2006 Fiscal Year Final Research Report Summary
Study on Control of Cell Death and Oxidative Stress in Ovary.
| Project/Area Number |
16591642
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUJIWARA Toshihiro The University of Tokyo, University of Tokyo Hospital, Assistant Professor (80219063)
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| Co-Investigator(Kenkyū-buntansha) |
YANO Tetsu 東京大学, 医学部附属病院, Associate Professor (90251264)
WADA Osamu 東京大学, 医学部附属病院, Assistant Professor (50372397)
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| Project Period (FY) |
2004 – 2006
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| Keywords | ovarian function / oxidative stress / anti-oxidative agent / coenzyme Q10 / vitamin C |
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| Research Abstract |
Ovarian aging is a major problem in infertility treatment. High aged women yield very low percentage of successful pregnancy even with assisted reproductive technology. One explanation is that oxidative stress over years may cause damage of oocyte. This leads to the idea that prevention of the damage can rescue oocyte quality, which improves successful pregnancy rate. To examine this hypothesis we performed basic experiment to test the efficacy of coenzyme Q10 (CoQ10) upon ovarian function in older female mice. CoQ10 (4ug/g wt) were orally administered for one month to C57BL/6J female mice of either three months, six months or ten months old. At the end of administration mice were sacrificed and both ovaries and vein blood were sampled. The following measurements were performed ; 1) CoQ10 levels in vein blood and ovarian tissue were measured using HPLC. 2) Both oxidative stress (d-ROMs) and anti-oxidative stress (BAP) were measured with FRAS4 (Wismerll, Japan) in 10-month old mice. 3)
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Weight of ovaries was measured. All data were compared with those of control mice to which only solvent of CoQ10 was administered.
1) In all age examined CoQ10 treated mice showed significantly higher levels of CoQ10 in both vein blood (about 1.5 times) and ovarian tissue (1.7-2 times). The CoQ10 ratio of vein blood/ovarian tissue did not differ in regard to age. 2) Mean level of d-ROMs was lower in CoQ10 treated mice (177 UCARR) compared to control (254 UCARR), while no significant difference was observed in the level of BAP. 3) Ovarian weight was about 30% heavier in CoQ10 treated mice than control.
This study revealed that orally administered CoQ10 was transported to ovarian tissue in all age examined. Increased intravenous CoQ10 levels reduced systemic oxidative stress. In addition ovarian weight showed bigger value by CoQ10 treatment. These results suggest that administration of CoQ10 may cause positive effect on ovary and thus it is expected that CoQ10 improves ovarian function in older animals. Less
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