Development of MHC-restricted cell therapy using brain-tumor specific antigen and its chemicals

2006 Fiscal Year Final Research Report Summary

• Project/Area Number: 16209044
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Nagoya University
• Principal Investigator: YOSHIDA Jun Nagoya University, Graduate School of Medicine, Professor, 大学院医学系研究科, 教授 (40158449)
• Co-Investigator(Kenkyū-buntansha): WAKABAYASHI Toshihiko Nagoya University, University Hospital, Associate Professor, 医学部附属病院, 助教授 (50220835) ; MIZUNO Masaaki Nagoya University, Graduate School of Medicine, Associate Professor, 大学院医学系研究科, 助教授 (70283439) ; NISHIMURA Takashi Hokkaido University, Institute for Genetic Medicine, Professor, 遺伝子制御研究所, 教授 (30143001) ; TANUMA Sei-ichi Tokyo University of Science, Faculty pf Pharmaceutical Sciences, Professor, 薬学部, 教授 (10142449)
• Project Period (FY): 2004 – 2006
• Keywords: Brain tumor / MHC / Immunotherapy / Cell therapy / ISO / GMP

Research Abstract

We have been developing MHC-restricted cell therapy using brain-tumor specific antigen and its chemicals. At first we identified X-linked X-linked inhibitor of apoptosis protein (XIAP), Aurora-B, c-Jun N-terminal kinase (JNK) and Fas as brain tumor-specific or associated antigens. Thereafter we designed their chemicals using methods of structure-based drug design or genome-information drug design, confirming their biological activities in cultured human glioma cells. Until now we operated genetic medicines in Japan for over 20 years and provided medical services to patients with formidable diseases such as malignant glioma and malignant melanoma. The results were succeeded to The Center for Genetic and Regenerative Medicine (CGRM) in our Hospital, which is an institute to put advanced medicine into practice in Nagoya University Hospital. There we are responsible for process development, validation, and GMP (Good Manufacturing Practice) production of a broad range of novel cell and gene therapies including dendritic cell vaccines, antigen-specific CTLs (cytotoxic T lymphocytes), plasmid DNA, liposomes with plasmid DNA, and bio-materials for regenerative medicine, in support of some clinical trials. In 2006 this CGRM gained ISO (International Organization for Standardization) 9001:2000 and 13485:2003 Accreditation. We became the first academic institute in Japan to achieve the ISO Quality. We opened clinical trials for patients with recurrent malignant glioma using MHC-restricted cells against some brain-tumor specific or associated antigen

Research Products

• [Journal Article] Inhibition of Aurora-B function enhances the antitumor effect of interferon-beta in human glioma cells. (2007)
  • Author(s): Tsuno T, Mizuno M, et al.
  • Journal Title: J Neuro-Oncol. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of CLUAP1 as a human osteosarcoma tumor-associated antigen recognized by the humoral immune system. (2007)
  • Author(s): Ishikura, H, et al.
  • Journal Title: Int J Oncol (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] NKT cells from IL-4-deficient mice are defcecotove in early IFN-γ production in response to α-galactosylceramide. (2007)
  • Author(s): Togashi, Y, et al.
  • Journal Title: Cancer Sci. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Th1 cell adjuvant therapy combined with tumor vaccination : A novel strategy for promoting CTL responses while avoiding the accumulation of Tregs. (2007)
  • Author(s): Zhang, Y, et al.
  • Journal Title: Int. Immunol. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] AsialoGM1^+CD8^+central memory-type T cells in unimmunized mice as novel immunomodulator of IFN-γ-dependent type 1 immunity. (2007)
  • Author(s): Kosaka, A, et al.
  • Journal Title: Int. Immunol. (in press)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunomodulating effect of vitamin D3 derivatives on type-1 cellular immunity. (2007)
  • Author(s): Imazeki, I, et al.
  • Journal Title: Biomedical Research. 27 Pages: 1-9
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Structure basis for the inhibitory mechanism of a novel DNase gamma-specific inhibitor, DR396. (2007)
  • Author(s): Sunaga S, et al.
  • Journal Title: Bioorg. Med. Chem. 14 Pages: 4217-4226
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Inhibition of Aurora-B function enhances the antitumor effect of interferon-beta in human glioma cells. (2007)
  • Author(s): Tsuno T, Mizuno M, Natsume A, Fujita M, Nakahara N, Wakabayashi T, Satoh Y, Inagaki M, Yoshida J
  • Journal Title: J Neuro-Oncol. (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of CLUAP1 as a human osteosarcoma tumor-associated antigen recognized by the humoral immune system. (2007)
  • Author(s): Ishikura, H., Ikeda, H., Abe, H., Ohkuri, T., Hiraga, H., Isu, K., Tsukahara, T., Sato, N., Kitamura, H., Iwasaki, N., Takeda, N., Minami, A., Nishimura, T.
