2005 Fiscal Year Final Research Report Summary
Research on the Aerial cell-surface Localization of Hydrophobins
| Project/Area Number |
16580052
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Meiji Universituy |
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Principal Investigator |
NAKAJIMA Harushi Meiji University, Dept.of Agricultural Chemistry, Associate Professor, 農学部, 助教授 (10217721)
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| Project Period (FY) |
2004 – 2005
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| Keywords | hydrophobin / hypA / Aspergillus oryzae / solid-state culture / cell surface engineering |
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| Research Abstract |
Hydrophobins are small amphipathic proteins found uniquely in filamentous fungi that show surface-activity and self-assembly. To investigate the localization of hydrophobin A (HypA) in Aspergillus oryzae, hypA-egfp fusion gene was constructed and introduced into A.oryzae. By fluorescent microscopy, the fluorescence was specifically localized on surface of conidia in solid-state culture. In submerged cultures, the fluorescence was hardly observed in the hyphae. By western blot analysis using anti-GFP antibody, HypA-eGFP fusion was detected in the solid-state culture and medium of submerged culture. We also observed, an additional high molecular weight band, which was considered to be self-assembled HypA-eGFP, in the solid-state culture. This observation indicates that EGFP-tagged HypA was correctly localized and assembled on the surface of conidia.
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