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2016 Fiscal Year Annual Research Report

ビーチロック形成機構に学ぶ新しい国土修復保全技術の開発

Research Project

Project/Area Number 16H04404
Research InstitutionHokkaido University

Principal Investigator

川崎 了  北海道大学, 工学研究院, 教授 (00304022)

Project Period (FY) 2016-04-01 – 2019-03-31
Keywordsビーチロック / 炭酸カルシウム / 微生物 / 国土保全
Outline of Annual Research Achievements

本研究の目的は,自然のビーチロック形成メカニズムを学ぶことで主に海岸域の微生物を利用した新しい国土修復保全に関する技術,すなわち,港湾岸壁などの海岸コンクリート構造物の修復保全および海浜堆積物の固化・安定化に関する基本技術を新たに開発することである。初年度である平成28年度に実施した内容は,(1)尿素分解菌に関する検討,(2)培養試験および強度発現メカニズムに関する検討,(3)小型円筒容器を用いた地盤の室内加速固化試験,の3項目であり,得られた主な成果の概要は,以下に示すとおりである。
(1)炭酸カルシウムの析出に適した尿素分解菌の候補を探索するため,国内外の海岸域および陸域の各地点から地盤材料を採取し,尿素分解菌の分離・単離を実施した。また,単離された多くの尿素分解菌を対象としたウレアーゼ活性試験を行い,ウレアーゼ活性が高いと判定された尿素分解菌の中から炭酸カルシウムの析出に適していると考えられる数種類の菌株を選抜した。
(2)選抜された菌株の遺伝子解析(16S rRNA遺伝子)を行い,炭酸カルシウムの析出に大きく関与している尿素分解菌のバイオセーフティレベルと能力について評価した。また,選抜された菌株を用いて異なる条件下における培養試験を行い,各菌株の最適な培養条件に関する基礎データを取得した。さらに,尿素分解菌による炭酸カルシウムの析出と地盤材料の強度発現に関するメカニズムについて考察した。
(3)最もウレアーゼ活性が高いと評価された尿素分解菌を用いて,地盤材料の室内シリンジ加速固化試験を実施した。室内加速固化試験では,効率的な炭酸カルシウムの析出による砂の固化条件について検討するため,培養液の菌数,固化溶液のカルシウムイオン濃度,試験時の温度,注入間隔などを変化させ,これらの試験条件が砂の加速固化に与える影響について針貫入試験により推定した一軸圧縮強さを用いて評価した。

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

初年度である平成28年度の研究の目的,実施計画,経費などの策定が適切であったこと,また,期待された研究成果がおおむね順調に得られたこと,さらに,研究代表者に健康上の問題発生しなかったことなどが,主な理由として考えられる。

Strategy for Future Research Activity

当初計画のとおり,平成29年度は平成28年度において実施した3つの研究項目の深化を図ると同時に,新たに(4)土槽を用いた地盤の室内加速固化試験,について研究を進める。

  • Research Products

    (31 results)

All 2017 2016 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 7 results,  Open Access: 7 results,  Acknowledgement Compliant: 1 results) Presentation (21 results) (of which Int'l Joint Research: 7 results,  Invited: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] アテネ大学(ギリシャ)

    • Country Name
      GREECE
    • Counterpart Institution
      アテネ大学
  • [Journal Article] Evaluation of porosity in biogrouted sand using microfocus X-ray CT2017

    • Author(s)
      S. Mitsuyama, K. Nakashima and S. Kawasaki
    • Journal Title

      International Journal of GEOMATE

      Volume: Vol. 12, Issue 31 Pages: 71-76

    • Peer Reviewed / Open Access
  • [Journal Article] Sand solidification through microbially induced carbonate precipitation for erosion control: Prospects in Bangladesh2016

    • Author(s)
      M. N. H. Khan, S. Kawasaki and M. R. Hassan
    • Journal Title

      Journal of Environmental Science and Natural Resources

      Volume: Vol. 9,No. 1 Pages: 59-61

    • DOI

      http://dx.doi.org/10.3329/jesnr.v9i1.30292

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Possibility for solidification of peaty soil by using microbes2016

    • Author(s)
      A. Sato, S. Kawasaki, T. Hata and T. Hayashi
    • Journal Title

      International Journal of GEOMATE

      Volume: Vol. 10, Issue 22 Pages: 2071-2076

    • Peer Reviewed / Open Access
  • [Journal Article] Soil reinforcement using calcium phosphate compounds2016

    • Author(s)
      S. Kawasaki and M. Akiyama
    • Journal Title

      ASEAN Engineering Journal

      Volume: Part C, Vol. 5, No. 1 Pages: 5-13

    • Peer Reviewed / Open Access
  • [Journal Article] Strengthening of sand cemented with calcium phosphate compounds using plant-derived urease2016

    • Author(s)
      R. A. N. Dilrukshi, J. Watanabe and S. Kawasaki
    • Journal Title

      International Journal of GEOMATE

      Volume: Vol. 11, Issue 25 Pages: 2461-2467

    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Coral sand solidification test through microbial calcium carbonate precipitation using Pararhodobacter sp.2016

