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2005 Fiscal Year Final Research Report Summary

Study on easy diagnostic system for cervical cancer with using p16 antibody

Research Project

Project/Area Number 16590267
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionGunma University

Principal Investigator

SANO Takaaki  GUNMA UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, LECTURER, 大学院・医学系研究科, 講師 (90292581)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Tomomi  GUNMA UNIVERSITY, FACULTY OF MEDICINE, RESEARCH ASSISTANT, 医学部, 助手 (00312893)
Project Period (FY) 2004 – 2005
Keywordsp16 / cervical cancer / uterine corpus cancer / 14-3-3sigma / HPV / P14ARF / thinlayer cytological sample / liquid based cytology
Research Abstract

In cervical lesions, the overexpression of p16 is reported to be closely associated with high-risk human papillomavirus infection (Am J Pathol, 1998). The objective of the current study was to confirm the usefulness of liquid-based cervical specimens for p16 staining wa well as tissue sections. The results of the study confirmed that immunohistochemical detection of p16 was more sensitive and specific than HPV status in cervical lesions using a liquid based method, suggesting that p16 should be used as a satisfactory biomarker for the primary screening of cervical cytology (Cancer cytopathol, 2004).
14-3-3sigma has been a major G2/M checkpoint control gene. In order to confirm 14-3-3sigma protein expression together with p16 in cervical cancers, immunohistochemistry was performed. Strong and diffuse immunoreactivity for 14-3-3sigma was uniformly observed in all cervical dysplasia and carcinomas including adenocarcinomas. The results of the study confirmed that the majority of cervical cancers with p16 overexpression also showed overexpression of 14-3-3sigma (Pathol Int, 2004).
We also investigated the expression of 14-3-3sigma in endometrial adenocarcinoma and non-neoplastic endometria, both which might be obtained together with cervical lesions by cytology specimens. In normal endometrium, 14-3-3sigma was overexpressed in the mid- to late- secretory phase. In endometrial adenocarcinoma, 14-3-3sigma expression increased significantly with increasing histological grade, myometrial invasion. These results suggest that careful attention is needed on the interpretation for immunohistochemical expression of 14-3-3sigma in cervical specimens including normal or neoplastic endometria (Pathol Int, 2005).

  • Research Products

    (7 results)

All 2005 2004

All Journal Article (7 results)

  • [Journal Article] Increasing 14-3-3 sigma expression with declining estrogen receptora and Efp expression defines malignant progression of endometrial carcinoma2005

    • Author(s)
      Nakayama H, Sano T et al.
    • Journal Title

      Pathology International 55

      Pages: 707-715

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Usefulness of liquid-based cytology specimens for the immunocytochemical study of p16 expression and Human papillomavirus testing : A comparative study using simultaneously sampled histology materials.2004

    • Author(s)
      Yoshida T, Sano T et al.
    • Journal Title

      Cancer (Cancer Cytopathology) 102

      Pages: 100-108

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunohistochemical expression of 14-3-3 sigma protein in various histological subtypes of uterine cervical cancers2004

    • Author(s)
      Sano T et al.
    • Journal Title

      Pathology International 54(10)

      Pages: 743-750

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] パピローマウイルス2004

    • Author(s)
      佐野孝昭 他
    • Journal Title

      病理と臨床(臨時増刊号)(病理診断における分子生物学) 22

      Pages: 292-297

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Usefulness of liquid-based cytology specimens for the immunocytochemical study of p16 expression and Human papillomavirus testing : A comparative study using simultaneously sampled histology materials2004

    • Author(s)
      Yoshida T, Sano T et al.
    • Journal Title

      Cancer (Cancer Cytopathology) 102

      Pages: 100-108

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunohistochemical expression of 14-3-3 sigma protein in various histological subtypes of uterine cervical cancers2004

    • Author(s)
      Sano T et al.
    • Journal Title

      Pathology International 54

      Pages: 743-750

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Papillomavirus (in japanese)2004

    • Author(s)
      Sano T et al.
    • Journal Title

      Pathology and Clinical medicine (supplemental) 22

      Pages: 292-297

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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