Elucidation of oxygen-sensing molecular mechanisms on fetal pulmonary artery and ductus arteriosus

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15537
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Jikei University School of Medicine
• Principal Investigator: AKAIKE Toru 東京慈恵会医科大学, 医学部, 講師 (20647101)
• Co-Investigator(Kenkyū-buntansha): 南沢 享 東京慈恵会医科大学, 医学部, 教授 (40257332)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 肺動脈 / 動脈管

Outline of Final Research Achievements

In this study, we determined what factors dominantly expressed by an increase of oxygen concentration expressed in fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells. Fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells incubated under hypoxia for 48hours, and then incubated under normoxia for 24hours. The total RNA was extracted from these cells and was exhaustively gene-analyzed. DNA microarray revealed that transmembrane protein 104 and olfactory receptor 1262 were up-regulated by an increase of oxygen concentration in fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells. And ankyrin repeat domain 37 was down-regulated by an increase of oxygen concentration in fetal rat pulmonary arterial and ductus arteriosus smooth muscle cells. We will continue to investigate the molecular mechanism of these gene factors.

Free Research Field

小児循環器学