strategy to specifically damage tumor cells, the inhibition of HRR repair mechanism was pursued; the HRR mechanism is known to be active only at late-S and G2 phases of the cell cyde. We used RNA interference technology to knockdown the expression of BRCA2 protein, a crucial molecule for HRR pathway. After efficiently knocking down the protein expression by transfecting tumor cells by BRCA2 small interference RNA (siRNA), significant radio-sensitization was observed in HeLa cells. The cause of this sensitization was found out to be DNA DSB repair inhibition and the HRR pathway was again affected by BRCA2 siRNA transfection in tumor cells. The radio-sensitization was also observed in animal zenograft model. (Yu, et. al. 2008)

Research Products

• [Journal Article] Comparison of the induction and disappearance of DNA double strand breaks and gamma-H2AX foci after irradiation of chromosome in G1-phase or in condensed metaphase cells (2008)
  • Author(s): Takamitsu Kato, ^*Ryuichi Okayasu, ^*Joel S. Bedford
  • Journal Title: Mutation Research 639 Pages: 108-112
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Role of the major, small, acid-soluble spore proteins and spore-specific and universal DNA repair mechanisms in resistance of Bacillus subtilis spores to ionizing radiation from X rays and high energy charged-particle bombardment (2008)
  • Author(s): Ralf Moeller, Peter Setlow, Gerda Horneck, Thomas Berger, Gunther Reitz, Petra Rettberg, Aidan J. Doherty, Ryuichi Okayasu, Wayne L Nicholson
  • Journal Title: Journal of Bacteriology 190 Pages: 1134-1140
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] High LET heavy ion radiation induces lower numbers of initial chromosome breaks with minimal repair than low LET radiation in normal human cells (2008)
  • Author(s): Eimiko Sekine, Maki Okada, Naruhiro Matsufuji, Dong Yu, Yoshiya Furusawa, ^*Ryuichi Okayasu
  • Journal Title: Mutation Research 652 Pages: 95-101
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Down regulation of BRCA2 causes radio-sensitization of human tumor cells (2008)
  • Author(s): Dong Yu, Eimiko Sekine, Akira, Fujimori, Takahiro Ochiya, ^*Ryuichi Okayasu :
  • Journal Title: Cancer Science 99 Pages: 810-815
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Ionizing radiation downregulates ASPM, a gene responsible for microcephaly in humans (2008)
  • Author(s): Akira Fujimori, Wang Bing, Katsutoshi Suetomi, Eimiko Sekine, Dong Yu, Takamitsu Kato, Sentaro Takahashi, Ryuichi Okayasu, et. al.
  • Journal Title: Biochemical and Biophysical Research Communications 369 Pages: 953-957
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] JS Comparison of the induction and disappearance of DNA double strand breaks and gamma-H2AX foci after irradiation of chromosomes in G1-phase or in condensed metaphase cell, Fundamental and Molecular Mechanisms of Mutagenesis. (2008)
  • Author(s): Kato, T., ^*Okayasu, R., Bedford^*Bedford
  • Journal Title: Mutat. Res. 639 Pages: 108-112
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Role of the major, small, acid-soluble spore proteins and spore-specific and universal DNA repair mechanisms in resistance of Bacillus subtilis spores to ioninzing radiation from X rays and high energy charged-particle bombardment. (2008)
  • Author(s): Moeller, R., Stelow, P., Horneck, G., Berger, T., Reitz, G., Rettberg, P., Doherty, A. J., Okayasu, R., Nicholson, W. L.
  • Journal Title: J. Bacteriol. 190 Pages: 1134-1140
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] High LET heavy ion radiation induces lower numbers of initial chromosome breaks with minimal repair than low LET radiation in normal human cells. (2008)
  • Author(s): Sekine, E., Okada, M., Matsufuji, N., Yu, D., Furusawa, Y. ^*Okayasu, R.