  • Journal Title: Int J Oncol (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] NKT cells from IL-4-deficient mice are defcecotove in early IFN-y production in response to a ・galactosylceramide. (2007)
  • Author(s): Togashi, Y., Chamoto, K., Wakita, D., Tsutsumi, N., Iwakura, Y., Matsubara, N., Kitamura, H., Nishimura, T.
  • Journal Title: Cancer Sci (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Th1 cell adjuvant therapy combined with tumor vaccination : A novel strategy for promoting CTL responses while avoiding the accumulation of Tregs. (2007)
  • Author(s): Zhang, Y., Wakita, D., Chamoto, K., Narita, Y., Matsubara, N., Kitamura, H., Nishimura, T.
  • Journal Title: Int. Immunol. (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] AsialoGMl+CD8+centr al memory-type T cells in unimmunized mice as novel immunomodulator of IFN-y-dependent type 1 immunity. (2007)
  • Author(s): Kosaka, A., Wakita, D., Matsubara, N., Togashi, Y., Nishimura, S., Kitamura, H., Nishimura, T.
  • Journal Title: Int. Immunol (In press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] EGFR mutation in patients with brain metastases from lung cancer : correlation with the efficacy of gefitinib. (2006)
  • Author(s): Shimato S, et al.
  • Journal Title: Neuro-Oncol. 8(2) Pages: 137-144
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Brain metastases from apocrine carcinoma of the scalp : case report (2006)
  • Author(s): Shimato S, et al.
  • Journal Title: J Neuro-Oncol. 77(3) Pages: 285-289
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human gene therapy for malignant gliomas. (2006)
  • Author(s): Wakabayashi T
  • Journal Title: Minimally Invasive Neurosurgery and Multidisciplinary Neurotraumatology. Pages: 1191-1123
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Antitumor activity of chimeric immunoreceptor gene-modified Tcl and Thl cells against autologous carcinoembryonic antigen-expressing colon cancer cells. (2006)
  • Author(s): Sasaki, T, et al.
  • Journal Title: Cancer Sci 97 Pages: 920-927
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Interleukin-12-responding asialoGM1CD8 central memory-type T cells as precursor cells for interferon-gamma- producing killer T cells (2006)
  • Author(s): Kosaka, A, et al.
  • Journal Title: Cancer Sci. 97 Pages: 1236-1241
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of the MAGE-A4 and NY-ESO-1 cancer-testis antigens and T cell infiltration in non-small cell lung carcinoma and their prognostic significance. (2006)
  • Author(s): Yoshida, N, et al.
  • Journal Title: Int J Oncol. 28 Pages: 1089-1098
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An essential role of antigen-presenting cell/T-helper type 1 cell-cell interactions in draining lymph node during complete eradication of class II-negative tumor tissue by T-helper type 1 cell therapy. (2006)
  • Author(s): Chamoto, K, et al.
  • Journal Title: Cancer Res. 66 Pages: 1809-1817
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] An indispensable role of type-1 IFNs for inducing CTL-mediated complete eradication of established tumor tissue by CpG-liposome co-encapsulated with model tumor antigen. (2006)
  • Author(s): Wakita, D, et al.
  • Journal Title: Int Immunol. 18 Pages: 425-434
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] lalpha, 25-Dihydroxyvitamin D3 downmodulates the functional differentiation of Thl cytokine-conditioned bone marrow-derived dendritic cells beneficial for cytotoxic Tlymphocyte generation. (2006)
  • Author(s): Matsuzaki, J, et al.
  • Journal Title: Cancer Sci. 97 Pages: 139-147
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A new fluorescent probe for zinc (II) : an 8-hydroxy- 5-N, N-dimethyl aminosulfonylquinoline-pendant 1, 4, 7, 10-tetra azacyclododecane. (2006)
  • Author(s): Aoki S, et al.
  • Journal Title: Chemistry 12 Pages: 9066-9080
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Action of apoptotic endonuclease DNase gamma on naked DNA and chromatin substrates. (2006)
  • Author(s): Mizuta R, et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 345 Pages: 560-567
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] cDNA cloning and functional characterization of Xenopus laevis DNase gamma. (2006)
  • Author(s): Shiokawa D, et al.
