2017 Fiscal Year Final Research Report
Fetastasis-supportive microenvironment induced by the interaction between metastatic tumor cells and stromal cells in the lymph node
| Project/Area Number |
16K15287
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Immunology
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KANDA Yasuhiro 新潟大学, 医歯学系, 助教 (00436768)
TAKEUCHI Arata 新潟大学, 医歯学系, 助教 (00360579)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | リンパ節転 / ストローマ細胞 / 組織微小環境 / 生体イメージング |
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| Outline of Final Research Achievements |
Cancer metastasis to the lymph node is crucial issue in disease pathophysiology, although the underlying mechanisms are largely unclear. This study aimed to reveal the cellular and molecular processes in lymph node metastasis through the detailed examinations focusing on the behavior of metastatic tumor cells within lymph node tissue structures and interactions between tumor cells and immune cells or stromal cells.
Subcutaneously injected mouse mammary tumor cells metastasized to draining lymph node until around 4 weeks and first settled in the subcapsular lymphatic sinus followed by expanding into tissue parenchyma. Inside the growing tumor, stromal network and blood vessels with unique features seemed to be formed and the infiltration of macrophages and T cells was also observed. In a coculture system, tumor cells selectively adhered to stromal cells, suggesting that metastatic tumor cells are likely to use stromal cells as scaffold for further invasion into the tissue in vivo.
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| Free Research Field |
免疫学
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