2005 Fiscal Year Final Research Report Summary
Investigation on Molecular Mechanism of Sucrose-degrading Enzyme in Asian Honeybee.
| Project/Area Number |
16405025
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied biochemistry
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KIMURA Atsuo Hokkaido Univ., Grad.School of Agr., Prof., 大学院・農学研究科, 教授 (90186312)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Haruhide Hokkaido Univ., Grad.School of Agr., Asso.Prof., 大学院・農学研究科, 助教授 (80241363)
OKUYAMA Masayuki Hokkaido Univ., Grad.School of Agr., Assi.Prof., 大学院・農学研究科, 助手 (00344490)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Asian honeybee / α-glucosidase / honey |
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| Research Abstract |
We have analyzed three sucrose-degrading isozymes (α-glucosidase I,II, and III) in European honeybees, since these enzymes exhibited the interesting activity to sucrose of high concentration. α-Glucosidase I was a unique allosteric enzyme to display negative cooperativity in extremely high sucrose concentration. α-Glucosidase II also exhibited the allosteric property of positive cooperativity. α-Glucosidase III was a Michaelis-Menten-type enzyme to be secreted to nectar (bees gathered from flower) and to contribute to the formation of honey. Purpose of this project is i)existence of allosteric α-glucosidase in Asian honeybees, ii)investigation of honey-forming mechanism, and iii)regulation in expression of three α-glucosidase isozymes.
Four kinds of honeybee species were investigated in this research. From typical Asian honeybees (living in Thailand, Korea and Japan), three isozymes were isolated by chromatographic method. One of them was an allosteric enzyme having similar properties t
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o European honeybee α-glucosidase I, allowing to molecular analysis of negative cooperativity. The second enzyme was α-glucosidase II-type isozyme. Enzyme in honey was also investigated, and showed similar to one of enzymes (α-glucosidase III-type isozyme). cDNAs of these three enzyme were cloned. It was found that the expression of three isozymes was regulated. In small honeybees living in Thailand, three α-glucosidases were also found, in which two α-glucosidases were isolated. Their properties investigated were almost identical to those of α-glucosidases I and II. Enzyme in honey was compared with adult bee enzymes. All honeybee species studied in this project contained allosteric enzyme. Asian honeybees were divided into two types : i)having three α-glucosidase isozymes and ii)having two α-glucosidase isozymes. Recently, we found that bumblebees also had two α-glucosidase isozymes. Further research will elucidate the interesting molecular mechanism of sucrose-degrading enzymes in flower bees. Less
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Research Products
(38 results)
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[Journal Article] Indentification of amino acids residues for key role in dextransucrase activity of Leuconostoc mesenteroides B-742CB.2004
Author(s)
Ryu HJ, Kim D, Seo ES, Kang HK, Lee JH, Yoon SH, Cho JY, Robyt JF, Kim DW, Chang SS, Kim SU, Kimura A
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Journal Title
J Microbiol Biotechnol 14-5
Pages: 1075-1080
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Localization of α-glucosidases I,II, and III in organs of European honeybee, Apis mellifera L., and origin of α-glucosidase in honey.2004
Author(s)
Kubota M, Tsuji M, Nishimoto M, Wongchawalit J, Okuyama M, Mori H, Masui H, Surarit R, Svasti J, Kimura A, Chiba S
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Journal Title
Biosci Biotechnol Biochem 68-11
Pages: 2346-2352
Description
「研究成果報告書概要(欧文)」より
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