2005 Fiscal Year Final Research Report Summary
Molecular function of Epstein-Barr virus nuclear antigens on the latent infection
| Project/Area Number |
16590395
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
HARADA Shizuko National Institute of Infectious Diseases, ウイルス第一部, 主任研究官 (10218646)
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| Project Period (FY) |
2004 – 2005
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| Keywords | EBV / virus / latent infection / nuclear protein / transactivation / EBNA |
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| Research Abstract |
Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that latently infects B-lymphocytes and causes their continuous proliferation into lymphoblastoid cell lines (LCL). EBV nuclear protein EBNA-2 and EBNA-LP are the first viral proteins expressed in lymphocytes. They cooperatively up-regulate EBV and cellular gene transcription, and are required for lymphocyte outgrowth into LCL. Previously, we discovered that mutant EBNALP that contains a 10-amino acid-truncation at its C-terminus does not have the ability to coactivate and it preferentially interacts with the EBNA2 acidic transactivating domain. Furthermore, the mutant EBNALP has a dominant negative effect on the coactivation activity of the wild-type EBNALP. The functional relevance of EBNALP in maintaining LCL cell growth is yet to be characterized. Therefore, we established cell clones derived from an LCL in which a dominant negative form of EBNALP (DNLP) is conditionally expressed by the Cre-loxP system. This was required in order to analyze the effect of DNLP expression on EB V induced cell proliferation. After drug addition, the generated LCL clones expressing DNLP showed retardation of cell proliferation and reduced cell viability. The results indicate that EBNALP plays a critical role in maintaining LCL growth and EB V induced cell proliferation.
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Research Products
(15 results)
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[Journal Article] Monoclonal antibodies against regions topologically surrounding the homodimeric beta-barrel interface of Epstein-Barr virus nuclear antigen-12005
Author(s)
Eda H, Ishii Y, Obayashi M, Harada S, Ito S, Fujita T, Ikeda M, Kusano S, Kitamura R, Suzuki C, Hara T, Watanabe M, Satoh H, Sugihara K, Yanagi K
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Journal Title
Virus Research 109
Pages: 87-94
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] An amino acid substitution attributable to insecticide-insensitivity of acetylcholinesterase inJapanese encephalitis vector mosquito, Culex tritaeniorynchus.2004
Author(s)
T.Nabeshima, A.Mori, T.Kozaki, Y.Iwata, O.Hidoh, S.Harada, S.Kasai, D.W.Severson, Y.Kono, T.Tomita.
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Journal Title
B.B.R.C. 313
Pages: 794-801
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Maintenance of serum immunoglobulin G antibodies to Epstein-Barr virus (EBV) nuclear antigen 2 in healthy indivisuals from different age groups in Japanese population with a high childhood incidence of asymptomatic primary EBV infection.2004
Author(s)
S.Harada, Y.Kamata, Y.Ishii, H.Eda, R.Kitamura, M.Obayashi, S.Ito, F.Ban, J.Kuranari, H.Makamura, T.Kuze, M.Hayashi, N.Okabe, H.Senpuku, N.Miyasaka, Y.Nakamura, H.Kanegane, K.Yanagi
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Journal Title
Clin.Diag.Lab.Immunol. 11(1)
Pages: 123-130
Description
「研究成果報告書概要(欧文)」より
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