  • Journal Title: Mutat. Res. 652 Pages: 95-101
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Down regulation of BRCA2 causes radio-sensitization of human tumor cells in vitro and in vivo. (2008)
  • Author(s): Yu, D., Sekine, E., Fujimori, A., Ochiya, T., ^*Okayasu, R.
  • Journal Title: Cancer Sci. 99(4) Pages: 810-815
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Ionizing radiation downregulates ASPM, a gene responsible for microcephaly in humans. (2008)
  • Author(s): Fujimori, A, Bing, W., Suetomi, K., Sekine, E., Yu, D., Kato, T., Takahashi, S., Okayasu, R. et. Al
  • Journal Title: Biochem. Biophys. Res. Commun. 369 Pages: 953-957
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Enhancement of chromosomal aberrations in tumor cells with a non-labeled Cu-PTSM and irradiation with Cu K-shell monochromatic X rays. (2007)
  • Author(s): Takakura K, Yaguchi S, Kanansugi Y, Kobayashi K, Okayasu R, Fujibayashi Y.
  • Journal Title: Radiation Protection Dosimetry 122 Pages: 188-194
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Single extreme low dose/low dose rate irradiation causes alteration in lifespan and genome instability in primary human cells. (2007)
  • Author(s): Okada M, Okabe A, Uchihori Y, Kitamura H, Sekine E, Ebisawa S, Suzuki M, Okayasu R.
  • Journal Title: British Journal of Cancer 96 Pages: 1707-1710
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] DNA topoisomerase inhibitor, etoposide, enhances GC-box-dependent promoter activity via Sp1 phosphorylation. (2007)
  • Author(s): Niina I, Uchiumi T, Izumi H, Torigoe T, Wakasugi T, Igarashi T, Miyamoto N, Onitsuka T, Shiota M, Okayasu R, Chijiiwa K, Kohno K.
  • Journal Title: Cancer Science 98 Pages: 858-863
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks : Comparative studies using radiosensitive mutant and wild-type CHO cells (2007)
  • Author(s): Takamitsu Kato, Hatsumi Nagasawa, Christy Warner, ^*Ryuichi Okayasu, Joel S. Bedford
  • Journal Title: International Journal of Radiation Biology 83 Pages: 583-591
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] CELLULAR SIGNAL TRANSDUCTION EVENTS AS A FUNCTION OF LINEAR ENERGY TRANSFER(LET) (2007)
  • Author(s): Furweger Christoph, Michael Hajek, Norbert Vana, R Kodym, Ryuichi Okayasu
  • Journal Title: Radiation Protection Dosimetry 126 Pages: 418-422
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] ENHANCEMENT OF CHROMOSOMAL ABERRATIONS INTUMOR CELLS WITH A NON-LABELED CU-PTSM ANDIRRADIATION WITH CU-K SHELL MONOCHROMATIC X RAYS. (2007)
  • Author(s): Takakura, K., Yaguchi, S., Kanasugi, Y., Kobayashi, K., Okayasu, R., Fujibayashi, Y.
  • Journal Title: Radiat. Protec. Dosimetry 122 Pages: 188-194
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Single extreme low dose/low dose rate irradiation causes alteration in life span and genome instability in primary human cells. (2007)
  • Author(s): Okada, M., Okabe, A., Uchihori, Y., Kitamura, H., Sekine, E., Ebisawa, S., Suzuki, M., ^*Okayasu R.
  • Journal Title: Br. J. Cancer 96 Pages: 1707-1710
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] DNA topoisomerase inhibitor, etoposide, enhances GC-box-dependent promoter activity via Spl phosphorylation. (2007)
  • Author(s): Niina I, Uchiumi T, Izumi H, torigoe T, Wakasugi T, Igarashi T, Miyamoto N, Onitsuka T, Shiota M, Okayasu R, Chijiiwa K, Kohno K.