  • Journal Title: Apoptosis 11 Pages: 555-562
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] CD28-dependent differentiation into the effector/memory phenotype is essential for induction of arthritis in interleukin-1 receptor antagonist-deficient mice. (2006)
  • Author(s): Kotani M, et al.
  • Journal Title: Arthritis Rheum. 54 Pages: 473-481
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The novel beta-secretase inhibitor KMI-429 reduces amyloid beta peptide production in amyloid precursor protein transgenic and wild-type mice. (2006)
  • Author(s): Asai M, et al.
  • Journal Title: J. Neurochem. 96 Pages: 533-540
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] EGFR mutation in patients with brain metastases from lung cancer : correlation with the efficacy of gefitinib. (2006)
  • Author(s): Shimato S, Mitsudomi T, Kosaka T, Yatabe Y, Wakabayashi T, Mizuno M, Nakahara N, Nakahara N, Hatano H, Natsume A, Ishii D, Yoshida J
  • Journal Title: Neuro-Oncol. 8(2) Pages: 137-44
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Brain metastases from apocrine carcinoma of the scalp : case report. (2006)
  • Author(s): Shimato S, Wakabayashi T, Mizuno M, Nakahara N, Hatano H, Natsume A, Ishii D, Hasegawa Y, Hyodo I, Nagasaka T, Yoshida J
  • Journal Title: J Neuro-Oncol. 77(3) Pages: 285-289
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human gene therapy for malignant gliomas. Kanno T (Ed) Minimally Invasive Neurosurgery and Multidisciplinary Neurotraumatology. (2006)
  • Author(s): Wakabayashi T, Yoshida J, Mizuno M, Fujii M, Kajita Y, Nakahara N, Hatano H
  • Journal Title: Springer-Verlag (Tokyo) Pages: 1191-123
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Antitumor activity of chimeric immunoreceptor gene-modified Tc1 and Th1 cells against autologous carcinoembryonic antigen-expressing colon cancer cells. (2006)
  • Author(s): Sasaki, T., Ikeda, H., Sato, M., Ohkuri, T., Abe, H., Kuroki, M., Onodera, M., Miyamoto, M., Kondo, S., Nishimura, T.
  • Journal Title: Cancer Sci 97 Pages: 920-927
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interleukin-12-responding asialoGM1CD8 central memory-type T cells as precursor cells for interferon-gamma-producing killer T cells. (2006)
  • Author(s): Kosaka, A., Lee, U., Wakita, D., Matsubara, N., Togashi, Y., Nishimura, S.I., Kitamura, H., Nishimura, T.
  • Journal Title: Cancer Sci 97 Pages: 1231-1241
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of the MAGE-A4 and NY-ESO-1 cancer-testis antigens and T cell infiltration in non-small cell lung carcinoma and their prognostic significance. (2006)
  • Author(s): Yoshida, N., Abe, H., Ohkuri, T., Wakita, D., Sato, M., Noguchi, D., Miyamoto, M., Morikawa, T., Kondo, S., Ikeda, H., Nishimura, T.
  • Journal Title: Int J Oncol 28 Pages: 1089-1098
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunomodulating effect of vitamin D3 derivatives on type-1 cellular immunity (2006)
  • Author(s): Imazeki, I., Matsuzaki, J., Tsuji K., Nishimura, T.
  • Journal Title: Biomedical Research 27 Pages: 1-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An essential role of antigen-presenting cell/T-helper type 1 cell-cell interactions in draining lymph node during complete eradication of class II-negative tumor tissue by T-helper type 1 cell therapy. (2006)
  • Author(s): Chamoto, K., Wakita, D., Narita, Y., Zhang, Y., Noguchi, D., Ohnishi, H., Iguchi, T., Sakai, T., Ikeda, H., Nishimura, T.
  • Journal Title: Cancer Res 66 Pages: 1809-1817
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] An indispensable role of type-1 IFNs for inducing CTL-mediated complete eradication of established tumor tissue by CpG-liposome co-encapsulated with model tumor antigen. (2006)
  • Author(s): Wakita, D., Chamoto, K, Zhang, Y., Narita, Y., Noguchi, D., Ohnishi, H., Iguchi, T., Sakai, T., Ikeda, H., Nishimura, T.
  • Journal Title: Int Immunol 18 Pages: 425-434
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] lalpha,25-Dihydroxyvitamin D3 downmodulates the functional differentiation of Th1 cytokine-conditioned bone marrow-derived dendritic cells beneficial for cytotoxic T lymphocyte generation. (2006)
  • Author(s): Matsuzaki, J., Tsuji, T., Zhang, Y., Wakita, D., Imazeki, I., Sakai, T., Ikeda, H., Nishimura, T.
