Development of a novel method to conserve genetic information of fish by cryopreservation of primordial germ cells

2005 Fiscal Year Final Research Report Summary

• Project/Area Number: 16580149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: YOSHIZAKI Goro Tokyo University of Marine Science and Technology, Department of Marine Biosciences, Associate professor, 海洋科学部, 助教授 (70281003)
• Project Period (FY): 2004 – 2005
• Keywords: cryopreservation / primordial germ cell / surrogate broodstock technology / germ cell transplantation / vasa / GFP / genetic resources / teleost

Research Abstract

A method for cryopreserving fish embryos and eggs has previously been lacking. In this study, we have regenerated live offspring from cryopreserved materials for the first time in lower vertebrates. Our novel method allows the successful cryopreservation of rainbow trout primordial germ cells (PGCs). Moreover, through transplantation into allogenic recipients, the cryopreserved PGCs can develop into functional eggs and produce viable fry upon fertilization with cryopreserved spermatozoa. PGC cryopreservation and transplantation will allow the conservation of genetic information from endangered fish species. Our system will also be a powerful tool for maintaining domesticated fish strains carrying commercially valuable traits.

Research Products

• [Journal Article] Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional eggs in fish. (2006)
  • Author(s): Okutsu T, Suzuki K, Takeuchi Y, Takeuchi T, Yoshizaki G.
  • Journal Title: Proc Natl Acad Sci USA. 103 Pages: 2725-2729
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional eggs in fish. (2006)
  • Author(s): Okutsu T, Suzuki K, Takeuchi Y, Takeuchi T, Yoshizaki G
  • Journal Title: Proc Natl Acad Sci USA. 103 Pages: 2725-2729
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] GFP-labeling of primordial germ cells using a non-transgenic method and its application for germ cell transplantation in salmonidae. (2005)
  • Author(s): Yoshizaki, G., Tago, Y., Takeuchi, Y., Sawatari, E., Kobayashi, T., Takeuchi, T.
  • Journal Title: Biology of Reproduction 73 Pages: 88-93
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] GFP-labeling of primordial germ cells using a non-transgenic method and its application for germ cell transplantation in salmonidae. (2005)
  • Author(s): Yoshizaki, G., Tago, Y., Takeuchi, Y., Sawatari, E., Kobayashi, T., Takeuchi, T.
  • Journal Title: Biology of Reproduction, 73 Pages: 88-93
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Surrogate brood stock produces salmonids. (2004)
  • Author(s): Takeuchi, Y., Yoshizaki, G., Takeuchi, T.
  • Journal Title: Nature 430 Pages: 629-630
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Surrogate broodstock produces salmonids. (2004)
  • Author(s): Takeuchi, Y., Yoshizaki, G., Takeuchi, T.
  • Journal Title: Nature 430 Pages: 629-230
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Generation of viable fish from cryopreserved primordial germ cells.
  • Author(s): Kobayashi T, Takeuchi Y, Takeuchi T, Yoshizaki G.
  • Journal Title: Molecular Reproduction and Development (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Generation of viable fish from cryopreserved primordial germ cells
  • Author(s): Kobayashi T, Takeuchi Y, Takeuchi T, Yoshizaki G.
  • Journal Title: Molecular Reproduction and Development (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] サケからマスをつくる"始原生殖細胞を用いた魚類の発生工学"、クローンとエピジェネティクスの新展開(今川和彦 編) (2006)
  • Author(s): 吉崎悟郎
  • Total Pages: 21
  • Publisher: アドスリー
  • Description: 「研究成果報告書概要(和文)」より