  • Journal Title: Cancer Sci. 98 Pages: 858-863
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] JS Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks : Comparative studies using radiosensitive mutant and wild-type CHO cells (2007)
  • Author(s): Kato, T., Nagasawa, H., Warner, C., ^*Okayasu R., Bedford^*
  • Journal Title: Int. J. Radiat. Biol. 83 Pages: 583-591
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] CELLULAR SIGNAL TRANSDUCTION EVENTS AS A FUNCTION OF LINEAR ENERGY TRANSFER(LET). (2007)
  • Author(s): Furweger C., Hajek, M., Vana, N., Kodym, R., Okayasu, R.
  • Journal Title: Radiat. Protec. Dosimetry Pages: 1-5
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Repair of DNA damage induced by accelerated heavy ions in mammalian cells proficient and deficient in the Non-homologous End-joining pathway (2006)
  • Author(s): Ryuichi Okayasu, Maki Okada, Atsushi Okabe, Miho Noguchi, Kaoru Takakura, Sentaro Takahashi
  • Journal Title: Radiation Research 165 Pages: 59-67
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The PCC assay can be used to predict radiosensitivity in biopsy cultures irradiated with different types of radiation. (2006)
  • Author(s): Suzuki M, Tsuruoka C, Nakano T, Ohno T, Furusawa Y, Okayasu R.
  • Journal Title: Oncology Report 18 Pages: 1293-1299
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Inhibition of homologous recombination repair in irradiated tumor cells pretreated with Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin. (2006)
  • Author(s): Noguchi M, Yu D, Hirayama R, Ninomiya Y, Sekine E, Kubota N, Ando K, Okayasu R.
  • Journal Title: Biochemistry Biophysics Research Communications 351 Pages: 658-663
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Heavy ion radiation induces complex DNA damage that cannot be efficiently repaired by non-homologous end joining. (2006)
  • Author(s): Okayasu, R., Okada, M, Okabe, A., Noguchi, M., Takakara, K., takahashi, S.
  • Journal Title: Radiat. Res. 165 Pages: 59-67
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The PCC assay can be used to predict radiosensitivity in biopsy cultures irradiated with different types of radiation. (2006)
  • Author(s): Suzuki, M., Tsuruoka, C., Nakano, T., Ohno, Y., Furusawa, Okayasu, R.
  • Journal Title: Oncol. Rep. 16 Pages: 1293-1299
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibition of Homologous Recombination Repair in Irradiated Tumor Cells Pretreated with Hsp90 Inhibitor 17-Allylamino-17-demethoxygeldanamycin. (2006)
  • Author(s): Noguchi, M, Yu, D., Hirayama, R., Ninomiya, Y., Sekine, E., Kubota, N., Ando, K. Okayasu, R.
  • Journal Title: Biochem. Biophys. Res. Commun. 351 Pages: 658-663
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Radio-sensitivity of the cells from amyotrophic lateral sclerosis model mice transfected with human mutant SOD1. (2005)
  • Author(s): Iimuro, R, Takahashi, S, Ito, H, Kubota, Y, Suetomi, K, Sato, H, Okayasu, R.
  • Journal Title: Journal of Radiation Research 46 Pages: 67-73
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Caffeine sensitizes non-dividing human fibroblasts to X-rays by inducing high frequency of misrepair. (2005)
  • Author(s): Kawata, T., Ito, H., Saito, M., Uno, T., Okayasu, R, et. al.
  • Journal Title: Radiation Research 164 Pages: 509-513
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Extremely Low-Dose Ionizing Radiation Upregulates CXC Chemokines in Normal Human Fibroblasts (2005)
  • Author(s): Akira Fujimori, Ryuichi Okayasu, Hiroshi Ishihara, Satoshi Yoshida, Kiyomi Eguchi-Kasai, Kumie Nojima, Satoru Ebisawa, Sentaro Takahashi
  • Journal Title: Cancer Research 65 Pages: 10159-10163
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Radio-sensitivity of the cells from amyotrophic lateral sclerosis model mice transfected with human mutant SOD1 (2005)
  • Author(s): Iimuro, R, Takahashi, S, Ito, H, Kubota, Y, Suetomi, K, Sato, H, Okayasu, R.
