Analysis of N-glycans on vascular endothelial growth factor receptor-2 in diabetic retinopathy

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15728
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: Ishida Susumu 北海道大学, 医学研究院, 教授 (10245558)
• Co-Investigator(Kenkyū-buntansha): 西村 紳一郎 北海道大学, 先端生命科学研究院, 教授 (00183898) ; 野田 航介 北海道大学, 医学研究院, 准教授 (90296666)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 血管内皮増殖因子受容体-2 / N型糖鎖 / 糖尿病網膜症 / 低酸素

Outline of Final Research Achievements

Vascular endothelial growth factor (VEGF), a potent angiogenic and vascular permeability factor, plays a significant role in the pathogenesis of diabetic retinopathy. In the current study, we analyzed the structures of N-glycans on the receptor of VEGF, VEGFR-2. On the basis of preexisting database for glycan structure, the 16 structures of N-glycans on VEGFR-2 were identified in human retinal microvascular endothelial cells (HRMECs). However, no alteration of N-glycans was found on VEGFR-2 in HRMECs cultured under hypoxic condition, one of the representative pathological condition in diabetic retinopathy, for 24 hours compared to that under normoxic condition.

Free Research Field

眼科学