2006 Fiscal Year Final Research Report Summary
Signal transduction pathways leading to Ca2+ release in fertilization: focusing on egg membrane-associated protein complexes
| Project/Area Number |
16570174
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Kobe University |
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Principal Investigator |
SATO Ken-ichi Kobe University, Research Center for Environmental Genomics, Assistant Professor, 遺伝子実験センター, 助手 (30235337)
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| Project Period (FY) |
2004 – 2006
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| Keywords | fertilization / signal transduction / membrane rafts / Src / egg activation / uroplakin III / gamete interaction / early development |
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| Research Abstract |
We have analyzed molecular mechanism of fertilization by using African clawed frog Xenopus laevis as a main model organism. By focusing on molecular identity of a 57-kDa protein-tyrosine kinase that is immunochemically related to the Src family kinases and the structure and function of the egg plasma membrane-associated protein complexes, we have found that Xenopus Src gene product (termed xSrc) and a 30-kDa transmembrane protein uroplakin III (termed xUPIII) are important factors for sperm-induced egg activation, namely transient Ca2+ release within the fertilized egg. xUPIII has been initially identified as a major tyrosine-phosphorylated protein in fertilized Xenopus eggs. Further analysis of xUPIII has demonstrated that it is a target of sperm-derived protease activity and that partial proteolysis of the extracellular domain of xUPIII at fertilization may act as a signal to promote sperm-induced xSrc activation. Because both xUPIII and Src localizes predominantly in cholesterol-enriched egg membrane microdomains or "rafts", it is assumed that protein complexes organized in the membrane microdomains serve as a functional platform for sperm-egg membrane interaction and subsequent Src-dependent calcium signaling at fertilization. We have also identified uroplakin Ib (termed xUPIb), as a binding partner for UPIII and expression studies using HEK293 cells show that co-expression of xUPIII and xUPIb is required for their proper localization to the membrane microdomain as well as maintenance of xSrc tyrosine kinase activity in an inactive state. These results highlights the scheme that sperm-induced egg activation in Xenopus involves molecular machinery that act for gamete interaction in the extracellular space and that translates the sperm signal into the intracellular space, both of which are localized in the egg membrane microdomains
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[Journal Article] Characterization of Xenopus egg membrane microdomains containing uroplakin Ib/III complex : roles of their molecular interactions for subcellular localization and signal transduction.2007
Author(s)
Mahbub Hasan, A.K.M., Ou, Z., Sakakibara, K., Hirahara, S., Iwasaki, T., Sato.K.Fukami, Y.
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Journal Title
Genes Cells 12
Pages: 251-267
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Activation of Arabidopsis MAPK kinase kinase (AtMEKK1) and induction of AtMEKK1-AtMEK12006
Author(s)
Hadiarto, T., Nanmori, T., Matsuoka, D., Iwasaki, T., Sato.K., Fukami, Y., Azuma, T., Yasuda, T.
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Journal Title
Planta 223
Pages: 708-713
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Phylogeny of vertebrate Src tyrosine kinase as revealed by the epitope region for mAb327.2006
Author(s)
Iwasaki, T., Sato.K., Yoshino, K., Itakura, S., Kosuge, K., Tokmakov, A.A., Owada, K., Yonezawa, K., Fukami, Y.
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Journal Title
J. Biochem. 139
Pages: 347-354
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Studying fertilization in cell free extracts : focusing on membrane/lipid raft functions and proteomics.2006
Author(s)
Sato.K., Yoshino, K., Tokmakov, A.A., Iwasaki, T., Yonezawa, K., Fukami, Y.
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Journal Title
Methods Mol Biol. 322
Pages: 395-411
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Regulation of Src kinase activity during Xenopus oocyte maturation.2005
Author(s)
Tokmakov, A.A., Iwasaki, T., Itakura, S., Sato.K..Shirouzu, M., Fukami, Y., Yokoyama, S.
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Journal Title
Dev. Biol. 278
Pages: 389-300
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Molecular identification and characterization of Xenopus egg uroplakin III, an egg raft-associated transmembrane protein that is tyrosine- phosphorylated upon fertilization.2005
Author(s)
Sakakibara, K., Sato.K., Yoshino, K., Oshiro, N., Hirahara, S., Hasan, A.K.M.M., Iwasaki, T., Ueda, Y., Iwao, Y., Yonezawa, K., Fukami, Y.
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Journal Title
J. Biol. Chem. 280
Pages: 15029-15037
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Functional, biochemical, and chromatographic characterization of the complete [Ca^<2+>] oscillation- inducing activity of porcine sperm.2005
Author(s)
Kurokawa, M., Sato.K., Wu, H., He, C., Malcuit, C., Black, S.J., Fukami, K., Fissore, R.A.
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Journal Title
Dev. Biol. 285
Pages: 376-392
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Uroplakin III, a novel Src substrate in Xenopus egg rafts, is a target for sperm protease essential for fertilization.2005
Author(s)
Mahbub Hasan, A.K.M., Sato.K..Sakakibara, K., Ou, Z., Iwasaki, T., Ueda, Y., Fukami, Y.
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Journal Title
Dev. Biol. 286
Pages: 483-492
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Evidence that activation of a Src family kinase is not required for fertilization-associated [Ca^<2+>] oscillations in mouse eggs.2004
Author(s)
Kurokawa, M., Sato.K..Smyth, J., Wu, H., Fukami, K., Takenawa, T., Fissore, R.A.
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Journal Title
Reproduction 127
Pages: 443-456
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fertilizome project : Study on the biological bridge between gametogenesis and embryogenesis.2004
Author(s)
Sato.K., Sette, C., Kurokawa, M., Paronetto, M.P., Iwasaki, T., Fissore, R.A., Fukami, Y.
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Journal Title
Current Proteomics 1
Pages: 231-246
Description
「研究成果報告書概要(欧文)」より
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