Studies of principles of the eukaryotic cell architecturebased on the nucleus-organelle consortium

2008 Fiscal Year Final Research Report

• Project/Area Number: 16GS0304
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: The University of Tokyo
• Principal Investigator: TANAKA Kan The University of Tokyo, 分子細胞生物学研究所, 客員教授 (60222113)
• Co-Investigator(Kenkyū-buntansha): 佐藤 直樹 東京大学, 大学院・総合文化研究科, 教授 (40154075) ; 野崎 久義 東京大学, 大学院・理学研究科, 准教授 (40250104) ; 河村 富士夫 立教大学, 理学部, 教授 (10126039)
• Project Period (FY): 2004 – 2008
• Keywords: Cyanidioschyzon merolae / organelle / chloroplast / mitochondria / genome / analysis / red algae / symbiosis / DNA polymerase

Research Abstract

真核細胞の基本的な作動原理を、その成立に深く関わったミトコンドリア・葉緑体の進化や機能に注目して研究した。動物・菌類を除く多くの真核細胞系統が一旦は葉緑体を持っていたとする'超植物界仮説'を提唱すると共に、共生由来オルガネラである葉緑体からのシグナルが、植物細胞周期の開始に必須であることを示した。さらに、細胞内に共存する3種ゲノムにおける遺伝情報の発現協調機構の解析などを通じ、原始的な真核細胞シゾンをモデル系とした細胞生物学の新分野を切拓いた。

Research Products

• [Journal Article] ChlH, the H subunit of Mg-chelatase, is ananti-sigma factor for SigE inSynechocystis sp (2009)
  • Author(s): Takashi Osanai, Masahiko Imashimizu, Asako Seki, Shusei Sato, Satoshi Tabata, Sousuke Imamura, Munehiko Asayama, Masahiko Ikeuchi and Kan Tanaka
  • Journal Title: PCC 6803. Proc. Natl.Acad. Sci. USA 106 Pages: 6860-6865
  • Peer Reviewed
• [Journal Article] Defensin-like polypeptide LUREs arepollen tube attractants secreted fromsynergid cells (2009)
  • Author(s): Okuda, S., Tsutsui, H., Shiina, K.,Sprunck, S., Takeuchi, H., Yui, R.,Kasahara, R. D., Hamamura, Y., Mizukami,A., Susaki, D., Kawano, N., Sakakibara,T., Namiki, S., Itoh, K., Otsuka, K.,Matsuzaki, M., Nozaki, H., Kuroiwa, T.,Nakano, A., Kanaoka, M. M., Dresselhaus,T., Sasaki, N. and Higashiyama, T.
  • Journal Title: Nature 458 Pages: 357-361
  • Peer Reviewed
• [Journal Article] Tetrapyrrolesignal as a cell cycle coordinator fromorganelle to nuclear DNA replication inplant cells (2009)
  • Author(s): Yuki Kobayashi, Yu Kanesaki, AyumiTanaka, Haruko Kuroiwa, TsuneyoshiKuroiwa and Kan Tanaka
  • Journal Title: Proc. Natl. Acad. Sci. USA 106 Pages: 803-807
  • Peer Reviewed
• [Journal Article] Gclust : trans-kingdom classification of proteinsusing automatic individual thresholdsetting (2009)
  • Author(s): Naoki Sato
  • Journal Title: Bioinformatics 25 Pages: 599-605
  • Peer Reviewed
• [Journal Article] Periodic gene expression during thehighly synchronized cell nucleus andorganelle division cycles in theunicellular red alga Cyanidioschyzonmerolae (2009)
  • Author(s): Takayuki Fujiwara, Osami Misumi, Kousuke Tashiro, Yamato Yoshida, KeijiNishida, Fumi Yagisawa, Sousuke Imamura,Masaki Yoshida, Toshiyuki Mori, Kan Tanaka,Haruko Kuroiwa and Tsuneyoshi Kuroiwa
  • Journal Title: DNA Res. 16 Pages: 59-72
  • Peer Reviewed
• [Journal Article] Plastidlocalization of the PEND protein ismediated by a noncanonical transit peptide (2009)
  • Author(s): K. Terasawa and N. Sato
  • Journal Title: FEBS J 276 Pages: 1709-1719
  • Peer Reviewed
• [Journal Article] Origins of a cyanobacterial6-phosphogluconate dehydrogenase inplastid-lacking eukaryotes (2008)
  • Author(s): Maruyama, S., Misawa, K., Iseki, M.,Watanabe, M. and Nozaki, H
  • Journal Title: BMC Evol.Biol 8 Pages: 151
  • Peer Reviewed
• [Journal Article] A crypticalgal group unveiled : a plastidbiosynthesis pathway in the oysterparasite Perkinsus marinus. (2008)
  • Author(s): Matsuzaki, M., Kuroiwa, H., Kuroiwa, T.,Kita, K. and Nozaki, H.
