2006 Fiscal Year Final Research Report Summary
Comprehensive analysis of protein interactions for determination of protein function in rice
| Project/Area Number |
16380010
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | YOKOHAMA CITY UNIVERSITY |
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Principal Investigator |
HIRANO Hisashi YOKOHAMA CITY UNIVERSITY, International Graduate School of Arts and Sciences, Professor, 大学院国際総合科学研究科生体超分子科学専攻, 教授 (00275075)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASAKI Hiroshi YOKOHAMA CITY UNIVERSITY, International Graduate School of Arts and Sciences, Associate Professor, 国際総合科学研究科, 准教授 (70169704)
SASSA Hidenori Chiba University, Department of Horticulture, Associate Professor, 園芸学部, 准教授 (50295507)
TANGA Michifumi Toyo Kohan Co., Director, 部長
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| Project Period (FY) |
2004 – 2006
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| Keywords | proteome / protein interaction / DLC plate / protein chip / glycoprotein / rice / soybean |
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| Research Abstract |
Protein chip has been one of the most promising tools for identification of expressed proteins and study of protein-protein interaction in proteome analysis. We developed a novel protein chip plate, which is made of diamond-like carbon-coated stainless steel (DLC), and modified with N-hydroxysuccinimide ester on the surface of plate. Proteins separated by SDS gel electrophoresis or two-dimensional electrophoresis (2-DE) were successfully electroblotted and immobilized covalently onto the DLC plate to produce easily and rapidly a high-density protein chip. High blotting efficiency (25-70 %) of proteins from the gels onto the DLC plate was obtained by improvement of electrophoresis and electroblotting techniques. Using the DLC plate, we established the techniques for identification of proteins immobilized on the DLC plate, for detection of proteins or peptides interacted with proteins immobilized on the plate using matrix-assisted laser desorption ionization/time-of-flight mass spectrometry, and also we developed the technique for identification of post-translational modification of proteins on the plate. In this study, we applied these techniques to identification of a number of proteins and N-linked glycoproteins of the rice calli and soybean seeds, which were separated by 2-DE. The developed techniques have a great potential to make possible the high-throughput proteome analysis in plants.
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Research Products
(51 results)
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[Journal Article] On-chip identification and interaction analysis of gel-resolved proteins using a diamond-like carbon-coated plate.2007
Author(s)
Iwafune, Y., Tan, J.Z., Ino, Y., Okayama, A., Ishigaki, Y., Saito, K., Suzuki, N., Arima, M., Oba, M., Kamei, S., Tanga, M., Okada, T., Hirano, H.
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Journal Title
J. Proteome Res. 6
Pages: 2315-2322
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Slocus F-box brothers : Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear.2007
Author(s)
Sassa, H., Kakui, H., Miyamoto, M., Suzuki, Y., Hanada, T., Ushijima, K., Kusaba, M., Hirano, H., Koba, T.
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Journal Title
Genetics 175
Pages: 1869-1881
Description
「研究成果報告書概要(和文)」より
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[Journal Article] On-chip identification and interaction analysis of gel-resolved proteins using a diamond-like carbon-coated plate.2007
Author(s)
Iwafune, Y., Tan, J.Z.Ino, Y., Okayama, A., Ishigaki, Y, Saito, K., Suzuki, N., Arima, M., Oba, M., Kamei, S., Tanga, M., Okada, T., Hirano, H.
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Journal Title
J.Proteome Res. 6
Pages: 2315-2322
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] S locus F-box brothers : Multiple and pollen-specific F-box genes with S haplotype-specific polymorphisms in apple and Japanese pear.2007
Author(s)
Sassa, H., Kakui, H., Miyamoto, M., Suzuki, Y, Hanada, T., Ushijima, K., Kusaba, M., Hirano, H., Koba, T.
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Journal Title
Genetics 175
Pages: 1869-1881
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The analysis of N-glycolilneuraminic acid (NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry.2006
Author(s)
Asakawa, H., Sasabe, M., Miyazaki, R., Matsuda, H., Fukai, F., Hanada, K., Hirano, H., Takasaki, S.
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Journal Title
Proc. Japan Acad. Sci. Ser. B, 82
Pages: 181-187
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Efficient electroblotting of gel-resolved proteins onto diamond-like carbon-coated plate for protein-chip.2006
Author(s)
Ino, Y., Okayama, A., Iwafune, Y., Arakawa, N., Kikuchi, J., Kamita, M., Kawasaki, H., Okada, T., Hirano, H.
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Journal Title
J. Electrophoresis 53
Pages: 33-37
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Proteomic search for potential diagnostic markers and therapeutic targets for ovarian clear cell adenocarcinoma.2006
Author(s)
Morita, A., Miyagi, E., Yasumitsu, E., Kawasaki, H., Hirano, H., Hirahara, F.
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Journal Title
Proteomics 6
Pages: 5880-5890
Description
「研究成果報告書概要(和文)」より
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[Journal Article] A novel approach and protocol for discovering extremely low-abundance proteins in serum.2006
Author(s)
Tanaka, Y., Akiyama.H., Kuroda.T., Jung, G., Tanahashi, K., Sugaya, H., Utsumi, J., Kawasaki, H., Hirano, H.
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Journal Title
Proteomics 6
Pages: 4845-4855
Description
「研究成果報告書概要(和文)」より
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[Journal Article] 4-kDa peptide.2006
Author(s)
Hirano, H
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Journal Title
Handbook of Biological Active Peptides (Kastin, A.J. ed.)(Academic Press, London)
Pages: 1-4
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The analysis of N-glycolilneuraminic acid (NeuGc) of hepatoma tissue and K562 cell ferritins using HPLC and mass spectrometry.2006
Author(s)
Asakawa, H., Sasabe, M., Miyazaki, R., Matsuda, H., Fukai, F., Hanada, K., Hirano, H., Takasaki, S.
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Journal Title
Proc.Japan Acad.Sci.Ser.B 82
Pages: 181-187
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Efficient electroblotting of gel-resolved proteins onto diamond-like carbon-coated plate for protein-chip.2006
Author(s)
Ino, Y., Okayama, A., Iwafune, Y, Arakawa, N., Kikuchi, J., Kamita, M., Kawasaki, H., Okada, T., Hirano, H.
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Journal Title
J.Electrophoresis 53
Pages: 33-37
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Proteomic search for potential diagnostic markers and therapeutic targets for ovarian clear cell adenocarcinoma.2006
Author(s)
Morita, A.Miyagi, E., Yasumitsu, E., Kawasaki, H., Hirano, H., Hirahara, F.
-
Journal Title
Proteomics 6
Pages: 5880-5890
Description
「研究成果報告書概要(欧文)」より
-
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[Journal Article] A novel approach and protocol for discovering extremely low-abundance proteins in serum.2006
Author(s)
Tanaka, Y, Akiyama.H., Kuroda.T., Jung, G, Tanahashi, K., Sugaya, H., Utsumi, J., Kawasaki, H., Hirano, H.
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Journal Title
Proteomics 6
Pages: 4845-4855
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Protein extraction from mature rice leaves for two-dimensional gel electrophoresis and its application in proteome analysis.2004
Author(s)
Islam, N., Lonsdale, M., Upadhyaya, N.M., Higgins, T.J., Hirano, H., Akhurst, R.
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Journal Title
Proteomics 4
Pages: 1903-1908
Description
「研究成果報告書概要(和文)」より
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