2005 Fiscal Year Final Research Report Summary
Personal endemic telomere length and analysis with an old disorder by FISH method
| Project/Area Number |
16590300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare |
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Principal Investigator |
NAKAMURA Ken-ichi Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare, Tokyo Metropolitan Institute of Gerontology, Research Scientist, 東京都老人総合研究所, 研究員 (60159069)
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| Co-Investigator(Kenkyū-buntansha) |
TAKUBO Kaiyo Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare, Tokyo Metropolitan Institute of Gerontology, Team Leader, 東京都老人総合研究所, 参事研究員 (00154956)
SHIMOMURA Naotaka Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare, Tokyo Metropolitan Institute of Gerontology, Research Assistant, 東京都老人総合研究所, 助手 (10158751)
ISHII Akiko Tokyo Metropolitan Foundation for Research on Aging and Promotion of Human Welfare, Tokyo Metropolitan Institute of Gerontology, Research Assistant, 東京都老人総合研究所, 助手 (60167244)
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| Project Period (FY) |
2004 – 2005
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| Keywords | telomere / Q-FISH / Tissue-FISH / successful aging / Q-FISH |
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| Research Abstract |
Using Southern blot analysis, we have measured telomere lengths in all human organs and tissues obtained at autopsy at the Tokyo Metropolitan Geriatric Medical Center, and accumulated data about changes of telomere shortening with aging. However, data obtained by Southern blot analysis is considered to have a number of inherent problems. We compared telomere lengths in the same cell lines and tissues measured by Southern blot analysis with those obtained by the Q-FISH method, and found they were correlated very strongly. Therefore, Southern blot analysis was confirmed to have high reliability for telomere length measurement. We then compared telomere lengths of four organs (brain, myocardium, liver and renal cortex) between two autopsy groups : one comprising individuals who were considered to be examples of "successful" aging and the other comprising individuals who were not. The results suggested that long telomeres in the four organs examined conferred some advantage for the achievement of successful aging. In addition, we confirmed that telomere lengths of these four organs showed strong correlations, suggesting that telomere length is a characteristic of each human individual. We then used the tissue FISH method to measure telomere lengths in normal esophageal mucosa, esophageal cancer, normal large bowel mucosa and large bowel cancer. Telomere lengths of both types of cancer cells were shorter than those of their normal counterpart epithelial cells and fibroblasts. In addition, we measured telomere lengths of 92 individual alleles from 46 metaphase chromosomes in cell lines by Q-FISH, and found that telomeres of specific chromosomes were not shorter than the median telomere lengths in all the cell lines. In cultured cell lines, X-chromosome loss, telomeric association with the X-chromosome, and tetraploidy (XXXX) were karyotypically evident at more than 50-60 PDL. Our results may be applicable to future detailed analysis of sex differences in life span.
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