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2005 Fiscal Year Final Research Report Summary

Expression of heat shock protein in the gastric mucosa of portal hypertensive rat

Research Project

Project/Area Number 16591330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionOita University

Principal Investigator

OHTA Masayuki  Oita University, Faculty of Medicine, Lecturer, 医学部, 講師 (80271104)

Co-Investigator(Kenkyū-buntansha) KITANO Seigo  Oita University, Faculty of Medicine, Professor, 医学部, 教授 (90169871)
SHIBATA Kohei  Oita University, Faculty of Medicine, Assistant Professor, 医学部, 助手 (50363560)
Project Period (FY) 2004 – 2005
Keywordsheat shock protein / HSP90 / HSP72 / eNOS / portal hypertensive gastropathy / adaptive cytoprotection
Research Abstract

Background Portal hypertensive (PHT) gastropathy is a clinical entity that is observed frequently in patients with liver cirrhosis. In PHT gastropathy, gastric mucosa is highly susceptible to mucosal injury caused by noxious agents. Heat shock protein (HSP) 72 and 90 has a protective effect against gastric mucosal injury. HSP 90 also leads activation of endothelial nitric oxide, (NO)-dependent vasodilation. However, it remains unclear how HSP influences the PHT gastric mucosa. The aim of the present study was to investigate HSP 72, HSP 90 and eNOS expression and the protective effect of HSP in the gastric mucosa of PHT rat.
Materials and Methods Male Sprague-Dawley rats weighing 300-375g were used for theses experiments. PHT rats were produced by staged portal vein occlusion. The gastric mucosal damage by 70% ethanol was evaluated by injury index (percent gross lesions), and adaptive cytoprotection using 10% ethanol was also evaluated. Expression of HSP 90, HSP 72 and eNOS mRNA in the g … More astric mucosa was quantified by real-time PCR.
Results Expression of mucosal HSP 90 mRNA was significantly decreased in the PHT rats compared with the sham-operated rats (P<0.05). Expression of mucosal eNOS mRNA was significantly higher in the PHT rats compared with the sham-operated rats (P<0.05). Gastric mucosal damage against 70% ethanol (10 ml/kg) was significantly increased in the PHT rats compared with the sham-operate rats (P<0.05). Gastric mucosal damage against 70% ethanol after administration of 10% ethanol was also significantly increased in the PHT rats compared with the sham-operated rats (P<0.05). Expression of mucosal HSP 90 and eNOS mRNA after administration of 10% ethanol was significantly increased in the PHT rats compared with the sham-operated rats (P<0.05). There were no significant differences in HSP72 mRNA after 10% ethanol administration between PHT and sham-operated rats.
Conclusion These results suggest that overexpression of HSP 90 may overproduce eNOS, and may impair the adaptive cytoprotection of PHT gastric mucosa to damage. Less

  • Research Products

    (12 results)

All 2006 2005 2004 Other

All Journal Article (12 results)

  • [Journal Article] 難治性食道静脈瘤に対する開腹下硬化療法(OIS)2006

    • Author(s)
      太田正之, 富永昌幸, 岩城堅太郎, 甲斐成一郎, 北野正剛
    • Journal Title

      消化器の臨床 (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prominent hepatic encephalopathy 19 years after splenopneumopexy in Budd-Chiari syndrome2006

    • Author(s)
      Tominaga M, Ohta M, Iwaki K, Uchida H, Endo Y, Kai S, Shibata K, Matsumoto T, Kitano S
    • Journal Title

      J Hepatobiliary Pancreat Surg (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of endothelin receptors in the gastric mucosa of portal hypertension.2006

    • Author(s)
      Kai S, Bandoh T, Ohta M, Matsumoto, Tominaga M, Kitano S.
    • Journal Title

      J Gastroenterol Hepato 21

      Pages: 242-250

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Open injection sclerotherapy for refractory esophageal varices (in Japanese)2006

    • Author(s)
      Ohta M, Tominaga M, Iwaki K, Kai S, Kitano S.
    • Journal Title

      Clinics in Gastoenterology (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Prominent hepatic encephalopathy 19 years after splenopneumopexy in Budd-Chiari syndrome.2006

    • Author(s)
      Tominaga M, Ohta M, Iwaki K, Uchida H, Endo Y, Kai S, Shibata K, Matsumoto T, Kitano S.
    • Journal Title

      J Hepatobiliary Pancreat Surg (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Analysis of risk factors for massive intraoperative bleeding during laparoscopic splenectomy2005

    • Author(s)
      Ohta M, Nishizaki T, Matsumoto T, Shimabukuro R, Sasaki A, Shibata K, Matsusaka T, Kitano S
    • Journal Title

      J Hepatobiliary Pancreat Surg 12

      Pages: 433-437

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 高齢者に特有な症状・症候別の救急処置 : 吐血・下血2005

    • Author(s)
      太田正之, 松本敏文, 石川浩一, 猪股雅史, 北野正剛
    • Journal Title

      臨床と研究 82(4)

      Pages: 65-68

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Analysis of risk factors for massive intraoperative bleeding during laparoscopic splenectomy.2005

    • Author(s)
      Ohta M, Nishizaki T, Matsumoto T, Shimabukuro R, Sasaki A, Shibata K, Matsusaka T, Kitano S.
    • Journal Title

      J Hepatobiliary Pancreat Surg 12

      Pages: 433-437

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Hematemesis and melena in the eldery (in Japanese).2005

    • Author(s)
      Ohta M, Matsumoto T, Ishikawa K, Inomata M, Kitano S.
    • Journal Title

      The Japanese Journal of Clinical and Experimental Medicine 82(4)

      Pages: 65-68

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 食道出血と内視鏡的治療2004

    • Author(s)
      太田正之, 甲斐成一郎, 富永昌幸, 松本敏文, 北野正剛
    • Journal Title

      救急医学 28(6)

      Pages: 665-670

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Esophageal variceal bleeding and endoscopic treatments (in Japanese).2004

    • Author(s)
      Ohta M, Kai S, Tominaga M, Matsumoto T, Kitano S.
    • Journal Title

      The Japanese Journal of Acute Medicine 28(5)

      Pages: 665-670

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of endothelin receptors in the gastric mucosa of portal hypertension

    • Author(s)
      Kai S, Bandoh T, Ohta M, Mastumoto T, Tominaga M, Kitano S
    • Journal Title

      J Gastroenterol Hepatol 21

      Pages: 242-250

    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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