2018 Fiscal Year Final Research Report

Examination of differentiation induction new therapy using DFAT in human neuroblastoma

Research Project

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Project/Area Number	16K11355
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Pediatric surgery
Research Institution	Nihon University
Principal Investigator	KANEDA Hide 日本大学, 医学部, 助手 (30598967)
Co-Investigator(Kenkyū-buntansha)	古屋武史日本大学, 医学部, 研究医員 (20568539) 藤原恭子日本大学, 歯学部, 准教授 (40595708) 越永從道日本大学, 医学部, 教授 (70205376) 植草省太日本大学, 医学部, 助手 (70746338)
Project Period (FY)	2016-04-01 – 2019-03-31
Keywords	神経芽腫 / DFAT / 分化誘導 / BDNF
Outline of Final Research Achievements	Co-culture of neuroblastoma cells and DFAT induced differentiation of neuroblastoma cells. Treatment with retinoic acid and DFAT culture medium in combination resulted in induction of differentiation of neuroblastoma cells, rather than treatment with retinoic acid alone. Addition of BDNF neutralizing antibody in neuroblastoma cells cultured with DFAT resulted in further differentiation induction of neuroblastoma cells. When addition of BDNF neutralizing antibody in neuroblastoma cells cultured with DFAT culture medium, differentiation induction of neuroblastoma cells is suppressed. It was suggested that factors secreted by DFAT may induce differentiation of neuroblastoma cells by a signaling pathway via BDNF.
Free Research Field	小児外科学
Academic Significance and Societal Importance of the Research Achievements	本研究の学術的意義としては、DFATが分泌する液性因子は、神経芽腫細胞に対し直接的またはRAと協調的に作用し、分化誘導を促進することが新たに明らかになった。また、神経芽腫細胞の分化誘導に関わるDFATが分泌する液性因子の１つとしてBDNFの可能性が示唆された。 DFATは既存治療と併用することでさらなる治療効果の可能性を見出せたことについて、社会的意義がある。