  • Journal Title: Cancer Sci 97 Pages: 139-147
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A new fluorescent probe for zinc (II) : an 8-hydroxy-5-N, N-dimethylaminosulfonylquinoline-pendant 1, 4, 7, 10-tetra azacyclododecane. (2006)
  • Author(s): Aoki S., Sakurama K., Matsuo N., Yamada Y., Takasawa R., Tanuma S., Shiro M., Takeda K., Kimura E.
  • Journal Title: Chemistry 12 Pages: 9066-9080
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Action of apoptotic endonuclease DNase gamma on naked DNA and chromatin substrates. Biochem. Biophys. (2006)
  • Author(s): Mizuta R., Mizuta M., Araki S., Shiokawa D., Tanuma S., Kitamura D.
  • Journal Title: Res. Commun. 345 Pages: 560-567
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure basis for the inhibitory mechanism of a novel DNase gamma-specific inhibitor, DR396. Bioorg. (2006)
  • Author(s): Sunaga S., Yoshimori A., Shiokawa D., Tanuma S.
  • Journal Title: Med. Chem. 14 Pages: 4217-4226
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] cDNA cloning and functional characterization of Xenopus laevis DNase gamma. (2006)
  • Author(s): Shiokawa D., Hatanaka T., Hatanaka M., Shika Y., Nishikawa A., Tanuma S.
  • Journal Title: Apoptosis 11 Pages: 555-562
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] CD28-dependent differentiation into the effector/memory phenotype is essential for induction of arthritis in interleukin-1 receptor antagonist-deficient mice. (2006)
  • Author(s): Kotani M., Hirata K., Ogawa S., Habiro K., Ishida Y., Tanuma S., Horai R., Iwakura Y., Kishimoto H., Abe R.
  • Journal Title: Arthritis Rheum. 54 Pages: 473-481
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The novel beta-secretase inhibitor KMI-429 reduces amyloid beta peptide production in amyloid precursor protein transgenic and wild-type mice. (2006)
  • Author(s): Asai M., Hattori C., Iwata N., Saido T.C., Sasagawa N., Szabo B., Hashimoto Y., Maruyama K., Tanuma S., Kiso Y., Ishiura S.
  • Journal Title: J. Neurochem. 96 Pages: 533-540
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] IFN-beta down-regulates the expression of DNA repair gene MGMT and sensitizes resistant glioma cells to temozolomide. (2005)
  • Author(s): Natsume A, et al.
  • Journal Title: Cancer Res 65 Pages: 7573-7579
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of a functional cDNA array for evaluation of the Th1/Th2 balance. Immunol Lett, 101; 95-103, 2005. (2005)
  • Author(s): Yamaguchi A, et al.
  • Journal Title: Immunol Lett 101 Pages: 95-103
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation of tumor-specific, HLA class I-restricted human Th1 and Tc1 cells by cell engineering with tumor peptide-specific T-cell receptor genes. (2005)
  • Author(s): Tsuji, T, et al.
  • Journal Title: Blood 106 Pages: 470-476
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro. (2005)
  • Author(s): Kijima, M, et al.
  • Journal Title: Int J Oncol 26 Pages: 679-684
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differential apoptotic pathways in human keratinocyte HaCaT cells exposed to UVB and UVC. (2005)
  • Author(s): Takasawa R, et al.
  • Journal Title: Apoptosis 10 Pages: 1121-1130
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Physical and biochemical properties of mammalian DNase X proteins : non-AUG translation initiation of porcine and bovine mRNAs for DNase X. (2005)
  • Author(s): Shiokawa D, et al.
  • Journal Title: Biochem. J 392 Pages: 511-517
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a novel gene family, paralogs of inhibitor of apoptosis proteins present in plants, fungi, and animals. (2005)
  • Author(s): Higashi K., et al.
  • Journal Title: Apoptosis 10 Pages: 471-480
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Identification of a drug target motif : an anti-tumor drug NK109 interacts with a PNxxxxP. (2005)
  • Author(s): Morohashi K, et al.
  • Journal Title: Biochem. Pharmacol 70 Pages: 37-46
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Structure-based design of an agonistic peptide targeting Fas. (2005)
  • Author(s): Yoshimori A, et al.
