• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

The role of singlet oxygen in ischemia-reperfusin injury : the study using laser scanning confocal microscopy and electron paramagnetic resinance

Research Project

Project/Area Number 16390450
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

ARAI Toshiyuki  Kyoto University, Medicine, Associated Professor, 医学研究科, 助教授 (80175950)

Co-Investigator(Kenkyū-buntansha) ENDO Nobuyuki  Wakasa Wan Energy Research Center, Biology, Researcher, 研究部, 研究員 (30359244)
Project Period (FY) 2004 – 2005
Keywordsischemia-reperfusion injury / singlet oxygen / 6-formylpterin / photodynamic effects / rat hepatocytes / human pancreatic cancer cell line / human leukemia cell line / human dendritic cells
Research Abstract

To elucidate the role of singlet oxygen in ischemia-reperfusion injury, the following studies were performed.
1)The generation and quenching of singlet oxygen were chemically examined. When the solution including 6-formylpterin (6FP) was irradiated by fluorescent llight, singlet oxygen was generated, which was quenched by N-acetyl-L-cystein (NAC), glutathine (GSH) and histidine.
2)The biological effects of photochemically generated singlet oxygen against were examined using rat hepatocytes. When the hepatocyted were loaded with 6FP and then irradiated by fluorescent llight, the cell death was induced, which was attenuated by the pre-treatment with NAC and GSH, but not with histidine.
3)The effects of reactive oxygen species (ROS) generated by 6FP on the drug-induced cell injury were examined. When the rat hepatocytes were loaded with tumor necrosis factor-α and actinomycin D, the apoptotic cell injury was induced, which was attenuated by the pre-treatment with 6FP. The generated ROS by 6FP was thought to suppress the caspase activity and attenuated the apoptotic cell injury.
4)The photodynamic effects of a novel pterin derivative were examined using a human pancreatic cancer cell line, Panc-1 cells. When the cells were loaded with the novel pterin and irradiated by ultraviolet (UV-A), the cell death was induced. The singlet oxygen generated during irradiation was thought to cause the cell death.
5)The effects of 6FP on the heat-induced apoptosis were examined using a human leukemia cell line, U937 cells. When the cells were loaded with 6FP and exposed to heat shock (44℃ for 20 min), the apoptotic cell death was enhanced. The hydrogen peroxide generated by 6FP was thought to cause the enhancement.
6)Lipopolysaccharide (LPS)-induced ROS generation and changes in glutathione level and their relation to the maturation of dendritic cells were examined. It was revealed that the ROS was involved in the cytokine production.

  • Research Products

    (12 results)

All 2006 2005

All Journal Article (12 results)

  • [Journal Article] LPS-induced ROS generation and changes in glutathione level and their relation to the maturation of human monocyte-derived dendritic cells2006

    • Author(s)
      Yamada H, Arai T, Endo N, et al.
    • Journal Title

      Life Sciences 78

      Pages: 926-933

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Novel 6-formylpterin derivatives : chemical synthesis and O_2 to ROS conversion activities2006

    • Author(s)
      Nonogawa M, Arai T, Endo N, et al.
    • Journal Title

      Org Biomol Chem 4

      Pages: 1811-1816

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation of hypoxia-inducible factor 1 during macrophage differentiation2006

    • Author(s)
      Oda T, Hirota K, Nishi K, et al.
    • Journal Title

      Am J Physiol Cell Physiol (発表予定)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] LPS-induced ROS generation and changes in glutsthione level and their relation to the maturation of human monocyte-derived dendritic cells.2006

    • Author(s)
      Yamada H, Arai T, Endo N, Yamashita K, Fukuda K, Sasada M, Uchiyama T
    • Journal Title

      Life Sciences 78

      Pages: 926-933

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Novel 6-formylpterin derivatives : chemical synthesis and O_2 to ROS conversion activities.2006

    • Author(s)
      Nonogawa M, Arai t, Endo N, Pack S-P, Kodaki T, Makino K
    • Journal Title

      Org Biomol Chem 4

      Pages: 1811-1816

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Activation of hypoxia-inducible factor 1 during macrophage differentiation.2006

    • Author(s)
      Oda T, Hirota K, Nishi K, Takabuchi S, Oda S, Yamada H, Arai T, Fukuda K, Kita T, Adachi T, Semenza GL, Nohara R
    • Journal Title

      Am J Physiol Cell Physiol (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Protective effects of intracellular reactive oxygen species generated by 6-formylpterin tumor necrosis factor-α-induced apoptotic cell injury in cultured rat hepatocytes2005

    • Author(s)
      Ishii H, Arai T, Mori H, et al.
    • Journal Title

      Life Sciences 77

      Pages: 858-868

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Photodynamic effects of a novel pterin derivative on a pancreatic cancer cell line2005

    • Author(s)
      Yamada H, Arai T, Endo N, et al.
    • Journal Title

      Biochem Biophys Res Commun 333

      Pages: 763-767

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A hydrogen peroxide-generating agent, 6-formylpterin, enhances heat-induced apoptosis2005

    • Author(s)
      Wada S, Cui Z-G, Kondo T, et al.
    • Journal Title

      Int J Hyperthermia 21(3)

      Pages: 231-246

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Protective effects of intracellular reactive oxygen species generated by 6-formylpterin on tumor necrosis factor-α-induced apoptotic cell injury in cultured rat hepatocytes.2005

    • Author(s)
      Ishii H, Arai T, Mori H, Yamada H, Endo N, Makino K, Fukuda K
    • Journal Title

      Life Sci 77

      Pages: 858-868

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Photodynamic effects of a noel pterin derivative on a pancreatic cancer cell line.2005

    • Author(s)
      Yamada H, Arai T, Endo N, Yamashita K, Nonogawa M, Makino K, Fukuda K, Sasada M, Uchiyama T
    • Journal Title

      Biochem Biophys Res Commun 333

      Pages: 763-767

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A hydrogen peroxide-generating agent, 6-formylpterin, enhances heat-induced apoptosis.2005

    • Author(s)
      Wada S, Cui Z-G, Kondo T, Zhao Q-L, Ogawa R, Shoji M, Arai T, Makino K, Furuta I
    • Journal Title

      Int J hyperthermia 21

      Pages: 231-246

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi