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2018 Fiscal Year Final Research Report

Analysis of virus particle formation of paramyxovirus and development of new vaccines

Research Project

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Project/Area Number 16K08804
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Virology
Research InstitutionUniversity of Tsukuba

Principal Investigator

Takeuchi Kaoru  筑波大学, 医学医療系, 准教授 (00192162)

Research Collaborator KAMEYAMA Ken-ichiro  
MURAKAMI Kenji  
AIDA Yiko  
NAGATA Kyosuke  
Project Period (FY) 2016-04-01 – 2019-03-31
Keywordsパラミクソウイルス / Mタンパク質 / 牛下痢症 / 牛白血病 / ワクチン
Outline of Final Research Achievements

We successfully recovered membrane (M) protein gene-deficient bovine parainfluenza virus type 3 (BPIV3) using our reverse genetics system. We analyzed the function of the M protein in virus particle formation by transfecting M protein expressing plasmids into cells infected with M protein gene-deficient BPIV3. We found that a few amino acids in both amino and carboxy-termini of the M protein are important for BPIV3 virus particle formation. We generated recombinant BPIV3 expressing the E2 protein of bovine viral diarrhea virus (BVDV). When hamsters were infected with this recombinant BPIV3, neutralizing antibodies against BVDV were detected in sera of infected hamsters, indicating that this recombinant BPIV3 would be useful as a vaccine against BVDV.

Free Research Field

ウイルス

Academic Significance and Societal Importance of the Research Achievements

今回の結果は、牛パラインフルエンザウイルス3型(BPIV3)に非常に近縁なウイルスであるヒトパラインフルエンザウイルス3型(HPIV3)の粒子形成機構の解析に役立つと思われる。これらの結果は、まだワクチンの開発されていないHPIV3に対するワクチン開発あるいは抗ウイルス薬開発に役立つ可能性がある。また、我々が開発したBPIV3の遺伝子操作系は牛の各種ウイルス感染症に対するワクチン開発に利用出来る可能性がある。

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Published: 2020-03-30  

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