2018 Fiscal Year Final Research Report
Comprehensive gene and protein analysis of ductal carcinoma in situ for novel prognostic biomarkers
| Project/Area Number |
16K10451
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Chiba University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
長嶋 健 千葉大学, 医学部附属病院, 准教授 (60292710)
宮崎 勝 国際医療福祉大学, 大学病院, 教授 (70166156)
榊原 雅裕 国際医療福祉大学, 医学部, 講師 (70375632)
羽山 晶子 千葉大学, 総合安全衛生管理機構, 助教 (80758251)
岩瀬 俊明 千葉大学, 大学院医学研究院, 特任助教 (90738254)
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| Research Collaborator |
Sato Mamoru
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 非浸潤性乳癌 / バイオマーカ― |
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| Outline of Final Research Achievements |
In this research project, we aimed to find biomarkers of high risk of invasion among the lesions diagnosed with noninvasive breast cancer by core needle biopsy. We tried to analyze protein expression, mRNA expression, and gene mutation using formalin fixed paraffin embedded breast cancer samples and samples which remained in washing saline after vacuum assisted biopsy (VAB). It was possible to detect PIK3CA mutations by high resolution melting analysis using the VAB sample. We also confirmed that gene expression analysis using RT-PCR with VAB sample is possible for four candidate genes that may be related to the invasive ability of non-invasive breast cancer.
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| Free Research Field |
乳腺外科
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、吸引式針生検の検体採取時に遺残する微量検体を使用して、遺伝子解析が可能な事を確認できた。本研究をさらに発展させることにより、非浸潤性乳がんに対する従来の形態学的病理診断を補完し、浸潤癌の存在を予測する為の悪性度マーカーの確立が可能になると期待される。
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