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2005 Fiscal Year Final Research Report Summary

Determination of growth factors in head and neck carcinoma and development of treatment modality based on tumor characteristics.

Research Project

Project/Area Number 16591718
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionDepartment of Biology and Function in the Head and Neck Yokohama City University Graduate School of Medicine

Principal Investigator

TSUKUDA Mamoru  Yokohama City University, Graduate School of Medicine, Dept. of Biology and Function in the Head and Neck, Professor, 大学院・医学研究科, 教授 (70142370)

Co-Investigator(Kenkyū-buntansha) MIKAMI Yasukazu  Yokohama City University, Graduate School of Medicine, Dept. of Biology and Function in the Head and Neck, Associate Professor, 医学部, 準教授 (10322356)
HORIUCHI Choichi  Yokohama City University, Graduate School of Medicine, Dept. of Biology and Function in the Head and Neck, Assistant Professor, 医学部, 助手 (40295499)
Project Period (FY) 2004 – 2005
KeywordsHead and Neck Cancer / Growth Factor / Epidermal Growth Factor / Angiogenesis Factor
Research Abstract

The prognosis of advanced head and neck cancer is still poor. Conventional definitive treatments, i.e., surgical resection and/or radiotherapy have not improved the outcome because of locoregional relapses and distant metastases. It is indispensable to improve the outcome by examining biological characteristics of squamous cell carcinoma (SCC) cells which pathologically account for 90% of head and neck carcinomas in the head and neck. Based on this reason, we examined biological characteristics, e.g., growth and metastatic factors, of head and neck SCC cell lines established in our institute and anti-tumor effects of molecular targeted agents against these cell lines.
In these SCC cell lines, hetero- type mutation (2361 G to A) on exon 20 was found and IC_<50> of cell lines with the mutation in a sensitivity assay using gefitinib was significantly lower than that of cell lines without the mutation. This result indicates that the examination on gene mutation of EGFR on SCC cells might be useful to expect the effectiveness of gefitinib in patients with SCC of the head and neck.
Furthermore anti-tumor effects of bevacizumab, an inhibitor of VEGF pathway, and ZD6474 showing both inhibitory actions in VEGFR-2 and EGFR pathways were examined. A suppressive effect of bevacizumab in vitro was not observed, however bevacizumab showed an anti-tumor effect in vivo through induction of apoptosis and anti-angiogenesis. On the other hand, ZD6474 showed a suppressive effect on cell growth in vitro through inhibitions on phosphorylation of VEGFR-2,EGFR and MAPK. ZD6474 showed anti-tumor effects in vivo through induction of apoptosis and anti-angiogenesis. These results showed that ZD6474 had anti-tumor effects by two mechanisms, e.g., an inhibition on VEGFR-2 pathway resulting in anti-angiogenesis and an inhibition on EGFR pathway resulting in induction of apoptosis.

  • Research Products

    (32 results)

All 2006 2005

All Journal Article (30 results) Book (2 results)

  • [Journal Article] 高齢者の嚥下障害の実態とその治療-痴呆の影響と栄養サポートチーム(NST)介入の効果-2006

    • Author(s)
      平田佳代子, 佃 守
    • Journal Title

      耳鼻 52(補1)

      Pages: 25-39

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increased expression of matrix metalloproteinase-2 and 9 and human papilloma virus infection are associated with malignant transformation of sinonasal inverted papilloma.2006

    • Author(s)
      Katori H, Tsukuda M
    • Journal Title

      J Surg Oncol. 13;93(1)

      Pages: 80-85

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Histopathological parameters of recurrence and malignant transformation in sinonasal inverted papilloma.2006

    • Author(s)
      Katori H, Tsukuda M
    • Journal Title

      Acta Oto-Laryngologica 126

      Pages: 214-218

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Antitumor effects of IDN5109 on head and neck squamous cell carcinoma2006

