2018 Fiscal Year Final Research Report
Rapid analysis of glycoprotein glycans by capillary array affinity electrophoresis
| Project/Area Number |
16K08209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kindai University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 糖タンパク質糖鎖分析 / レクチン / エキソグリコシダーゼ / キャピラリー電気泳動 / 糖タンパク質性製剤 |
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| Outline of Final Research Achievements |
Oligosaccharides in glycoproteins play important roles in regulation of various biological functions. To monitor the quantitative glycosylation profiles, a highly sensitive and sophisticated method is required. We developed partial affinity capillary electrophoresis using lectins and exoglycosidases for the characterization of oligosaccharides derived from glycoproteins. A solution of a lectin or an exoglycosidase and a mixture of glycoprotein-derived oligosaccharides labeled with fluorescent tag were introduced to a neutrally coated capillary in this order, and then separated by application of a negative voltage. Application of various specificities of lectins indicated characteristic migration profiles of the oligosaccharides. Moreover, sequential injection of enzymes enabledsequencing of glycoprotein glycans.
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| Free Research Field |
薬品分析学
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| Academic Significance and Societal Importance of the Research Achievements |
現在、糖タンパク質性医薬品が数多く上市され、既にジェネリック製剤の投入時期を迎えた。過去に糖タンパク質製剤の糖鎖を十分に管理せず、重篤な事故を起こしたこともあり、人体に危険な非ヒト型糖鎖について、量が極めて微量であっても精度よく定量できる簡便な管理方法が求められている。本法はこのような目的にもっとも適していると考える。
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