2005 Fiscal Year Final Research Report Summary
Development of a novel strategy for mutation-specific cloning using DNA repair enzyme
| Project/Area Number |
16510149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Kitasato University |
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Principal Investigator |
TSUKAMOTO Toshihiko Kitasato University, School of Pharmaceutical Sciences, Associate Professor, 薬学部, 助教授 (10236862)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Mutation |
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| Research Abstract |
In this study, we pulled down heteroduplexes formed between cDNA libraries constructed from a colorectal cancer with high microsatellie instability (MSI-H) and paired normal mucosa using a recombinant MutS protein and isolated paires of mutant and wild type sequences involving ; CEA (carcinoembryonic antigen-related cell adhesion molecule 5 ; Hs.220529) 3'UTR, 2780 A to G ; 2792-2798 (T) 7 to (T) 8 (GI : 178676 ; CDS 97..2205) ; CCNI (Cyclin I ; Hs.486360) 3' UTR, 1800 (A) 10 to (A) 9 (GI : 13436391 ; CDS 544..1677) ; PTMA (prothymosin, alpha ; Hs.264317) 5'UTR, 142 T to C ; CDS, 228 T to C (GI : 47938172 ; CDS 161..493) ; SRI (sorcin ; Hs.489040) CDS 488 A->G, Y159C (GI : 4507206 ; CDS 13..609) ; LGALS3 (lectin, galactoside-binding, soluble, 3 ; galectin 3 ; Hs.531081) 774 A to G, 1250M (GI : 37589086 ; CDS 25..777) ; RACK1 (GNB2L1, guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 ; Hs.5662) 288t->g, F65V, 304t->c, V70A (GI : 187701 ; CDS 96..1049) ; UQCRQ (ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa ; Hs.146602) CDS 227 t->C, F67S, (GI:34784784 ; CDS 28..276) ; and also isolated a novel NAT highly mutated in MSI-H tumors for thymidylate synthase.
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Research Products
(8 results)
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[Journal Article] cDNA array analysis for diagnosis of hepatic metastasis of colorectal carcinoma using tissue samples obtained from primary tumors.
Author(s)
Takata, O., Kawamura, Y., Konishi, F., Sasaki, J., Kai, T., Miyakura, Y., Nagai, H., Tsukamoto, T.
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Overexpression in colorectal cancer of two lysosomal enzymes, CLN2 and CLN1, involved in neuronal ceroid lipofuscinosis.
Author(s)
Tsukamoto, T., Iida, J., Dobashi, Y., Furukawa, T., Konishi, F.
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Clinicopathological characteristics of colorectal cancers with loss of imprinting of insulin-like growth factor 2.
Author(s)
Sasaki, J., Konishi, F., Kawamura, Y, Kai., T., Takata, O., Tsukamoto, T.
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Journal Title
Description
「研究成果報告書概要(和文)」より
-
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[Journal Article] cDNA array analysis for diagnosis of hepatic metastasis of colorectal carcinoma using tissue samples obtained from primary tumors.
Author(s)
Takata, O., Kawamura, Y., Konishi, F., Sasaki, J., Kai, T., Miyakura, Y., Nagai, H., Tsukamoto, T.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Overexpression in colorectal cancer of two lysosomal enzymes, CLN2 and CLN1, involved in neuronal ceroid lipofuscinosis.
Author(s)
Tsukamoto, T., Iida, J., Dobashi, Y., Furukawa, T., Konishi, F.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
-
[Journal Article] Clinicopathological characteristics of colorectal cancers with loss of imprinting of insulin-like growth factor 2.
Author(s)
Sasaki, J., Konishi, F., Kawamura, Y, Kai., T., Takata, O., Tsukamoto, T.
-
Journal Title
Description
「研究成果報告書概要(欧文)」より
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