2005 Fiscal Year Final Research Report Summary
Ultrasound Thrombolysis Using Liposomal Nanobubbles Targeted at Activated Platelets
| Project/Area Number |
16300176
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Medical systems
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| Research Institution | Saitama Medical University |
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Principal Investigator |
NISHIOKA Toshihiko Saitama Medical University, School of Medicine, Assistant professor, 医学部, 講師 (10364746)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Osamu Saitama Medical University, School of Medicine, Instructor, 医学部, 助手 (90364794)
MASAKI Nobuyuki Saitama Medical University, School of Medicine, Instructor, 医学部, 助手 (00364795)
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| Project Period (FY) |
2004 – 2005
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| Keywords | ultrasound / targeted nanobubble / liposome / RGD peptide |
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| Research Abstract |
OBJECTIVES
The aim of this study was to examine the enhancing effect of thrombus-targeted nanobubbles (NBs) on both ultrasonic imaging and disruption of the thrombus in vitro and in vivo.
BACKGROUND
Low frequency ultrasound (USD) in combination with non-specific microbubbles has been reported to recanalize thrombotically occluded arteries in animal models.
METHODS
In vitro : Ultrasound images of thrombus were recorded and digitized before and after the administration of thrombus-targeted NBs or non-targeted NBs. The imaged clot was exposed to low frequency USD for 5 minutes. In vivo : Rabbit ilio-femoral arteries were thrombotically occluded, and ultrasound images of thrombus were recorded and digitized before and after intravenous injection of targeted NBs (n=10) or non-targeted NBs (n=10). External low frequency USD was applied to the thrombotically occluded arteries for 60 minutes. In another 10 rabbits, recombinant tissue plasminogen activator (rt-PA) was intravenously administered.
RESULTS
In vitro : The mean pixel gray-scale level and weight reduction rate of the clot with targeted NBs were significantly higher than controls and non-targeted NBs. In vivo : The echo intensity of the arterial thrombus significantly increased after targeted NBs administration. TIMI grade 3 flow was present in a significantly higher number of rabbits with USD and targeted NBs than in rabbits with USD, non-targeted NBs, and rt-PA therapy. The time to reperfusion was shorter when USD was given with targeted NBs compared to being given with rt-PA therapy.
CONCLUSIONS :
Thrombus-targeted NBs appear to enhance both ultrasonic visualization and disruption of thrombus both in vitro and in vivo.
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Research Products
(4 results)