    • Author(s)
      Sano D, Tsukuda M
    • Journal Title

      Oncol. Rep. 15(2)

      Pages: 329-334

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 最近の頭頸部癌化学療法の他施設共同研究2006

    • Author(s)
      佃 守
    • Journal Title

      耳鼻 51(補1)

      Pages: 15-21

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Increased expression of matrix metalloproteinase-2 and 9 and human papilloma virus infection are associated with malignant transformation of sinonasal inverted papilloma.2006

    • Author(s)
      Katori H, Tsukuda H
    • Journal Title

      J Surg Oncol. 13;93(1)

      Pages: 80-85

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Antitumor effects of IDN5109 on head and neck squamous cell carcinoma2006

    • Author(s)
      Sano D, Tsukuda M
    • Journal Title

      Oncol.Rep. 15(2)

      Pages: 329-334

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] 頭頸部扁平上皮癌におけるorgan preservationの試み2005

    • Author(s)
      田口亨秀, 佃 守
    • Journal Title

      癌と化学療法 32(13)

      Pages: 2030-2034

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Chemotherapy Study Group of Head and Neck Cancer : Randomized scheduling feasibility study of S-l for adjuvant chemotherapy in advanced head and neck cancer.2005

    • Author(s)
      Tsukuda M.
    • Journal Title

      Br. J. Cancer 93

      Pages: 884-889

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Evaluation of soluble adhesion molecules CD44 (CD44st, CD44v5, CD44v6), ICAM-1, and VCAM-1 as tumor markers in head and neck cancer.2005

    • Author(s)
      Kawano T, Tsukuda M
    • Journal Title

      Am. J. Otolaryngol. 26(5)

      Pages: 308-313

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 頭頸部癌の診断と治療2005

    • Author(s)
      佃 守
    • Journal Title

      耳鼻臨床 98(6)

      Pages: 746-747

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Reduced excision repair cross-complementing 1 expression associated with enhanced papilloma formation and fibroblast transformation after genrtic disruption of secreted protein acidic and rich in cysteine.2005

    • Author(s)
      Kato Y, Tsukuda M
    • Journal Title

      Int J Oncol 127(3)

      Pages: 759-768

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Markers of malignant transformation of sinonasal inverted papilloma.2005

    • Author(s)
      Katori H, Tsukuda M
    • Journal Title

      Eur. J. Surg. Oncol. 31(8)

      Pages: 905-911

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of SPARC in tongue carcinoma of stage II is associated with poor prognosis : An immunohistochemical study of 86 cases.2005

    • Author(s)
      Kato Y, Tsukuda M
    • Journal Title

      Int. J. Mol. Med. 16(2)

      Pages: 263-268

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Comparison of induction chemotherapy with docetaxel, cisplatin, and 5-fluorouacil (TPF) followed by radiation vs concurrent chemoradiotherapy with TPF in patients with locally advanced squamous cell carcinoma of the head and neck.2005

    • Author(s)
      Katori H, Tsukuda M
    • Journal Title

      Clin Oncol. 17(3)

      Pages: 148-152

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A pilot study of the translation, cultural adaptation and validation of the EORTC head and neck cancer quality of life questionnaire module (QLQ-H&N35) for use in Japan.2005

    • Author(s)
      Toth G, Tsukuda M
    • Journal Title

      Auris Nasus Larynx. 32(2)

      Pages: 175-183

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Four cases of simultaneous triple primary cancers of the hypopharynx, esophagus, and stomach.2005

    • Author(s)
      Ikeda Y, Tsukuda M
    • Journal Title

      Otolaryngol. Head Neck Surg. 132(5)

      Pages: 788-793

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Soluble CD44 standard, CD44 variant 5 and CD44 variant 6 and their relation to staging in head and neck cancer.2005

    • Author(s)
      Kawano T, Tsukuda M
    • Journal Title

      Acta Otolaryngol. 125(4)