  • Journal Title: Mol. Biol.Evol 25 Pages: 1167-1179
  • Peer Reviewed
• [Journal Article] Purification andcharacterization of organellar DNApolymerases in the red algaCyanidioschyzon merolae (2008)
  • Author(s): T. Moriyama, K. Terasawa, M. Fujiwara and N. Sato
  • Journal Title: FEBS J 275 Pages: 2899-2918
  • Peer Reviewed
• [Journal Article] The plant-specificTFIIB-related protein, pBrp, is a generaltranscription factor for RNA polymerase I (2008)
  • Author(s): Sousuke Imamura, Mitsumasa Hanaoka and Kan Tanaka
  • Journal Title: EMBO J 27 Pages: 2317-2327
  • Peer Reviewed
• [Journal Article] Dynamics of RpaB-promoterinteraction during high light stress,revealed by chromatin immunoprecipitation(ChIP) analysis in Synechococcuselongatus PCC 7942 (2008)
  • Author(s): Mitsumasa Hanaoka and Kan Tanaka
  • Journal Title: Plant J 56 Pages: 327-335
  • Peer Reviewed
• [Journal Article] Polyethyleneglycol (PEG)-mediatedtransient gene expression in a red algaCyanidioschyzon merolae 10D (2008)
  • Author(s): Mio Ohnuma, Takashi Yokoyama, TakayukiInouye, Yasuhiko Sekine and Kan Tanaka
  • Journal Title: Plant CellPhysiol 49 Pages: 117-120
  • Peer Reviewed
• [Journal Article] Permuted tRNAgenes expressed via a circular RNAintermediate in Cyanidioschyzon merolae (2007)
  • Author(s): Soma, A., Onodera, A., Sugahara, J., Kanai, A., Yachie, N., Tomita, M, Kawamura,F. and Sekine, Y.
  • Journal Title: MolBiol. Evol 318 Pages: 1592-1595
  • Peer Reviewed
• [Journal Article] Phylogeny of primaryphotosynthetic eukaryotes as deduced fromslowly evolving nuclear genes (2007)
  • Author(s): Nozaki, H., Iseki, M., Hasegawa, M., Misawa, K., Nakada, T., Sasaki, N. and Watanabe, M.
  • Journal Title: Mol.Biol. Evol 24 Pages: 1592-1595
  • Peer Reviewed
• [Journal Article] Digalactosyldiacylglycerolis required for stabilization of theoxygen-evolving complex in photosystem II (2007)
  • Author(s): I. Sakurai, N. Mizusawa, H. Wada and N. Sato
  • Journal Title: Plant Physiol 145 Pages: 1361-1370
  • Peer Reviewed
• [Journal Article] A 100%-completesequence reveals unusually simple genomicfeatures in the hot spring red algaCyanidioschyzon merolae (2007)
  • Author(s): H. Nozaki, H. Takano, O. Misumi, K. Terasawa, M. Matuzaki, S. Maruyama, K. Nishida, F. Yagisawa, Y. Yoshida, T.Fujiwara, S. Takio, K. Tamura, S. J. Chung,S. Nakamura, H. Kuroiwa, K. Tanaka, N. Satoand and T. Kuroiwa
  • Journal Title: BMC Biol 5 Pages: 28
  • Peer Reviewed
• [Journal Article] Genomicand biochemical analysis of lipidbiosynthesis in the unicellularrhodophyte Cyanidioschyzon merolae : lackof plastidic desaturation pathway resultsin mixed pathway of galactolipid synthesis (2007)
  • Author(s): N. Sato and T. Moriyama
  • Journal Title: Eukaryotic Cell 6 Pages: 1006-1017
  • Peer Reviewed
• [Journal Article] DNA-binding and partial nucleoidlocalization of the chloroplast stromalenzyme ferredoxin : sulfite reductase (2007)
  • Author(s): K. Sekine, M. Fujiwara, M. Nakayama,T. Takao, T. Hase and N. Sato
  • Journal Title: FEBSJ 274 Pages: 2054-2069
  • Peer Reviewed
• [Journal Article] Mitochondrial genome of the moss Physcomitrella patens sheds new light on the mitochondrial evolution in land plants (2007)
  • Author(s): K. Terasawa, M. Odahara, Y. Kabeya, T. Kikugawa, Y. Sekine, M. Fujiwara and N.Sato
  • Journal Title: Mol.Biol.Evol 24 Pages: 699-709
