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2006 Fiscal Year Final Research Report Summary

Study for epileptogenesis arising from the neuronal networks among amygdala, entorhinal cortex and hippocampus.

Research Project

Project/Area Number 16591462
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionToho University

Principal Investigator

NAGAO Takeki  Faculty of Medicine, Associate Professor, 医学部, 助教授 (20167555)

Co-Investigator(Kenkyū-buntansha) SEIKI Yoshikatu  Faculty of Medicine, Professor, 医学部, 助教授 (80147561)
KANO Toshiyuki  Faculty of Medicine, Assistant Professor, 医学部, 講師 (10297658)
AOKI Yoshinori  Faculty of Medicine, Research Associate, 医学部, 助手 (70349851)
Project Period (FY) 2004 – 2006
Keywordsslice / epilepsy / hippocampus / entorhinal cortex / amygdala / antiepileptic drug
Research Abstract

Recently, degeneration of entorhinal cortex, which fibers mostly connect to hippocampus proper, are playing an important role of epileptogenesis in limbic seizure. We have investigated this area with combined hippocampal slice which includes entorhinal cortices keeping neuronal connection. Thus far, the epileptogenesis of entorhinal cortices was elucidated by our former project.
In this project, we investigated the inhibitory effect of antiepileptic drugs on epileptic discharges recorded from the combined hippocampal slices with bath application of Quinolone and Flurbiprofen (antibacterial drug and analgesic respectively). The strongest inhibition was shown by Carbamazepine, followed by Phenobarbital and Valproic Acid.
We also developed combined parahippocampal slice including amygdala. Periodic oscillatory activity was identified in a quarter of these combined parahippocampal slices without any pharmacological manipulation. The neural networks were kept in these slices, identifying with de-synchronization of this activity after surgical separation of these slices to disconnect the parahippocampal neuronal networks.
Teses combined hippocampal and parahippocampal slices are useful tools for analyzing the underlying mechanisms of limbic seizure.

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Published: 2008-05-27  

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