      Pages: 392-397

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Acidic Extracellular pH Induces Matrix Metalloproteinase-9 Expression in Mouse Metastatic Melanoma Cells through the Phospholipase D-Mitogen-activated Protein Kinase Signaling.2005

    • Author(s)
      Kato Y, Tsukuda M
    • Journal Title

      J Biol Chem. 280(12)

      Pages: 10938-10944

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 頭頸部扁平上皮癌進行例に対するDocetaxel, Cisplatin and 5-FU(TPF)併用化学療法と放射線治療の検討2005

    • Author(s)
      香取秀明, 佃 守
    • Journal Title

      日耳鼻 108

      Pages: 157-163

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 科学放射線同時併用療法の晩期有害事象がQOLに与える影響2005

    • Author(s)
      小松正規, 佃 守
    • Journal Title

      耳鼻 51

      Pages: 35-41

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 頭頸部癌化学療法の進歩 頭頸部癌化学療法の過去・現在・未来2005

    • Author(s)
      佃 守
    • Journal Title

      JOHNS 21(1)

      Pages: 5-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Chemotherapy Study Group of Head and Neck Cancer : Randomized scheduling feasibility study of S-1 for adjuvant chemotherapy in advanced head and neck cancer.2005

    • Author(s)
      Tsukuda M
    • Journal Title

      Br.J.Cancer 93

      Pages: 884-889

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Evaluation of soluble adhesion molecules CD44 (CD44st, CD44v5,CD44v6), ICAM-1,and VCAM-1 as tumor markers in head and neck cancer.2005

    • Author(s)
      Kawano T, Tsukuda M
    • Journal Title

      Am.J.Otolaryngol. 26(5)

      Pages: 308-313

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Reduced excision repair cross-complementing 1 expression associated with enhanced papilloma formation and fibroblast transformation after gentric disruption of secreted protein acidic and rich in cysteine.2005

    • Author(s)
      Kato Y, Tsukuda M
    • Journal Title

      Int.J.Oncol. 127(3)

      Pages: 759-768

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Markers of malignant transformation of sinonasal inverted papilloma.2005

    • Author(s)
      Katori H, Tsukuda M
    • Journal Title

      Eur.J.Surg.Oncol. 31(8)

      Pages: 905-911

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of SPARC in tongue carcinoma of stage II is associated with poor prognosis : An immunohistochemical study of 86 cases.2005

    • Author(s)
      Kato Y, Tsukuda M
    • Journal Title

      Int.J. Mol.Med. 16(2)

      Pages: 263-268

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Comparison of induction chemotherapy with docetaxel, cisplatin, and 5-fluorouracil (TPF) followed by radiation vs concurrent chemoradiotherapy with TPF in patients with locally advanced squamaous cell carcinoma of the head and neck.2005

    • Author(s)
      Kawano T, Tsukuda M
    • Journal Title

      Clin Oncol. 17(3)

      Pages: 148-152

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A pilot study of the translation, cultural adaptation and validation of the EORTC head and neck cancer quality of life questionnaire module (QLQ-H & N35) for use in Japan.2005

    • Author(s)
      Toth G, Tsukuba M
    • Journal Title

      Auris Nasus Larynx. 32(2)

      Pages: 175-183

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Four cases of simultaneous triple primary cancers of the hypopharynx, esophagus, and stomach.2005

    • Author(s)
      Ikeda Y, Tsukuda M
    • Journal Title

      Otolaryngol.Head Neck Surg. 132(5)

      Pages: 788-793

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 化学療法の実際 頭頸部がん 臨床腫瘍内科学入門 (金倉護編)2005

    • Author(s)
      佃 守
    • Total Pages
      6
    • Publisher
      永井書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 頭頸部進行癌に対するChemoradiation (加我君孝、小室静雄編) 耳鼻咽喉科・頭頸部外科学の最新医療2005

    • Author(s)
      佃 守
    • Total Pages
      6
    • Publisher
      先端医療技術研究所
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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