    • Author(s)
      M. N. H. Khan, S. Shimazaki and S. Kawasaki
    • Journal Title

      International Journal of GEOMATE

      Volume: Vol. 11, Issue 26 Pages: 2665-2670

    • Peer Reviewed / Open Access
  • [Journal Article] Effect ofacidrainongeotechnicalpropertiesofresidualsoils2016

    • Author(s)
      Z. Bakhshipour, A. Asadi, B. B. K. Huat, A. Sridharan and S. Kawasaki
    • Journal Title

      Soils and Foundations

      Volume: Vol. 56, No. 6 Pages: 1008-1020

    • DOI

      http://dx.doi.org/10.1016/j.sandf.2016.11.006

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Development of novel coastal conservation technology learned from beachrock formation mechanism2017

    • Author(s)
      S. Kawasaki
    • Organizer
      1st International Workshop on Role of Microorganism and Thermo-Hydro-Chemico-Mechanical Process in Geoengineering and Geoscience
    • Place of Presentation
      The University of Ryukyus (Okinawa, Japan)
    • Year and Date
      2017-03-20 – 2017-03-20
    • Int'l Joint Research / Invited
  • [Presentation] 泥炭中の微生物数およびウレアーゼ活性度のばらつき2017

    • Author(s)
      佐藤厚子,林 憲裕,川﨑 了,畠 俊郎
    • Organizer
      土木学会北海道支部平成28年度年次技術研究発表会
    • Place of Presentation
      北見工業大学(北見市)
    • Year and Date
      2017-02-04 – 2017-02-05
  • [Presentation] Evaluation of porosity in biogrouted sand using microfocus X-ray CT2016

    • Author(s)
      S. Mitsuyama, K. Nakashima and S. Kawasaki
    • Organizer
      6th International Conference of Geotechnique, Construction Materials and Environment (GEOMATE 2016)
    • Place of Presentation
      Swissotel Le Concord Bangkok (Bangkok, Thailand)
    • Year and Date
      2016-11-14 – 2016-11-16
    • Int'l Joint Research
  • [Presentation] Effects of various powders on strength reinforcement of calcium phosphate compounds precipitated in sand specimens2016

    • Author(s)
      Dilrukshi, R.A.N. and Kawasaki, S.
    • Organizer
      5th International Doctoral Symposium on Sustainable Management of Resources and Environment in the 21st Century
    • Place of Presentation
      Hokkaido University (Sapporo, Japan)
    • Year and Date
      2016-11-10 – 2016-11-10
    • Int'l Joint Research
  • [Presentation] Whole-cell evaluation of bacterial urease activity for ground improvement techniques based on MICP2016

    • Author(s)
      M. Fujita, K. Nakashima and S. Kawasaki
    • Organizer
      4th Joint Seminar on Geoenvironmental Engineering and Recycling (GER 2016)
    • Place of Presentation
      Seoul National University (Seoul, Korea)
    • Year and Date
      2016-11-04 – 2016-11-05
  • [Presentation] Improvement of biogrout materials for conducting a field test2016

    • Author(s)
      F. Mizuno, K. Nakashima and S. Kawasaki
    • Organizer
      4th Joint Seminar on Geoenvironmental Engineering and Recycling (GER 2016)
    • Place of Presentation
      Seoul National University (Seoul, Korea)
    • Year and Date
      2016-11-04 – 2016-11-05
  • [Presentation] Expression and aggregation property of silica-polymerizing enzyme from sponge2016

    • Author(s)
      K. Nakashima, H. Oguri and S. Kawasaki
    • Organizer
      22nd Symposium of Young Asian Biological Engineers' Community (YABEC 2016)
    • Place of Presentation
      Miyazaki SEAGAIA Resort (Miyazaki, Japan)
    • Year and Date
      2016-10-27 – 2016-10-29
    • Invited
  • [Presentation] Anthropogenic Beachrock for Eco-Environment Shoreline Development2016

    • Author(s)
      L. R. Daryono, A. D. Titisari, I. W. Warmada and S. Kawasaki
    • Organizer
      2nd International Conference on Science and Technology 2016 (ICST 2016)
    • Place of Presentation
      Eastparc Hotel (Yogyakarta, Indonesia)
    • Year and Date
      2016-10-27 – 2016-10-28
    • Int'l Joint Research
  • [Presentation] Strength reinforcement of sand test specimens cemented with calcium phosphate compounds and different powders2016

    • Author(s)
      R.A.N. Nadeeka and S. Kawasaki
    • Organizer
      9th Asian Rock Mechanics Symposium
    • Place of Presentation
      The Stones Hotel - Legian Bali, Autograph Collection (Bali, Indonesia)
    • Year and Date
      2016-10-18 – 2016-10-20
  • [Presentation] 大腸菌により異種発現した海綿由来シリカ重合酵素の自己集合体形成と機能評価2016

    • Author(s)
      中島一紀, 小栗秀俊,川﨑 了
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場(富山市)
    • Year and Date
      2016-09-28 – 2016-09-30
  • [Presentation] Plant-derived urease induced sand cementation used in geotechnical engineering applications2016

