2006 Fiscal Year Final Research Report Summary
Possibility of proteomic analyzation of saliva from patients with dry mouth
| Project/Area Number |
16592087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
KISHIMOTO Etsuo Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Associate Professor, 大学院医歯薬学総合研究科, 助教授 (20091316)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Shin Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, Associate Professor, 大学院医歯薬学総合研究科, 助教授 (40116471)
FUNABIKI Nobuo Okayama University Graduate School of Natural Science and Technology, Professor, 大学院自然科学研究科, 教授 (70263225)
NAKANISHI Toru Shujitsu University Pharmaceutical Sciences, Professor, 薬学部, 教授 (30243463)
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| Project Period (FY) |
2004 – 2006
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| Keywords | dry mouth / diagnosis / 2 dimension electrophoresis / eldery people / protein / saliva / medicine / proteomics |
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| Research Abstract |
The aim of this study was to describe the relationship between subjective feeling of dry mouth and medication intake, saliva flow, saliva composition in eldery people. Also the patient taking many medicine tend to suffer from dry mouth, then mechanisms and drugs of dry mouth induced with medicine were examined on the data base, Drugs in Japan. There were significant relationship between clinical diagnosis and mucous wet test and moisture test on tongue and between age and moisture test on tongue. Feeling of dry mouth, clinical diagnosis and results of diagnostic measurement were not coincided necessarily. When two dimensions electrophoresis were done, isoelectrophoresis of saliva was difficult because many proteins content were disappeared after centrifugation to remove residues of whole saliva contaminated with disrupted epithelial cells and oral microorganisms. The molecular weight of salivary protein varied abroad and especially high molecular weight of protein disturbed the analysis. As enzymatic degrad ation of saliva was tried, high molecular weight molecule were effectively degraded by trypsin, on the other hand degradation by Endo-α-NAc-Galactosamidase and a-N-Acetyl-Galactos-aminidase were limited. These results suggested that fragments of salivary proteins by enzymatic treatment should be applied onto 2-D electrophoresis. More study was need for development of clinical measurement for dry mouth.
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Research Products
(14 results)
