2005 Fiscal Year Final Research Report Summary

Regulation of erythropoiesis mediated by heme

Research Project

Project/Area Number	16590920
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Hematology
Research Institution	TOHOKU UNIVERSITY
Principal Investigator	HARIGAE Hideo Tohoku University, Hospital, Associate Professor, 病院, 講師 (50302146)
Project Period (FY)	2004 – 2005
Keywords	heme / erythropoiesis / gene regulation
Research Abstract	To clarify the regulatory mechanism for erythropoiesis by heme, we performed differential expression analysis between wild-type and heme-deficient erythroblasts, which had been prepared from wild-type and δ-aminolevulinate synthase (ALAS2)-null mouse ES cells, respectively. From 8737 clones on cDNA array, clones which were highly expressed in wild-type erythroblasts were selected first and their expression was evaluated further for the erythroid-lineage specificity, heme-dependent expression, and expression in hematopoietic tissues in vivo. The final 4 genes were considered as the newly identified erythroid-specific heme-regulated genes. These 4 genes were uncoupling protein 2 (UCP2), nucleolar spindle-associated protein (NuSAP), cellular nucleic acid binding protein (CNBP), and a novel acetyltransferase-like protein. These findings suggest that heme may regulate a wide variety of hitherto unrecognized genes, and further analysis of these genes may clarify their role in erythroid cell differentiation.

(2 results)