2005 Fiscal Year Final Research Report Summary

The investigation of the mechanisms of upregulated collagen production of cytokine-induced skin fibrosis model.

Research Project

Project/Area Number	16591093
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Dermatology
Research Institution	KANAZAWA UNIVERSITY
Principal Investigator	TAKEHARA Kazuhiko Kanazawa University, School of Medicine, Professor, 医学系研究科, 教授 (50142253)
Co-Investigator(Kenkyū-buntansha)	FUJIMOTO Manabu Kanazawa University, School of Medicine, Associate Professor, 医学系研究科, 助教授 (90272591) SHIRASAKI Fumiaki Kanazawa University, School of Medicine, Lecture, 医学部附属病院, 講師 (10313644)
Project Period (FY)	2004 – 2005
Keywords	TGF-β / CTGF / fibrosis / c-myc / collagen
Research Abstract	We established cytokine induced skin fibrosis model. We injected TGF-β first which induces skin fibrosis and CTGF next which maintain skin fibrosis. In this study, we tried to clarify the detailed mechanism of this fibrosis model using in vitro study culture systems. (1) TGF-β alone induced collagen m-RNA. (2) CTGF alone had no effect. (3) TGF-β plus CTGF induced collagen m-RNA more than TGF-β alone. (4) TGF-β suppressed c-myc-mRNA in normal fibroblasts. (5) In scleroderma fibroblasts c-myc-mRNA was enhanced by TGF-β plus CTGF stimulation. Thus, abnormal response to scleroderma fibroblast may contribute to skin fibrosis in scleroderma.

(2 results)