2018 Fiscal Year Final Research Report
On-chip Cell Measurement System with Top-down and Bottom-up Integration Approach
| Project/Area Number |
16H04301
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Research Collaborator |
Arai Fumihito
Honda Ayae
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | ナノマイクロメカトロニクス |
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| Outline of Final Research Achievements |
In this research project, as an on-chip cell measurement system integrating top-down and bottom-up approach, 1) Manipulation and cell injection of single fluorescence microsensor using multiple wavelength lights of 1064 nm and 808 nm as bottom-up cell measurement, 2) Long-term fluorescence measurement using hydrogel fluorescence microsensor by diffusion phenomenon of fluorescent dye in hydrogel as a top-down approach of cell measurement, were achieved.
We established the basic technologies for on-chip cell measurement using an optical measurement method, such as rapid and selective cell injection of fluorescence microsensor for intracellular measurement and extremely long measurement more than 1000 times using hydrogel fluorescence microsensor with fluorescence recovery in the sensor.
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| Free Research Field |
マイクロ・ナノロボティクス
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は，オンチップで操作可能な蛍光センサとチップ内に集積した蛍光センサアレイにより，細胞培養，環境制御，光ピンセットによる単一ウイルスの特定細胞への感染，細胞内局所計測，細胞集団計測に至るまでの一連の細胞解析プロセスをオンチップで行う点にある．一貫してオンチップで行うことで外乱の影響を受けにくくできる特徴がある．将来，ウイルス増殖を予防する新薬等の新たな診断・治療法に関する知見が得られる可能性があり，医療技術の発展にも貢献でき世界的に大きなインパクトとなり，生物科学との学際領域におけるブレイクスルーが期待される．
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