Identification of TDP-43 related genes using gene trap

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15481
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Hiroshima University
• Principal Investigator: Kawakami Hideshi 広島大学, 原爆放射線医科学研究所, 教授 (70253060)
• Co-Investigator(Kenkyū-buntansha): 森野 豊之 広島大学, 原爆放射線医科学研究所, 准教授 (10397953)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: TDP-43 / 筋萎縮性側索硬化症

Outline of Final Research Achievements

Aggregates of TAR DNA-binding protein of 43 kDa(TDP-43)are characteristic in motorneuron in amyotrophic lateral sclerosis. To identify genes influencing TDP-43 aggregate formation, we infected lentivirus from the library with CRISPR and the recognition sequence and destroy genes. We expressed TDP-43 with eGFP and isolated cells with enhanced signals by cell sorter. We sequenced DNA extracted from cells by next-generation sequencer, and identified 30 genes as TDP-43 aggregate formation related.

Free Research Field

神経内科学