  • Journal Title: J. Radiat. Res. 46 Pages: 67-73
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Caffeine sensitizes non-dividing human fibroblasts to X-rays by inducing high frequency of misrepair. (2005)
  • Author(s): Kawata, T., Ito, H., Saito, M., Uno, T., Okayasu, R., Liu, C., Kan'o, M., George, K., Cucinotta, F. A.
  • Journal Title: Radiat. Res. 164 Pages: 509-513
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Extremely low-dose ionizing radiation upregulates CXC chemokines in normal human fibroblasts. (2005)
  • Author(s): Fujjimori, A., Okayasu, R., Ishihara, H., Yoshida, S., Eguchi-Kasai, K., Nojima, K., Ebisawa, S., takahashi, S.
  • Journal Title: Cancer Res. 65 Pages: 10159-10163
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Facilitated detection of chromosome break and repair at low levels of ionizing radiation by addition of wortmannin to G1-type PCC. (2004)
  • Author(s): Okada, M., S. Saito, Okayasu, R.
  • Journal Title: Mutation Research 562 Pages: 11-17
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Facilitated detection of chromosome break and repair at low levels of ionizing radiation by addition of wortmannin to G1-type PCC fusion incubation. (2004)
  • Author(s): Okada M, Saito S, ^*Okayasu R
  • Journal Title: Mutat Res. 562 Pages: 11-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Radiobiology for Heavy-Ion Radiotherapy, (2008)
  • Author(s): Okayasu, R., Kato, T., Noguchi, M., Okada, M., Fujimori, A., Sekine, E.
  • Organizer: NIRS-MD Anderson Symposium on Clinical Issues for Particle Therapy
  • Place of Presentation: Houston,
  • Year and Date: 20080300
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Radiobiology for Heavy-Ion Radiotherapy (2008)
  • Author(s): Okayasu, R., Kato, T., Noguchi, M., Okada, M., Fujimori, A., Sekine, E.
  • Organizer: NIRS-MD Anderson Symposium on Clinical Issues for Particle Therapy
  • Place of Presentation: Houston, USA
  • Year and Date: 20080300
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] BRCA2 siRNAを用いたin vitro, in vivoでの放射線増感 (2008)
  • Author(s): 岡安 隆一、干 冬、関根 絵美子、落谷 孝広、藤森 亮
  • Organizer: 第10回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良
  • Year and Date: 20080200
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 極低線量・低線量率重粒子線1回照射による細胞老化およびゲノム不安定性 (2007)
  • Author(s): 岡安 隆一、岡田 真希、岡部 篤史、関根 絵美子、内堀 幸夫、北村 尚、鈴木 雅雄
  • Organizer: 日本放射線影響学会第50回大会
  • Place of Presentation: 千葉市
  • Year and Date: 20071100
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ATM protein plays a critical role in repairing the complex DNA double strand breaks induces by high LET radiation, (2006)
  • Author(s): Okayasu, R., Okabe, A., Takakura, K.
  • Organizer: Radiation Research Society 53rd Annual Meeting,
  • Place of Presentation: Philadelphia, PA
  • Year and Date: 20061100
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] ATM protein plays a critical role in repairing the complex DNA double strand breaks induced by high LET radiation. (2006)
  • Author(s): Okayasu, R., Okabe, A., Takakura, K.
  • Organizer: Radiation Research Society 53rd Annual Meeting
  • Place of Presentation: Philadelphia, PA USA
  • Year and Date: 20061100
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Comparison of phosphorylation kinetics in DNA repair proteins after exposure to high and low LET radiations, (2006)
  • Author(s): Okayasu, R., Okabe, A., Takakura, K
  • Organizer: The 36th COSPAR Scientific Assembly,
  • Place of Presentation: 北京
  • Year and Date: 20060700
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Comparison of phosphorylation kinetics in DNA repair proteins after exposure to high and low LET radiations. (2006)
  • Author(s): Okayasu, R., Okabe, A., Takakura, K
  • Organizer: The 36th COSPAR Scientific Assembly
  • Place of Presentation: Beijing, China
  • Year and Date: 20060700
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Application of HiCEP, a novel method which can deteck known and unknown gene expression changes after exposure to low dse X-rays, (2006)
  • Author(s): Okayasu, R., Fujimori, A., Takahashi, S.