  • Peer Reviewed
• [Journal Article] Induction of a group 2sigma factor, RpoD3, by high light and theunderlying mechanism in Synechococcuselongatus PCC 7942 (2007)
  • Author(s): Asako Seki, Mitsumasa Hanaoka, YukiAkimoto, Susumu Masuda, Hideo Iwasaki and Kan Tanaka
  • Journal Title: J.Biol.Chem 282 Pages: 36887-36894
  • Peer Reviewed
• [Journal Article] Isolated chloroplast machinery canactively constrict after stretching (2006)
  • Author(s): Yoshida, Y., Kuroiwa, H., Misumi, O.,Nishida, K., Yagisawa, F., Fujiwara, T, Nanamiya, H., Kawamura, F. and Kuroiwa, T
  • Journal Title: Science 313 Pages: 435-1438
  • Peer Reviewed
• [Journal Article] Males evolved from the dominant isogameticmating type (2006)
  • Author(s): Nozaki, H., Mori, T., Misumi, O., Matsunaga, S. and Kuroiwa, T
  • Journal Title: Curr. Biol 16 Pages: R1018-R1020
  • Peer Reviewed
• [Journal Article] Nirogen control of sugar catabolic geneexpression in Synechocystis sp. PCC 6803 (2006)
  • Author(s): Takashi Osanai, Sousuke Imamura,Munehiko Asayama, Makoto Shirai, IwaneSuzuki, Norio Murata and Kan Tanaka
  • Journal Title: DNA Res 13 Pages: 185-195
  • Peer Reviewed
• [Journal Article] Cytoplasmiclocalization of the single glutaminesynthetase in a unicellular red alga,Cyanidioschyzon merolae 10D (2006)
  • Author(s): Masaru Terashita, Shinichiro Maruyama and Kan Tanaka
  • Journal Title: Biosci.Biotechnol. Biochem 70 Pages: 2313-2315
  • Peer Reviewed
• [Journal Article] Characterization of MADS-boxgenes in charophycean green algae and itsimplication for the evolution of MADS-boxgenes (2005)
  • Author(s): Tanabe, Y., Hasebe, M., Sekimoto, H., Nishiyama, T., Kitani, M., Hensche, K., Mnster, T., Theiben, G., Nozaki, H., and Ito, M.
  • Journal Title: Proc. Natl. Acad. Sci. USA 102 Pages: 2436-2441
  • Peer Reviewed
• [Journal Article] Cyanidioschyzon merolaegenome : A tool for facilitating comparablestudies on organelle biogenesis inphotosynthetic eukaryotes (2005)
  • Author(s): Misumi, O., Matsuzaki, M., Nozaki, H., Miyagishima, S., Mori, T., Nishida, K.,Yagisawa, F., Yoshida, Y., Kuroiwa, H., and Kuroiwa, T.
  • Journal Title: Plant Physiol 137 Pages: 567-585
  • Peer Reviewed
• [Journal Article] Mass identification ofchloroplast proteins of endosymbiontorigin based on organism-optimizedhomologous protein groups (2005)
  • Author(s): Sato, N., Ishikawa, M., Fujiwara, M., and Sonoike, K.
  • Journal Title: GenomeInformatics 16 Pages: 56-68
  • Peer Reviewed
• [Journal Article] Identification of PamA as aPII-binding membrane protein important innitrogen-related and sugar-catabolic geneexpression in Synechocystis sp (2005)
  • Author(s): Osanai, T., Sato, S., Tabata, S., and Tanaka, K.
  • Journal Title: PCC 6803.J.Biol.Chem 281 Pages: 34684-34690
  • Peer Reviewed
• [Journal Article] Positive regulation of sugar catabolicpathways in the cyanobacteriumSynechocystis sp. PCC 6803 by the group 2sigma factor SigE (2005)
  • Author(s): Osanai, T., Nakano, T., Takahashi, H., Kanehisa, M., Asayama, M., Shirai, M., Suzuki, I., Murata, N., and Tanaka, K.
  • Journal Title: J.Biol.Chem 280 Pages: 30653-30659
  • Peer Reviewed
• [Journal Article] Microarray profiling of plastid geneexpression in a unicellular red alga,Cyanidioschyzon merolae (2005)
  • Author(s): Minoda, A., Hanaoka, M., Nagasawa, K., Horiuchi, M., Takahashi, H., and Tanaka,K.