    • Author(s)
      R. A. N. Dilrukshi and S. Kawasaki
    • Organizer
      International Conference on Geomechanics, Geo-energy and Geo-resources IC3G 2016
    • Place of Presentation
      Monash University (Melbourne, Australia)
    • Year and Date
      2016-09-28 – 2016-09-29
    • Int'l Joint Research
  • [Presentation] Comparison of Cementation Mechanism between Natural Beachrock and Anthropogenic Beachrock, Case Study: Okinawa Island, Japan2016

    • Author(s)
      L. R. Daryono, A. D. Titisari, I. W. Warmada and S. Kawasaki
    • Organizer
      41st Scientific Annual Meeting of Indonesian Association of Geophysicists
    • Place of Presentation
      The 7th Hotel Bandar Lampung (Bandar Lampung, Indonesia)
    • Year and Date
      2016-09-27 – 2016-09-29
  • [Presentation] Factors affecting the improvement of sand properties treated with microbially-induced calcite precipitation2016

    • Author(s)
      G. G. N. N. Amarakoon and S. Kawasaki
    • Organizer
      Geo-Chicago 2016: Sustainability, Energy, and the Geoenvironment
    • Place of Presentation
      Sheraton Grand Chicago (Chicago, USA)
    • Year and Date
      2016-08-14 – 2016-08-18
  • [Presentation] Sand cementation using the pH dependency of calcium phosphate compounds and the addition of various powders2016

    • Author(s)
      R. A. N. Dilrukshi and S. Kawasaki
    • Organizer
      Geo-Chicago 2016: Sustainability, Energy, and the Geoenvironment
    • Place of Presentation
      Sheraton Grand Chicago (Chicago, USA)
    • Year and Date
      2016-08-14 – 2016-08-18
  • [Presentation] Development of biogrouting using plant-derived urease and calcium phosphate compound2016

    • Author(s)
      M. Takatsu, R. A. N. Dilrukshi and S. Kawasaki
    • Organizer
      50th US Rock Mechanics / Geomechanics Symposium
    • Place of Presentation
      The Westin Galleria Houston (Houston, USA)
    • Year and Date
      2016-06-26 – 2016-06-29
  • [Presentation] シリカ重合酵素の作製と機能解析2016

    • Author(s)
      小栗秀俊,中島一紀,川﨑 了
    • Organizer
      資源・素材学会北海道支部平成28年度春季講演会
    • Place of Presentation
      室蘭工業大学(室蘭市)
    • Year and Date
      2016-06-18 – 2016-06-18
  • [Presentation] 固体バイオマスを用いた微生物燃料電池における基礎的検討2016

    • Author(s)
      吉村侑太,今成尭之,中島一紀,川﨑 了
    • Organizer
      資源・素材学会北海道支部平成28年度春季講演会
    • Place of Presentation
      室蘭工業大学(室蘭市)
    • Year and Date
      2016-06-18 – 2016-06-18
  • [Presentation] 金属親和性ペプチドを表層提示した微生物の開発2016

    • Author(s)
      岡本知之,中島一紀,川﨑 了
    • Organizer
      資源・素材学会北海道支部平成28年度春季講演会
    • Place of Presentation
      室蘭工業大学(室蘭市)
    • Year and Date
      2016-06-18 – 2016-06-18
  • [Presentation] 微生物を用いた地盤固化技術の試験施工を目指した尿素分解菌の探索2016

    • Author(s)
      高津桃子,新谷一之,中島一紀,川﨑 了
    • Organizer
      資源・素材学会北海道支部平成28年度春季講演会
    • Place of Presentation
      室蘭工業大学(室蘭市)
    • Year and Date
      2016-06-18 – 2016-06-18
  • [Presentation] Beachrocks cement characteristics and conditions of formation: Case study platanias beach, Chania, Greece2016

    • Author(s)
      A. Petropoulos, I. Baziotis, Ch. Anagnostou, N. Evelpidou and S. Kawasaki
    • Organizer
      14th International Conference of the Geological Society of Greece
    • Place of Presentation
      Aristotle University of Thessaloniki (Thessaloniki, Greece)
    • Year and Date
      2016-05-25 – 2016-05-27
    • Int'l Joint Research
  • [Presentation] Study of beachrocks in East Attica2016

    • Author(s)
      A. Karkani, N. Evelpidou, H. Maroukian and S. Kawasaki
    • Organizer
      14th International Conference of the Geological Society of Greece
    • Place of Presentation
      Aristotle University of Thessaloniki (Thessaloniki, Greece)
    • Year and Date
      2016-05-25 – 2016-05-25
    • Int'l Joint Research
  • [Remarks] 資源生物工学研究室ホームページ

    • URL

      http://labs.eng.hokudai.ac.jp/labo/bio-res/www/

  • [Patent(Industrial Property Rights)] 海岸・河岸保全工法2016

    • Inventor(s)
      川﨑 了,宇次原雅之
    • Industrial Property Rights Holder
      川﨑 了,宇次原雅之
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      特願2016-157306
    • Filing Date
      2016-08-10

URL: 

Published: 2018-01-16  

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