  • Journal Title: Apoptosis 10 Pages: 323-329
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Analysis of the susceptibility of CD57 T cells to CD3-mediated apoptosis. (2005)
  • Author(s): Shinomiya N, et al.
  • Journal Title: Clin. Exp. Immunol. 139 Pages: 268-278
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Involvement of DNase gamma in the resected double-strand DNA breaks in immunoglobulin genes. (2005)
  • Author(s): Okamoto M., et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 327 Pages: 76-83
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Immunosteroid as a regulator for Th1/Th2 balance : its possible role in autoimmune diseases. (2005)
  • Author(s): Matsuzaki, J, et al.
  • Journal Title: Autoimmunity 38 Pages: 369-375
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] IFN-b down-regulates the expression of DNA repair gene MGMT and sensitizes resistant glioma cells to temozolomide. (2005)
  • Author(s): Natsume A, Ishii D, Wakabayashi T, Tsuno T, Hatano H, Mizuno M, Yoshida J
  • Journal Title: Cancer Res. 65817 Pages: 7573-7579
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of a functional cDNA array for evaluation of the Thl/Th2 balance. (2005)
  • Author(s): Yamaguchi, A., Koda, T., Abe, H., Sato, M., Li, J., Sakai, T., Togashi, Y., Shinohara, Y., Ikeda, H., Nishimura, T.
  • Journal Title: Immunol Lett 101 Pages: 95-103
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation of tumor-specific, HLA class I-restricted human Th1 and Tc1 cells by cell engineering with tumor peptide-specific T-cell receptor genes. (2005)
  • Author(s): Tsuji, T., Yasukawa, M., Matsuzaki, J., Ohkuri, T., Chamoto, K., Wakita, D., Azuma, T., Niiya, H., Miyoshi, H., Kuzushima, K., Oka, Y., Sugiyama, H., Ikeda, H., Nishimura, T.
  • Journal Title: Blood 106 Pages: 470-476
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Natural killer cells play a role in MHC class I in vivo induction in tumor cells that are MHC negative in vitro. (2005)
  • Author(s): Kijima, M., Saio, M., Oyang, G.F., Suwa, T., Miyauchi, R., Kojima, Y., Imai, H., Nakagawa, J., Nonaka, K., Umemura, N., Nishimura, T., Takami, T.
  • Journal Title: Int J Oncol 26 Pages: 679-684
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential apoptotic pathways in human keratinocyte HaCaT cells exposed to UVB and UVC. (2005)
  • Author(s): Takasawa R., Nakamura H., Mori T., Tanuma S.
  • Journal Title: Apoptosis 10 Pages: 1121-1130
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Physical and biochemical properties of mammalian DNase X proteins : non-AUG translation initiation of porcine and bovine mRNAs for DNase X. (2005)
  • Author(s): Shiokawa D., Shika Y., Saito K., Yamazaki K., Tanuma S.
  • Journal Title: Biochem. J. 392 Pages: 511-517
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a novel gene family, paralogs of inhibitor of apoptosis proteins present in plants, fungi, and animals. (2005)
  • Author(s): Higashi K., Takasawa R., Yoshimori A., Goh T., Tanuma S., Kuchitsu K.
  • Journal Title: Apoptosis 10 Pages: 471-480
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Identification of a drug target motif an anti-tumor drug NK109 interacts with a PNxxxxP. Biochem. (2005)
  • Author(s): Morohashi K., Yoshino A., Yoshimori A., Saito S., Tanuma S., Sakaguchi K., Sugawara F.
  • Journal Title: Pharmacol. 70 Pages: 37-46
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Structure-based design of an agonistic peptide targeting Fas. (2005)
  • Author(s): Yoshimori A., Takasawa R.Hayakawa A., Mizuno M., Yoshida J., Tanuma S.
  • Journal Title: Apoptosis 10 Pages: 323-329
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Seki S. Analysis of the susceptibility of CD57 T cells to CD3-mediated apoptosis. (2005)
  • Author(s): Shinomiya N., Koike Y., Koyama H., Takayama E., Habu Y., Fukasawa M., Tanuma S.
  • Journal Title: Clin. Exp. Immunol. 139 Pages: 268-278
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Involvement of DNase gamma in the resected double-strand DNA breaks in immunoglobulin genes. Biochem. (2005)
  • Author(s): Okamoto M., Okamoto N., Yashiro H., Shiokawa D., Sunaga S., Yoshimori A., Tanuma S., Kitamura D.
