2006 Fiscal Year Final Research Report Summary
Improvement in ozone tolerance of poplar plants with an antisense and sense DNA for 1-aminocyclopropane-1-carboxylate synthase
| Project/Area Number |
16580125
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
林学・森林工学
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
MOHRI Takeshi Forestry and Forest Products Research Institute, Department of Molecular and cell Biology, Senior Researcher, 生物工学研究領域, 主任研究員 (40353883)
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| Project Period (FY) |
2004 – 2006
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| Keywords | transgenic plants / ethylene / ozone / poplar |
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| Research Abstract |
Antisense and sense DNA for an ozone-inducible 1-aminocyclopropane-1-carboxylate (ACC) synthase from poplar, under the control of the cauliflower mosaic virus 35S promoter, was introduced into poplar to generate transgenic plants. Lower rates of ozone-induced ethylene production were observed in fifteen of twenty transgenic plants than in the wild-type plants. Ozone-induced visible damage was attenuated in these fifteen lines, and the extent of damage was positively related to the level of ozone-induced ethylene production. On the other hand higher rates of ozone-induced ethylene production were observed in four of ten transgenic plants than in the wild-type plants. No significant differences in growth and morphology were observed between transgenic and wild-type plants. Stomatal conductance of transgenic plants during ozone exposure was higher than that of wild-type plants. These findings indicate that the introduction of antisense and sense DNA for an ozone-inducible ACC synthase can improve the ozone tolerance of plants without reducing their gas absorption and productivity.
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