2005 Fiscal Year Final Research Report Summary
Role of anti-adhesin, podocalyxin and adaptor proteins in glomerular injury
| Project/Area Number |
16590783
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Niigata University |
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Principal Investigator |
TAKEDA Tetsuro Niigata University, Medical and Dental Hospital, Assistant, 医歯学総合病院, 助手 (10361924)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Akihiko Niigata University, Graduate School of Medical and Dental Science, Associate Professor, 大学院・医歯学総合研究科, 客員助教授 (80293207)
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| Project Period (FY) |
2004 – 2005
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| Keywords | renal glomerulus / protein-protein interaction / phosphorylation / adaptor protein / podocyte |
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| Research Abstract |
We have previously demonstrated that anti-adhesin molecule, podocalyxin binds to the second PDZ domain of NHERF2 and ezrin in vitro and in vivo and that the interaction is important to maintain the unique organization of podocyte structure. In this research project, we investigated how the adaptor proteins interact with podocalyxin in glomerular injury.
1. We found that phosphrylation status of podocalyxin/NHERF2/ezrin comlex linked to the golmerular injury.
2. We identified the protein which interacted with first PDZ domain of NHERF2 by yeast two-hybrid system. Surprisingly, it was NHERF2 itself. This self-interaction was confirmed by GST pull-down assay and co-immunoprecipitation and was disrupted in severe injured glomeruli.
3. We analyzed the endocytic function of mouse immortalized glomerular epithlial cells that was established by Dr. Mundel like as in vivo millue.
4. We also found that the interaction between podocalyxin and adaptor proteins of above cell line was reduced in propotion to the angioteinsin II concentration.
5. When highly polarized MDCK cells were transfected to express podocalyxin, the adaptor proteins were re-localized to the apical submembranous domain and small G-protein, Rho A was activated. These results suggest podocalyxin may lead the adaptor proteins re-locate to the apical domain through the activation of Rho A.
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Research Products
(13 results)
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[Journal Article] Evidence for megalin-mediated proximal tubular uptake of L-FABP, a carrier of potentially nephrotoxic molecules2005
Author(s)
Oyama Y, Takeda T, Hama H, Tanuma A, Iino N, Sato K, Kaseda R, Ma M,..., Gejyo F, Saito A
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Journal Title
Laboratory Investigation 85(4)
Pages: 522-531
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Role of megalin, a proximal tubular endocytic receptor, in the pathogenesis of diabetic and metabolic syndrome-related nephropathies : protein metabolic overload hypothesis2005
Author(s)
Saito A, Takeda T, Hama H, Oyama Y, Hosaka K, Tanuma A, Kaseda R, Ueno M, Nishi S,..., Gejyo F
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Journal Title
Nephrology 10(S2)
Pages: S26-S31
Description
「研究成果報告書概要(和文)」より
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