Molecular and cellular mechanisms for abscisic acid biosynthesis and transport in response to water stress in plants

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16370026
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物生理・分子
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: KOSHIBA Tomokazu Tokyo Metropolitan University, Biological Sciences, Professor, 都市教養学部・理工学系, 教授 (80117704)
• Co-Investigator(Kenkyū-buntansha): OKAMOTO Takeshi Tokyo Metropolitan University, Biological Sciences, Associate Professor, 都市教養学部・理工学系, 准教授 (50285095) ; SEO Mitsunori Riken, Yokohama Institute, Ph.D.Resarcher, 生殖制御研究グループ, 基礎科学特別研究員
• Project Period (FY): 2004 – 2005
• Keywords: Abscisic acid / Biosynthesis / Transport / Water stress / Arabidosis thaliana / Vascular system

Research Abstract

The accumulation of abscisic acid (ABA) is essential for the response to drought stress in plants. Arabidopsis 9-cis-epoxycarotenoid dioxygenase 3 (AtNCED3) is known to act as a rate-limiting enzyme during stress-induced ABA biosynthesis. The expression of the AtNCED3 gene is up-regulated under water stress, which leads to the rapid accumulation of endogenous ABA in Axabidopsis. However, we have no information about the localization of AtNCED3 and therefore the precise site(s) of ABA biosynthesis remains to be determined. In the present study, we performed expression analysis of three important ABA biosynthetic enzymes, AtNCED3, AtABA2 and AAO3, using specific antibodies against each these enzymes. The results revealed thait the expression of AtABA2 and AAO3 was mainly in the vascular tissues of rosette veins in turgid plants and their patterns showed no significant changes after dehydration treatment. On the other hand, AtNCED3 could not be observed in any region of turgid plants, wbile drought-induced expression of AtNCED3 appeared in vascular region of the leaf veins. The localization patterns of AtNCED3, AtABA2 and AAO3 observed in serial sections of water-stressed rosette leaves indicated that these three enzymes were co-localized to the same cells of vascular parenchyma surrounding xylem and phloem. In addition, AtNCED3 was expressed in chloroplast of parenchyma cells adjacent to xylem vessels. These results suggest that drought induced ABA production occurs rapidly in vascular parenchyma cells, which may directly perceive the change in water potential of xylem and/or phloem upon exposure of water deficient stress. This is the first finding that shows close interaction between water stress perception and ABA biosynthesis in the vascular tissues in plants.

