Analyses of endothelial cell dynamics in retinal angiogenesis

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15737
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Nagoya City University
• Principal Investigator: Uemura Akiyoshi 名古屋市立大学, 大学院医学研究科, 教授 (30373278)
• Co-Investigator(Kenkyū-buntansha): 西山 功一 熊本大学, 医学部附属病院, その他 (80398221)
• Co-Investigator(Renkei-kenkyūsha): MIURA Takashi 九州大学, 大学院医学研究院, 教授 (10324617) ; TERO Atsushi 九州大学, マス・フォア・インダストリ研究所, 准教授 (60431326) ; MIYATA Takaki 名古屋大学, 医学系研究科, 教授 (70311751)
• Research Collaborator: Kim Pilhan
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 網膜 / マウス / イメージング / 数理モデル / 内皮細胞 / ペリサイト / マクロファージ

Outline of Final Research Achievements

For the real-time monitoring of cell dynamics in live mouse retinas, we have developed an intravital imaging system utilizing confocal or two-photon laser scanning fluorescence microscopy. By exploiting this system, we successfully detected abnormalities in macrophage dynamics in pericyte-deficient mouse retinas following injections of an anti-PDGFRβ antibody, which reproduced characteristic features of human diabetic retinopathy. These achievements will contribute to elucidating dynamic movements of various types of retinal cells, including neurons, glial cells, and endothelial cells, under physiological and pathological conditions.

Free Research Field

眼科学