Development of new strategy for detection of disease denes

2009 Fiscal Year Final Research Report

• Project/Area Number: 17019063
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokai University
• Principal Investigator: INOKO Hidetoshi Tokai University, 医学部, 教授 (10101932)
• Co-Investigator(Kenkyū-buntansha): MANO Syuhei 名古屋市立大学, 大学院・システム自然科学研究科, 准教授 (20372948)
• Project Period (FY): 2005 – 2009
• Keywords: 相関解析 / マイクロサテライト / 多因子疾患 / 同祖性 / 連鎖解析 / 酵母ツーハイブリッド / ネットワーク / ゲノム創薬

Research Abstract

In order to identify susceptible gene for multi-factorial disease such as common disease, we have developed two methods, GWAS (genome-wide association study) using 30,000 microsatellites and detection of linkage between a trait and a marker in a random mating population without pedigree record. Utilizing these new methods, we could identify more than 30 disease susceptible genes. Towards genome-based drug discovery based on these disease genes, we have developed in silico network analysis and new yeast two hybrid system.

Research Products

• [Journal Article] Detecting linkage between a trait and a marker in a random mating population without pedigree record. (2009)
  • Author(s): Mano S, Endo TA, Oka A, Ozawa A, Gojobori T, Inoko H
  • Journal Title: PLoS ONE 4 Pages: e4956
• [Journal Article] A genome-wide association scan for asthma in a general Australian population. (2008)
  • Author(s): Hui J, Oka A, James A, Palmer LJ, Musk AW, Beilby J, Inoko H
  • Journal Title: Hum Genet 123 Pages: 297-306
• [Journal Article] Microsatellite markers for genome-wide association studies. (2007)
  • Author(s): Bahram S, Inoko H
  • Journal Title: Nature Reviews Genetics 8(2) Pages: 164
• [Journal Article] Single nucleotide polymorphism detection by polymerase chain reaction-restriction fragment length polymorphism. (2007)
  • Author(s): Ota M, Fukushima H, Kulski JK, Inoko H
  • Journal Title: Nature Protocol 2(11) Pages: 2857-6284
• [Journal Article] Whole genome association study of rheumatoid arthritis using 27, 039 microsatellites. (2005)
  • Author(s): Tamiya G, Shinya M, Imanish T, Ikuta T, Makino S, Okamoto K, Furugaki K, Matsumoto T, Mano S, Gojobori T, Bahram S, Inoko H
  • Journal Title: Hum Mol Genetics 14 Pages: 2305-2321
• [Journal Article] Genome-wide association studies define IL23R/IL12RB2 and IL10 as susceptibility loci for Behcet's disease.
  • Author(s): Mizuki N, Meguro A, Ota M, Ohno S, Shiota T, Kawagoe T, Okada T, Bahram S, 、Ishigatsubo Y, Inoko H
  • Journal Title: Nature Genetics (in press)
• [Presentation] Identification of NFKBIL1 as a susceptible locus of rheumatoid arthritis by genome- wide association using microsatellites and its functional analysis in steoclastogenesis. (2009)
  • Author(s): Inoko H
  • Organizer: Simposium in the10th Congress of International Ocular Inflammation Society
  • Year and Date: 2009-06-02
• [Presentation] Genome-wide scan of disease gene by association analysis using 30, 000 microsatellites. (2006)
  • Author(s): Inoko H
  • Organizer: The 8th International Meeting on Human Genome Variation on Complex Genome Analysis
  • Year and Date: 2006-09-15
• [Remarks] ホームページ:http://inoko. med. u-tokai. ac. jp 3万個のマイクロサテライトの位置、PCRプライマー、多型頻度などの情報に関するデータベース:
  • URL: http://www.jbirc.aist.go.jp/gdbs
• [Patent(Industrial Property Rights)] 尋常性乾癬検査用マーカー遺伝子 (2008)
  • Inventor(s): 猪子英俊、岡晃
  • Industrial Property Rights Holder: 東海大学、ジェノダイブファーマ
  • Industrial Property Number: 特許2008-000095
  • Filing Date: 2008-01-25