  • Journal Title: Biophys. Res. Commun. 327 Pages: 76-83
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Immunosteroid as a regulator for Thl/Th2 balance : its possible role in autoimmune diseases. (2005)
  • Author(s): Matsuzaki, J., Tsuji, T., Imazeki, I., Ikeda, H., Nishimura, T.
  • Journal Title: Autoimmunity 38 Pages: 369-375
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Interferon-beta gene therapy for cancer : basic research to clinical application. (2004)
  • Author(s): Yoshida J, et al.
  • Journal Title: Cancer Sci 95(11) Pages: 858-865
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Aurora-B dysfunction of multinucleated giant cells in glioma detected by site-specific phosphorylated antibodies. (2004)
  • Author(s): Fujita M, et al.
  • Journal Title: J Neurosurg 101 Pages: 1012-1017
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Vaccination with Tumor cell Lysate-pulsed Dendritic Cells Augments the Effect of IFN-β Gene Therapy for Malignant Glioma in Experimental Mouse Intracranial Glioma. (2004)
  • Author(s): Saito R, et al.
  • Journal Title: Int J Cancer 111 Pages: 777-782
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Application of genetically engineered tumor-specific Th1 cells to adoptive tumor immunotherapy. (2004)
  • Author(s): Tsuji, T, et al.
  • Journal Title: Annals of Cancer Research and Therapy 12 Pages: 71-86
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Liposome-encapsulated CpG oligodeoxynucleotides as a potent adjuvant for inducing type 1 innate immunity. (2004)
  • Author(s): Suzuki, Y, et al.
  • Journal Title: Cancer Res 64 Pages: 8754-8760
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The critical role of type-1 innate and acquired immunity in tumor immunotherapy. (2004)
  • Author(s): Ikeda, H, et al.
  • Journal Title: Cancer Sci 95 Pages: 697-703
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Unexpected role of TNF-α in graft versus host reaction (GVHR) : donor-derived TNF-α suppresses GVHR via inhibition of IFN-γ -dependent donor type-1 immunity. (2004)
  • Author(s): Yamamoto, S, et al.
  • Journal Title: Int Immunol 16 Pages: 811-817
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] IFN-γ-induced SOCS-1 regulates STAT6-dependent eotaxin production triggered by IL-4 and TNF-α. (2004)
  • Author(s): Sato, T, et al.
  • Journal Title: Biochem Biophys Res Commun 314 Pages: 468-475
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] STAT6-mediated signaling in Th2-dependent allergic asthma : critical role for the development of eosinophilia, airway hyper-responsiveness and mucus hypersecretion, distinct from its role in Th2 differentiation. (2004)
  • Author(s): Hoshino, A. et al.
  • Journal Title: Int Immunol 16 Pages: 1497-1505
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation and targeting of human tumor-specific Tc1 and Th1 cells transduced with a lentivirus containing a chimeric immunoglobulin T-cell receptor. (2004)
  • Author(s): Gyobu, H, et al.
  • Journal Title: Cancer Res 64 Pages: 1490-1495
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation of leukemia-specific T-helper type 1 cells applicable to human leukemia cell-therapy. (2004)
  • Author(s): Fujimura, T, et al.
  • Journal Title: Immunol Lett 93 Pages: 17-25
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Potentiation of tumor eradication by adoptive immunotherapy with T-cell receptor gene-transduced T-helper type 1 cells. (2004)
  • Author(s): Chamoto, K, et al.
  • Journal Title: Cancer Res 64 Pages: 386-390
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A novel inhibitor that protects apoptotic DNA fragmentation catalyzed by DNase gamma. (2004)
  • Author(s): Sunaga S, et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 325 Pages: 1292-1297
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of Smubp-2 as a mouse mammary tumor virus promoter-binding protein. (2004)
  • Author(s): Uchiumi F. et al.
  • Journal Title: Biochem. Biophys. Res. Commun. 321 Pages: 355-363
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genomic drug discovery for apoptosis regulation using a new computer screening amino acid complement wave method. (2004)
  • Author(s): Tanuma S. et al.
  • Journal Title: Biol. Pharm. Bull. 27 Pages: 968-973
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Characterization of the human DNAS1L2 gene and the molecular mechanism for its transcriptional activation induced by inflammatory cytokines. (2004)
  • Author(s): Shiokawa D, et al.
  • Journal Title: Genomics 84 Pages: 95-105
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Differential DNases are selectively used in neuronal apoptosis depending on the differentiation state. (2004)
  • Author(s): Shiokawa D, et al.
  • Journal Title: Cell Death Differ. 11 Pages: 1112-1120
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A novel method for evaluation and screening of caspase inhibitory peptides by the amino acid positional fitness score. (2004)
  • Author(s): Yoshimori A, et al.
