Relationship between Hsp47 and cornification mechanisms of oral mucosa keratinocytes after transplantation of cultured gingival fibroblasts

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16390595
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University.
• Principal Investigator: INOKUCHI Tsugio Nagasaki University, Graduate school of Biomedical Sciences, Department of Developmental and Reconstructive Medicine, Professor, 大学院医歯薬学総合研究科, 教授 (40136685)
• Co-Investigator(Kenkyū-buntansha): NEMOTO Takayuki Nagasaki University, Graduate school of Biomedical Sciences, Department of Developmental and Reconstructive Medicine, Professor, 大学院医歯薬学総合研究科, 教授 (90164665) ; OHBA Seigo Nagasaki University, Graduate school of Biomedical Sciences, Department of Developmental and Reconstructive Medicine, Assistant professor, 医学部・歯学部附属病院, 助手 (80363456)
• Project Period (FY): 2004 – 2005
• Keywords: Hsp47 / fibroblast / oral mucosa kerainocyte / in vitro

Research Abstract

We took palatal mucosa and vestibule mucosa of SD rat and got fibroblasts with outgrowth method. Fibroblast, originated from attached gingiva, was injected under buccal mucosa. Accordingly buccal mucosa was reacted and turned its behavior to keratinized mucosa. But a part of mucosa formed like a scar tissue. We think this phenomenon will contribute for the treatment of enhancing attached gingiva for the dental implant. Now we are examining the method of attached gingival acquisition without scar formation.
We are using fibroblast originated from fetal palatal mucosa and injected in the free gingiva of Nude Rat. This concept derives from the fact that fatal wound repair proceeds without scar formation. In addition we examined the expression of Hsp47 and collagen in the morphological transformation mucosa using immunohistochemical and molecular biological methods, the amount of both proteins increased around the place of injected fibroblast originated from attached gingiva but some area formed scar tissue.
We are also under consideration about exchanges of information between periosteum and cell transplantation.