Research Products

• [Journal Article] Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates tha ABA synthesised in the endosperm is involved in the induction of seed dormancy (2006)
  • Author(s): Lefebver, V., Morth, H., Frey, A., Sotta, B., Seo, M., Okamoto, M., Nambara, E., Marion-Poll
  • Journal Title: Plant Journal 45 Pages: 309-319
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] オーキシン、アブシジン酸性合成の分子生物的研究(学会賞(受賞業績)) (2006)
  • Author(s): 小柴共一
  • Journal Title: 植物の生長調節 (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] CYP7O7A3, a major ABA 8-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana. (2006)
  • Author(s): Umezawa, T., Okamoto, M., Kushiro, T., Nambara, E., Oono, Y., Seki, M., Kobayashi, M., Koshiba, T., Kamiya, Y., Shinozaki, K.
  • Journal Title: Plant J. (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Abscisic acid and stress treatment are essential for the acquisition of embryogenic competence by carrot somatic cells. (2006)
  • Author(s): Kikuchi, A., Sanuki, N., Higashi, K., Koshiba, T., Kamada, H.
  • Journal Title: Planta (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesised in the endosperm is involved in the induction of seed dormancy. (2006)
  • Author(s): Lefebvre, V., North, H., Frey, A., Sotta, B., Seo, M., Okamoto, M., Nambara, E., Marion-Poll, A.
  • Journal Title: Plant J. 45 Pages: 309-319
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aldehyde oxidase (AO) in the root nodules of Luponus albus and Medicago truncatula : Identification of AO in meristematic and infection zones. (2005)
  • Author(s): Fedorova, E., Redondo, F.J., Koshiba, T., Pueyo, J.J., di Felipe, M.R., Lucas,M.M.
  • Journal Title: Mol.Plant Microbe Interact. 18 Pages: 405-413
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Genome-wide profiling of stored mRNA in Arabidopsis thalianaseed germination : eigenetic and genetic regulation of transcription in seed. (2005)
  • Author(s): Nakabayashi, K., Okamoto, M., Koshiba, T., Kamiya, Y., Nmbara,E.
  • Journal Title: plant Journal 41 Pages: 697-709
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Possible involvement of abscisic acid in the induction of secondary somatic embryogenesis on seed coat-derived carrot somatic embryos. (2005)
  • Author(s): Ogata, Y., Iizuka, M., Nkayama, D., Ikeda, M., Kamada, H., KOshiba,T.
  • Journal Title: Planta 221 Pages: 417-423
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cold acclimation in bryophytes : Low temperature-induced freezing tolerance accompanied by increases in expression of stress-related genes but not those in endogenous abscicaid. (2005)
  • Author(s): Minami, A., Nagao, M., Ikegami, K, Koshiba, T., Arakawa, K., Fujikawa, S., Takezawa,D.
  • Journal Title: planta 220 Pages: 414-423
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Aldehyde oxidase (AO) in the root nodules of Lupinus albus and Medicago truncatula : Identification of AO in meristematic and infection zones. (2005)
  • Author(s): Fedrova, E., Redondo, F.J., Koshiba, T., Pueyo, J.J., de Felipe, M.R., Lucas, M.M.
  • Journal Title: Mol.Plant Microbe Interact 18 Pages: 405-413
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination : epigenetic and genetic regulation of transcription in seed. (2005)
  • Author(s): Nakabayashi, K., Okamoto, M., Koshiba, T., Kamiya, Y., Nambara, E.
  • Journal Title: Plant J. 41 Pages: 697-709
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Possible involvement of abscisic acid in the induction of secondary somatic embryogenesis on seed coat-derived carrot somatic embryos. (2005)
  • Author(s): Ogata, Y., Iizuka, M., Nakayama, D., Ikeda, M., Kamada, H., Koshiba, T.
  • Journal Title: Planta 221 Pages: 417-423
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cold acclimation in biyophytes : Low temperature-induced freezing tolerance accompanied by increases in expression of stress-related genes but not those in endogenous abscisic acid. (2005)
  • Author(s): Minami, A., Nagao, M., Ikegami, K., Koshiba, T., Arakawa, K., Fujikawa, S., Takezawa, D.
  • Journal Title: Planta 220 Pages: 414-423
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Comparative studies on the Arabidopsis aldehyde oxidase (AAO) gene family revealed a major role of AA03 in ABA biosynthesis in seeds. (2004)
  • Author(s): Seo, M., Aoki, H., Koiwai, H., Kmiya, Y., Nambara, E., Koshiba,T.
  • Journal Title: Plant Cell Physiol. 45 Pages: 1694-1703
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Actiyity and protein level of AO isoforms in pea plants(Pisumsativum L.)during vegetative development and in response to stress conditions. (2004)
  • Author(s): zdunek-Zastocka, E., Omarov, R.T., Koshiba, T., Lips, H.S.
  • Journal Title: J.Exp.Bot. 55 Pages: 1361-1369
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Tissue-specific localization of an abscisic acid biosynthetic emzyme,AA03,in Arabidopsis. (2004)
  • Author(s): Koiwai, H, Nakaminami, K., Seo, M., Mitsuhashi, W, Toyomasu, T, Olshiba,T.
  • Journal Title: Plant Physiol. 134 Pages: 1697-1707
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] The Arabidopsis cytochrome P450 CYP707A encodes ABA8'-hydroxylases : key enzymes in ABA catabokism. (2004)
  • Author(s): Kushiro, T., Okamoto, M., Nakabayashi, K., Yamagishi, K., Kitamura, S., Asami, T., Hiral, N., Koshiba, T., kamiya, T., Nambara,E.
  • Journal Title: EMBO J. 23 Pages: 1647-1656
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Comparative studies on the Arabidopsis aldehyde oxidase (AAO) gene family revealed a major role of AAO3 in ABA biosynthesis in seeds. (2004)
  • Author(s): Seo, M., Aoki, H., Koiwai, H., Kamiya, Y., Nambara, E., Koshiba, T.
  • Journal Title: Plant Cell Physiol 45 Pages: 1694-1703
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Activity and protein level of AO isoforms in pea plants (Pisurm sativum L.) during vegetative development and in response to stress conditions. (2004)
  • Author(s): Zdunek-Zastocka, E., Omarov, R.T., Koshiba, T., Lips, H.S.
  • Journal Title: J.Exp.Bot. 55 Pages: 1361-1369
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Tissue-specific localization of an abscisic acid biosynthetic enzyme, AAO3, in Arabidopsis. (2004)
  • Author(s): Koiwai, H, Nakaminami, K., Seo, M, Mitsuhashi, W, Toyomasu, T, Kosiba, T.
  • Journal Title: Plant Physiol. 134 Pages: 1697-1707
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases : key enzymes in ABA catabolism. (2004)
  • Author(s): Kushiro, T., Okamoto, M., Nakabayashi, K., Yamagishi, K., Kitamura, S., Asami, T., Hirai, N., Koshiba, T., Kamiya, Y., Nambara, E.
  • Journal Title: EMBO J. 23 Pages: 1647-1656
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] CYP707A3 amajor ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana.
  • Author(s): Umezawa, T., Okamoto, M., Kushiro, T., Nambara, E., Ono, Y., Seki, M., Kobayashi, M., Koshiba, T., Kamiya, Y., shinozaki,K.
  • Journal Title: Plant Journal 2006(印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Abscisic acid and stress treatment are essential for acquisition of embryogenic competence by carrot somatic cells.
  • Author(s): Kikushi, A., Sanuki, N, Higashi, K., Koshiba, T., Kamada,H.
  • Journal Title: Planta 2006(印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 植物ホルモンの分子細胞生物学 (2006)
  • Author(s): 小柴共一
  • Publisher: 植物の生長調節
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 植物ホルモンの分子細胞生物学 (2006)
  • Author(s): 小柴共一, 神谷勇治
  • Publisher: 講談社
  • Description: 「研究成果報告書概要(和文)」より
• [Book] 植物ホルモンの分子細胞生物学(小柴共一・神谷勇治/編) (2006)
  • Author(s): 小柴共一(分担)
  • Publisher: 講談社
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] ストレス応答性遺伝子が導入された形質転換植物 (2006)
  • Inventor(s): 小柴共一, 寺川輝彦, 長谷川久和, 小松節子, 岡本龍史, 古川聡子, 島谷健太郎
  • Industrial Property Rights Holder: 首都大学, 東京, 独法農業生物資源研, 北與化学(株)
  • Industrial Property Number: 国内特願2006-018661号
  • Filing Date: 2006-01-27
  • Description: 「研究成果報告書概要(和文)」より