  • Journal Title: BMC Pharmacol. 4 Pages: 7
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. (2004)
  • Author(s): Higami Y., et al.
  • Journal Title: Cell Tissue Res. 316 Pages: 403-407
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Aging increases DNase gamma, an apoptosis-related endonuclease, in rat liver nuclei : effect of dietary restriction. (2004)
  • Author(s): Tanaka K. et al.
  • Journal Title: Exp. Gerontol. 39 Pages: 195-202
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Monitoring apoptosis with fluorescent Zn2+-indicators. (2004)
  • Author(s): Kimura E, et al.
  • Journal Title: Sci. STKE Pages: PL7
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human gene therapy for malignant gliomas (glioblastomamulti forme and anaplastic astrocytoma) by in vivo transduction with human β-interferon gene using cationic liposomes. (2004)
  • Author(s): Yoshida J, et al.
  • Journal Title: Hum Gene Ther 15 Pages: 77-86
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Interferon-beta gene therapy for cancer : basic research to clinical application. (2004)
  • Author(s): Yoshida J, Mizuno M, Wakabayashi T
  • Journal Title: Cancer Sci 95(11) Pages: 858-865
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aurora-B dysfunction of multinucleated giant cells in glioma detected by site-specific phosphorylated antibodies. (2004)
  • Author(s): Fujita M, Mizuno M, Wakabayashi T, Yoshida J
  • Journal Title: JNS 101 Pages: 1012-1017
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Vaccination with Tumor cell Lysate-pulsed Dendritic Cells Augments the Effect of IFN-β Gene Therapy for Malignant Glioma in Experimental Mouse Intracranial Glioma. (2004)
  • Author(s): Saito R, Mizuno M, Nakahara N, Tsuno T, Kumabe T, Yoshimoto T, Yoshida J
  • Journal Title: Int J Cancer 111 Pages: 777-782
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Application of genetically engineered tumor-specific Th1 cells to adoptive tumor immunotherapy. (2004)
  • Author(s): Tsuji, T., Chamoto, K., Gyobu, H., Ikeda.H., Nishimura, T.
  • Journal Title: Annals of Cancer Research and Therapy 12 Pages: 71-86
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Liposome-encapsulated CpG oligodeoxynucleotides as a potent adjuvant for inducing type 1 innate immunity. (2004)
  • Author(s): Suzuki, Y., Wakita, D., Chamoto, K., Narita, Y, Tsuji, T., Takeshima, T., Gyobu, H., Kawarada, Y., Kondo, S., Akira, S., Katoh, H., Ikeda, H., Nishimura, T.
  • Journal Title: Cancer Res 64 Pages: 8754-8760
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The critical role of type-1 innate and acquired immunity in tumor immunotherapy. (2004)
  • Author(s): Ikeda, H., Chamoto, K., Tsuji, T., Suzuki, Y., Wakita, D., Takeshima, T., Nishimura, T.
  • Journal Title: Cancer Sci 95 Pages: 697-703
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Unexpected role of TNF-a in graft versus host reaction (GVHR) : donor-derived TNF-a suppresses GVHR via inhibition of IFN-y-dependent donor type-1 immunity. (2004)
  • Author(s): Yamamoto, S., Tsuji, T., Matsuzaki, J., Zhange, Y., Chamoto, K., Kosaka, A., Togashi, Y., Sekikawa, K., Sawada, K.I., Takeshima, T., Koike, T., Nishimura, T.
  • Journal Title: Int Immunol 16 Pages: 811-817
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] IFN-y-induced SOCS-1 regulates STAT6-dependent eotaxin production triggered by IL-4 and TNF-a. (2004)
  • Author(s): Sato, T., Saito, R., Jinushi, T., Tsuji, T., Matsuzaki, J., Koda, T., Nishimura, S., Takeshima, H., Nishimura, T.
  • Journal Title: Biochem Biophys Res Commun 314 Pages: 468-475
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] STAT6-mediated signaling in Th2-dependent allergic asthma : critical role for the development of eosinophilia, airway hyper-responsiveness and mucus hypersecretion, distinct from its role in Th2 differentiation. (2004)
  • Author(s): Hoshino, A., Tsuji, T., Matsuzaki, J., Jinushi, T., Ashino, S., Teramura, T., Chamoto, K., Tanaka, Y., Asakura, Y., Sakurai, T., Mita, Y., Takaoka, A., Nakaike, S., Takeshima, T., Ikeda, H., Nishimura, T.
