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2017 Fiscal Year Research-status Report

A realistic mechano-sensitive disease model in-vitro for cardiac tissue culture.

Research Project

Project/Area Number 16K18525
Research InstitutionKyoto University

Principal Investigator

Hoerning Marcel  京都大学, 高等研究院, 客員研究員 (20588496)

Project Period (FY) 2016-04-01 – 2019-03-31
KeywordsHydrogel / Mechanosensing / C2C12
Outline of Annual Research Achievements

We successfully published two works that are related to the current project. The first paper deals with the dynamics of the reversible dynamics of the hydrogel and its regulatory behavior to cells (Hoerning et al, Scientific Reports, 7, 7760, 2017). The supramolecular hydrogel is cross-linked by host-guest interactions between β-cyclodextrin (βCD) and adamantane, and were designed for the dynamic regulation of cell-substrate interactions. The magnitude of softening and stiffening of the substrate can be modulated by varying the concentrations of free, competing host molecules (βCD) in solutions. By changing the substrate elasticity at a desired time point, it is possible to switch the micromechanical environments of cells. Here, we use C2C12 muscle cells as a simple biological system to test the dynamics and response dynamics of the hydrogel to the cells. The second paper deals with cardiac dynamics. In particular we developed a novel method to extract and quantify the dynamics of line defects (Hoerning et al, Scientific Reports, 7, 7757, 2017). Line defects spatial separate electrophysiological oscillations in the tissue and are known to be a precursor of deceased states in heart, thus having potential to induce life-threatening fibrillation. In this study we show that line defects can translate, merge, collapse and form stable singularities with even and odd parity while maintaining a stable oscillation of the spiral wave in the tissue.

Current Status of Research Progress
Current Status of Research Progress

1: Research has progressed more than it was originally planned.

Reason

We have obtained new more complex dynamics of C2C12 cells on glass substrates to develop a more sophisticated method that can extract the membrane dynamics in 3D. A study that investigated the 3D dynamics of membrane proteins in currently under submission and was the first step to achieve the mentioned goal.

Strategy for Future Research Activity

Though our study progress is more smoothly than initially planned. We added more detailed steps and research foci in order to obtain a larger impact of our findings in future. So we stay on track with the initial research plan but added some sub-investigations that will enhance the scientific quality.

Causes of Carryover

Our results that we published so far are very promising and leading to even more important and interesting scientific questions. We believe that the money planed to used for the next fiscal year is well spend and necessary to proceed this study.

  • Research Products

    (5 results)

All 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Invited: 1 results)

  • [Journal Article] Dynamics of spatiotemporal line defects and chaos control in complex excitable systems2017

    • Author(s)
      Hoerning Marcel、Blanchard Francois、Isomura Akihiro、Yoshikawa Kenichi
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 7760

    • DOI

      10.1038/s41598-017-08011-z

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions2017

    • Author(s)
      Hoerning Marcel、Nakahata Masaki、Linke Philip、Yamamoto Akihisa、Veschgini Mariam、Kaufmann Stefan、Takashima Yoshinori、Harada Akira、Tanaka Motomu
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 7757

    • DOI

      10.1038/s41598-017-07934-x

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Spatiotemporal dynamics of line defects in cardiac tissue.2017

    • Author(s)
      Marcel Hoerning
    • Organizer
      3rd International Conference on Biomedical Technology
    • Invited
  • [Presentation] Interaction of cardiac tissue morphology and dynamics in mechano-sensitive environments during development.2017

    • Author(s)
      Marcel Hoerning
    • Organizer
      11. Workshop des Projekthauses NanoBioMater
  • [Presentation] Self-regulation of the mechanotransduction dynamics in developing cardiac tissues2017

    • Author(s)
      Marcel Hoerning, , Emilia Entcheva, Satoru Kidoaki, Kenichi Yoshikawa
    • Organizer
      EMBO/EMBL Symposium Mechanical Forces in Biology

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Published: 2018-12-17  

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