  • Journal Title: Plant Mol. Biol 59 Pages: 375-385
  • Peer Reviewed
• [Journal Article] A Switchin RNA Polymerase Usage Mediated by tRNAGluDuring Chloroplast Development (2005)
  • Author(s): Hanaoka, M., Kanamaru, K., Fujiwara,M., Takahashi, H., and Tanaka, K.
  • Journal Title: EMBO Rep. 6 Pages: 545-550
  • Peer Reviewed
• [Journal Article] Zinc is a keyfactor in controlling alteration of twotypes of L31 protein in the Bacillussubtilis ribosome (2004)
  • Author(s): Nanamiya, H., Akanuma, G., Natori, Y., Murayama, R., Kosono, S., Kudo, T.,Kobayashi, K., Ogasawara, N., Park, S.-M.,Ochi, K., and Kawamura, F.
  • Journal Title: Mol. Microbiol 52 Pages: 273-283
  • Peer Reviewed
• [Journal Article] The minimal eukaryotic ribosomal DNA unitsin the primitive red alga Cyanidioschyzonmrolae (2004)
  • Author(s): Maruyama, S., Misumi, O., Ishii, Y., Asakawa, S., Shimizu, A., Sasaki, T., Matsuzaki, M., Shin-I, T., Nozaki, H.,Kohara, Y., Shimizu, N., and Kuroiwa, T.
  • Journal Title: DNA Res 11 Pages: 83-91
  • Peer Reviewed
• [Journal Article] Cyanobacterial genestransmitted to the nucleus beforedivergence of red algae in the Chromista (2004)
  • Author(s): Nozaki, H., Matsuzaki, M., Misumi, O.,Kuroiwa, H., Hasegawa, M., Higashiyama, T.,Shin-I, T., Kohara, Y., Ogasawara, N., and Kuroiwa, T.
  • Journal Title: J. Mol. Evol 59 Pages: 103-113
  • Peer Reviewed
• [Journal Article] Isolation of cycloheximide-resistantmutants of Cyanidioschyzon merolae (2004)
  • Author(s): Yagisawa, F., Nishida, K., Okano, Y., Minoda, A., Tanaka, K., and Kuroiwa, T.
  • Journal Title: Cytologia 69 Pages: 97-100
  • Peer Reviewed
• [Journal Article] Improvement ofculture conditions and evidence fornuclear transformation by homologousrecombination in a red alga,Cyanidioschyzon merolae 10D (2004)
  • Author(s): Minoda, A., Sakagami, R., Yagisawa, F.,Kuroiwa, T., and Tanaka, K.
  • Journal Title: Plant CellPhysiol 45 Pages: 667-671
  • Peer Reviewed
• [Presentation] "Super" Plant Kingdomproposed, rejected and reinstalled (2009)
  • Author(s): Nozaki, H.
  • Organizer: International Symposium on East AsianPlant Diversity and Conservation
  • Place of Presentation: Sapporo, Japan.
  • Year and Date: 2009-08-02
• [Presentation] 生物の基本原理を原子生物学的に解く為にシゾンはある (2009)
  • Author(s): 田中寛
  • Organizer: 第50回日本植物生理学会年会シンポジウム
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2009-03-25
• [Book] (2006)葉緑体核様体と分裂装置の可視化、新版植物の細胞を観る実験プロトコール(植物細胞工学シリーズ22) (2006)
  • Author(s): 寺澤公宏, 藤原誠,佐藤直樹
  • Total Pages: 149-155
  • Publisher: 秀潤社
• [Remarks] Nancy. R. Gough, Wait for Me. Sci. Signal2, ec27 (2009) (Science 誌の姉妹オンラインジャーナル、Editor' s Choice)
  • URL: http://stke.sciencemag.org/cgi/content/abstract/sigtrans:2/55/ec27
• [Remarks] (Nature Japan によるオンライン特集サイト記事)
  • URL: http://www.natureasia.com/japan/tokushu/detail.php?id=163
• [Remarks] 新聞報道
• [Remarks] 論文3に関して : 朝日新聞2009年1月6日夕刊、毎日新聞2009年1月10日、読売新聞2009年1月11日、東京新聞2009年1月27日科学面など多数
• [Remarks] 論文10に関して : 千葉日報2008年8月1日、読売新聞2008年8月2日。
• [Remarks] 論文14に関して : 東京新聞2007年6月26日、朝日新聞2007年10月22
• [Remarks] 論文16に関して : 毎日新聞2007年7月24日、大阪読売新聞2007年7月24日など多数。
• [Remarks] 論文22に関して : 読売新聞2006年12月19日、朝日新聞2007年1月9日、毎日新聞2007年1月17日など多数。
• [Remarks] 他、論文2、13、21についても新聞報道あり。