2018 Fiscal Year Final Research Report
Mitochondrial DNA deletions as a bioindicator of radiation exposure in papillary thyroid carcinoma
| Project/Area Number |
16K09805
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
中沢 由華 名古屋大学, 環境医学研究所, 助教 (00533902)
サエンコ ウラジミール 長崎大学, 原爆後障害医療研究所, 准教授 (30343346)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | mtDNA / Large-scale deletion / RNA-seq |
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| Outline of Final Research Achievements |
The aim of our study is to evaluate the mitochondrial DNA (mtDNA) deletion profile in radiation-induced and sporadic PTCs and in exposed to ionizing radiation (IR) human primary thyrocytes using next-generation sequencing as a potential individual molecular marker of radiation exposure. Using primary cultures of human thyrocytes exposed to IR (from 0.5 to 10 Gy), we found a significant dose-dependent increase of mtDNA deletions, while mtDNA content and common deletion (CD) content did not change. In radiation-induced PTC the number of large-scale deletions is higher than in sporadic.
In radiation-induced PTCs deleted mtDNA may persist for a long time after exposure, but only those whose size exceeds 1.9 kb are highly expressed. These findings suggest that RNA transcripts with large deletions involving CYTB, ND6, ND5, ND4, COX3, ATP6 and COX2 mtDNA genes may be a potential marker of radiation etiology of papillary thyroid carcinoma in exposed patients.
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| Free Research Field |
内分泌学、甲状腺学、分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
CYTB、ND6、ND5、ND4、COIII、ATP6及びCOⅡのmtDNA遺伝子領域における『大きな欠失（large-scale deletions）』を有するRNA転写産物が、放射線誘発PTC特異的な分子マーカーとなる可能性が示唆された。
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