  • Organizer: The sixth Japan-France workshop on radiobiology and isotopic imaging,
  • Place of Presentation: Paris, France
  • Year and Date: 20060600
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Application of HiCEP, a novel method which can detect known and unknown gene expression changes after exposure to low dose X-rays. (2006)
  • Author(s): Okayasu, R., Fujimori, A., Takahashi, S.
  • Organizer: The sixth Japan-France workshop on radiobiology and isotopic imaging
  • Place of Presentation: Paris, France
  • Year and Date: 20060600
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] High LET radiation uncovers a new aspect of DNA double strand break repair. (2005)
  • Author(s): Okayasu, R., Okada, M., Okabe, A., Noguchi, M., Takahashi, S.
  • Organizer: Japan Radiation Research Society Annual Meeting and The 1st Asian Radiation Research Conference
  • Place of Presentation: Hiroshima, Japan,
  • Year and Date: 20051100
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] High Let radiation uncovers a new aspect of DNA double strand break repair. (2005)
  • Author(s): Okayasu, R., Okada, M., Okabe, A., Noguchi, M., Takahashi, S.
  • Organizer: Japan Radiation Research Society Annual Meeting and The 1st Asian Radiation Research Conference
  • Place of Presentation: Hiroshima, Japan
  • Year and Date: 20051100
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Extreme low dose rate irradiation with heavy ions induces accelerated senescence in primary human cells. (2005)
  • Author(s): Okayasu, R., Okada, M., Okabe, A., Uchihori, Y., Suzuki, M., Takahashi, S.
  • Organizer: The 52nd Radiation Research Society Annual Meeting,
  • Place of Presentation: Denver,
  • Year and Date: 20051000
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Extreme low dose rate irradiation with heavy ions induces accelerated senescence in primary human cells. (2005)
  • Author(s): Okayasu, R., Okada, M., Okabe, A., Uchihori, Y., Suzuki, M., Takahashi, S.
  • Organizer: The 52nd Radiation Research Society Annual Meeting
  • Place of Presentation: Denve, USA
  • Year and Date: 20051000
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] High LET radiation affects non-homologous end joining repair in human cells. (2005)
  • Author(s): Okayasu, R., Okada, M., Okabe, A., Takahashi, S.
  • Organizer: 96th Annual Meeting.
  • Place of Presentation: Anaheim, CA
  • Year and Date: 20050400
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] High LET radiation affects non-homologous end joining repair in human cells. (2005)
  • Author(s): Okayasu, R., Okada, M, Okabe, A., Takahashi, S.
  • Organizer: 96th Annual Meeting, Anaheim
  • Place of Presentation: CA, USA
  • Year and Date: 20050400
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Characteristic studies of non homologous end joining in human cells irradiated with high LET radiation. (2004)
  • Author(s): Okayasu, R., Okada, M., Okabe, A. and Takakura, K
  • Organizer: 35th COSPAR Scientific Assembly
  • Place of Presentation: Paris, France
  • Year and Date: 20040700
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 癌の臨床(6月号の1章執筆) (2007)
  • Author(s): 岡安 隆一、岡田 真希、岡部 篤史、野口 実穂
  • Total Pages: 60(371-375)
  • Publisher: 篠原出版新社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 放射線生物研究 41巻 (1章執筆) (2006)
  • Author(s): 岡安 隆一
  • Total Pages: 126(424-432)
  • Publisher: 放射線生物研究会
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 放射線生物研究 40巻 (1章執筆) (2005)
  • Author(s): 岡安 隆一、末冨 勝敏、高橋 千太郎
  • Total Pages: 114(260-269)
  • Publisher: 放射線生物研究会
  • Description: 「研究成果報告書概要(和文)」より
• [Remarks] 「研究成果報告書概要(和文)」より
  • URL: http://www.nirs.go.jp