2006 Fiscal Year Final Research Report Summary
Moderate hypothermia inhibits the death of differentiated PC12 cells mediated by simultaneous exposure to iron and ascorbic acid
| Project/Area Number |
16591082
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Osaka Medical College |
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Principal Investigator |
OGIHARA Toru Osaka Medical College, Faculty of Medicine, Assistant Professor, 医学部, 講師 (00211128)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Shuhei Osaka Medical College, Faculty of Medicine, Assistant Professor, 医学部, 講師 (10278525)
TAMAI Hiroshi Osaka Medical College, Faculty of Medicine, Professor, 医学部, 教授 (30179874)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Hypothermia / Free radical / Hypoxic ischemic encephalopathy / Iron / Ascorbic acid / PC 12 |
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| Research Abstract |
Hypothermia has attracted much attention as a leading approach for preventing brain damage due to neonatal hypoxic ischemic encephalopathy (HIE), but its appropriate clinical use remains unclear and the theoretical basis is yet to be established. We previously found that non-protein-bound iron and ascorbic acid (AA) levels were increased in the cerebrospinal fluid of HIE infants, and showed that FeSO4 and AA can synergistically induce apoptosis of PC12 cells. In this study, as an in vitro model of hypothermic therapy for HIE, we investigated the effect of hypothermia on Fe-AA cytotoxicity using differentiated PC12 cells. When the cells were exposed to Fe-AA for 48 h, moderate hypothermia (32℃ or 30℃) significantly improved viability (mean values;17.9 % of the unstressed control at 37℃ versus 56.4 % at 32℃ and 77.5 % at 30℃; p < 0.05, respectively). In contrast, mild hypothermia (35℃ or 34℃) had no beneficial effect and severe hypothermia (28℃) exacerbated cell death. A neuroprotective effect was only obvious if hypothermia (32℃) was started within 6 h of exposure. Even if hypothermia was started within 6 h of the insult, at least 24 h of hypothermia was necessary to improve cell viability. Caspase activity was significantly attenuated after 48 h of mild hypothermia. After 48 h of exposure to Fe-AA at 32℃, cell viability was preserved for at least the next 48 h. Taken together, our data were consistent with previous in vivo studies and suggest hypothermic inhibition of Fe-AA cytotoxicity may be one of the underlying mechanisms for cerebral hypothermic therapy.
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Research Products
(6 results)
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[Journal Article] Plasma KL-6 predicts the development and outcome of bronchopulmonary dysplasia2006
Author(s)
Tohru Ogihara, Kazuya Hirano, Takao Morinobu, Han-Suk Kim, Satoru Ogawa, Mayo Hiroi, Shinya Oue, Ryoichi Ban, Seigo Hira, Masashi Hasegawa, Shigeo Yamaoka, Masako Yasui
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Journal Title
Pediatric Research 60
Pages: 613-618
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Plasma KL-6 predicts the development and outcome of bronchopulmonary dysplasia2006
Author(s)
Tohru Ogihara, Kazuya Hirano, Takao Morinobu, Han-Suk Kim, Satoru Ogawa, Mayo Hiroi, Shinya Oue, Ryoichi Ban, Seigo Hira, Masashi Hasegawa, Shigeo Yamaoka, Masako Yasui
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Journal Title
Pediat Res. 60
Pages: 613-8
Description
「研究成果報告書概要(欧文)」より
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