2006 Fiscal Year Final Research Report Summary
The study on changeable sequence length of an insertion mutation causing alopecia disease in the mouse hair keratin gene.
| Project/Area Number |
17300137
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | RIKEN |
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Principal Investigator |
YOSHIKI Atsushi RIKEN, Experimental Animal Division, Head, 実験動物開発室, 室長 (40212310)
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| Project Period (FY) |
2005 – 2006
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| Keywords | mouse / mutant / alopecia disease / dominant gene / insertion mutation / keratin gene / hair follicle / recessive gene |
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| Research Abstract |
Hague (Hag/Hag) is semidominat mutation by insertion sequence in keratin2-6g expressed specifically in the inner root sheath of the hair follicle. The mutant exhibits wavy coat at heterozygous state, and completely loses hairs at homozygous state. In the course of breeding homozygous Hag mouse, we have isolated recessive hag/hag mice with shortened insertion sequence. We investigated the change of length of the insertion sequence in the cross experiments of HagxHag, C3HxHag/Hag and C3HxHag/+ to clarify the mechanism of such generation of different allele during breeding. We used PCR primers to amplify the genomic fragments including the insertion sequence in the exon 1 of keratin2-6g. As results we could detect at least three different recessive alleles, h1(480 bp) > h2 > h (420 bp, recessive allele). We also detected in a few wavy mice with three different allele bands, H, h1 and h, suggesting the length change also occurred in somatic cells. The cross experiment of H/h x h1/h demonstrated that these parents generated progeny of H/H genotype, which proved the h or hi alleles can be extended to the longer H allele. We can conclude Hague mutation is an unique insertion whose length is changeable to shorter or longer form in the next generation
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Research Products
(24 results)
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[Journal Article] Lymphocytic choriomeningitis infection undetected by dirty-bedding sentinel monitoring and revealed after embryo transfer of an inbred strain derived from wild mice.2007
Author(s)
Ike F, Bourgade F, Ohsawa K, Sato H, Morikawa S, Saijo M, Kurane I, Takimoto K, Yamada Y K, Jaubert J, Berad M, Nakata H, Hirakawa N, Mekada K, Takakura A, Itoh T, Obata Y, Yosiki A, Montagutelli X
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Journal Title
Comparative Medicine 57
Pages: 272-281
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Fates of Cdh23/CDH23 with mutations affecting the cytoplasmic region2006
Author(s)
Yonezawa S, Yoshizaki N, Kageyama T, Takahasi T, Sano M, Tokita Y, Masaki S, Inaguma Y, Hanai A, Sakurai N, Yoshiki A, Kusakabe M, Moriyama A, Nakayama A
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Journal Title
Hum Mutat 27
Pages: 88-97
Description
「研究成果報告書概要(欧文)」より
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