  • Journal Title: Int Immunol 16 Pages: 1497-1505
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation and targeting of human tumor-specific Tc1 and Th1 cells transduced with a lentivirus containing a chimeric immunoglobulin T-cell receptor. (2004)
  • Author(s): Gyobu, H., Tsuji, T., Suzuki, Y., Ohkuri, T., Chamoto, K., Kuroki, M., Miyoshi, H., Kawarada, Y., Katoh, H., Takeshima, T., Nishimura, T.
  • Journal Title: Cancer Res 64 Pages: 1490-1495
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation of leukemia-specific T-helper type 1 cells applicable to human leukemia cell-therapy. (2004)
  • Author(s): Fujimura, T., Chamoto, K., Tsuji, T., Sato, T., Yokouchi, H., Aiba, S., Tagami, H., Tanaka, J., Imamura, M., Togashi, Y., Koda, T., Nishimura, T.
  • Journal Title: Immunol Lett 93 Pages: 17-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Potentiation of tumor eradication by adoptive immunotherapy with T-cell receptor gene-transduced T-helper type 1 cells. (2004)
  • Author(s): Chamoto, K., Tsuji, T., Funamoto, H., Kosaka, A., Matsuzaki, J., Sato, T., Abe, H., Fujio, K., Yamamoto, K., Kitamura, T., Takeshima, T., Togashi, Y., Nishimura, T.
  • Journal Title: Cancer Res 64 Pages: 386-390
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel inhibitor that protects apoptotic DNA fragmentation catalyzed by DNase gamma. Biochem. Biophys. (2004)
  • Author(s): Sunaga S., Kobayashi T., Yoshimori A., Shiokawa D., Tanuma S.
  • Journal Title: Res. Commun. 325 Pages: 1292-1297
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of Smubp-2 as a mouse mammary tumor virus promoter-binding protein. Biochem. Biophys. (2004)
  • Author(s): Uchiumi F., Komuro M., Mizuta R., Tanuma S.
  • Journal Title: Res. Commun. 321 Pages: 355-363
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genomic drug discovery for apoptosis regulation using a new computer screening amino acid complement wave method. Biol. (2004)
  • Author(s): Tanuma S., Yoshimori A., Takasawa R.
  • Journal Title: Pharm. Bull. 27 Pages: 968-973
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Characterization of the human DNAS1L2 gene and the molecular mechanism for its transcriptional activation induced by inflammatory cytokines. (2004)
  • Author(s): Shiokawa D., Matsushita T., Kobayashi T., Matsumoto Y., Tanuma S.
  • Journal Title: Genomics 84 Pages: 95-105
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Differential DNases are selectively used in neuronal apoptosis depending on the differentiation state. (2004)
  • Author(s): Shiokawa D., Tanuma S.
  • Journal Title: Cell Death Differ. 11 Pages: 1112-1120
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel method for evaluation and screening of caspase inhibitory peptides by the amino acid positional fitness score. (2004)
  • Author(s): Yoshimori A., Takasawa R., Tanuma S.
  • Journal Title: BMC Pharmacol. 4 Pages: 7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Shimokawa I. Expression of DNase gamma during Fas-independent apoptotic DNA fragmentation in rodent hepatocytes. (2004)
  • Author(s): Higami Y, Tsuchiya T., To K., Chiba T., Yamaza H., Shiokawa D., Tanuma S.
  • Journal Title: Cell Tissue Res. 316 Pages: 403-407
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Shimokawa I. Aging increases DNase gamma, an apoptosis-related endonuclease, in rat liver nuclei : effect of dietary restriction. Exp. (2004)
  • Author(s): Tanaka K., Higami Y, Tsuchiya T., Shiokawa D., Tanuma S., Ayabe H.
  • Journal Title: Gerontol. 39 Pages: 195-202
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Monitoring apoptosis with fluorescent Zn2+-indicators. (2004)
  • Author(s): Kimura E., Takasawa R., Tanuma S., Aoki S.
  • Journal Title: Sci. STKE. 2004 Pages: PL7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human gene therapy for malignant gliomas (Glioblastoma multiforme and anaplastic astrocytoma) by in vivo transduction with human interferon beta gene using cationic liposomes. (2004)
  • Author(s): Yoshida J, Mizuno M, Fujii M, Kajita Y, Nakahara N, Hatano M, Saito R, Nobayashi M, Wakabayashi T
  • Journal Title: Human gene therapy 15 Pages: 77-86
  • Description: 「研究成果報告書概要(欧文)」より