2007 Fiscal Year Final Research Report Summary
Development of Diagnosis Method for Early Stage of Osteoarthritis using Fluorescence Correlation Spectroscopy
Project/Area Number |
17300166
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Biomedical engineering/Biological material science
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Research Institution | The Institute of Physical and Chemical Research |
Principal Investigator |
USHIDA Kiminori The Institute of Physical and Chemical Research, Advanced Science Institute, Eco-Soft Materials Research Unit, Research Unit Reader (60183018)
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Co-Investigator(Kenkyū-buntansha) |
SATO Masto Tokai University, Scollo of Medicint, Department of Orthopedic Surgery, Surgical Science, 准教授 (10056335)
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Project Period (FY) |
2005 – 2007
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Keywords | Florescence Correlation Spectroscopy / Anomalous Diffusion / Extracellular Matrix / Hyaluronan / Mucin / Laser / Osteoarthritis / Confocal Microscope |
Research Abstract |
To establish a diagnosis method for the early stage of Osteoarthritis (OA), we developed specially designed instruments of fluorescence correlation spectroscopy (FCS) using a confocal microscope. In this new method, we investigate the molecular transports in extracellular matrices (ECMs) which form inhomogeneous space with a mesh structure where anomalous diffusion occurs. In anomalous diffusion, different from normal diffusion, diffusion coefficient changes depending on the diffusion distance and the diffusion times. Therefore we developed a new method called "Sampling Volume Controlled (SVC) FCS" which enable us to record the distance dependence of diffusion coefficient (DDDC) in a direct manner. Within this research term we established the instrumentation and the theoretical treatment and anomalous diffusion comes to be recognized as an important process not only in ECM but also in intracellular spaces. We developed a method for diagnosis for an articular cartilage using its slice o
… More
f 20μm thickness put in a dye solution. The change in diffusion coefficients on protease treatment was observed showing that the destruction of the collagen mesh structure in the articular cartilage which mimics the symptom of OA and the principle of our new diagnosis method was confirmed. In the early term of this research we used the aqueous solution of hyaluronan as the model system of the synovial fluid. However, since the fluid contains another important component, mucin type proteoglycans, we searched an appropriate model system for basic research. As the result, we discovered a novel mucin in jellyfishes and named qniumucin. We performed the improvement of purification and the structural analysis to find that this new material is an exceptionally pure and homogenous mucin which is suitable to medical substances. We constructed a novel fulfillment for synovial fluid using qniumucin and hyaluronan and investigated using new FCS. We also found that this new fluid showed significant improvement in regeneration of damaged articular cartilage. Now we plan to extend our method for the development and improvement of this curing method. Less
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Research Products
(221 results)
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[Journal Article] Effects of growth factors on heparin-carrying polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering.2007
Author(s)
M. Sato, M. Ishihara, M. Ishihara, N. Kaneshiro, G. Mitani, T. Nagai, T. Kutsuna, T. Asazuma, M. Kikuchi, J. Mochida
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Journal Title
Journal of Biomedical Materials Research B, Applied Biomaterials 23
Pages: 181-188
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Compact soft X-ray source using Thomson scattering2006
Author(s)
Shigeru Kashiwagi, Ryunosuke Kuroda, Takashi Oshima, Fumio Nagasawa, Tomoaki Kobuki, Daisuke Ueyama, Yoshiyasu Hama. Masakazu Washio, Kiminori Ushida. Hitoshi Hayano, Junii Urakawa
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Journal Title
Jouranal of Applied Physics 98
Pages: 123302-01123302-06
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Tissue engineering of articular cartilage using an allograft of cultured chondrocytes in a membrane-sealed atelocollagen honeycomb-shaped scaffold (ACHMS scaffold).2005
Author(s)
K. Masuoka, T. Asazuma, M. Ishihara, M. Sato, H. Hattori, M. Ishihara, Y. Yoshihara, T. Matsui, B. Takase, M. Kikuchi, K. Nemoto
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Journal Title
Journal of Biomedical Materials Research B, Applied Biomaterials 75
Pages: 177-84
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Presentation] 早稲田大学における逆コンプトン散乱を用いた水の窓軟X線源の開発2008
Author(s)
五輪 智子, 五十嵐 千明, 樫野 多加志, 坂上 和之, 増田 明彦, 鷲尾 方一, 浦川 順治, 早野 仁司, 黒田 隆之助, 柏木 茂, 丑田 公規
Organizer
日本物理学会第63回年次大会
Place of Presentation
東大阪
Year and Date
20080300
Description
「研究成果報告書概要(和文)」より
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[Presentation] クラゲから抽出した新規ムチン2008
Author(s)
丑田 公規, 馬場崇行, 鵜澤 洵, 益田 晶子, 堂前 直, 浦井 誠, 谷口 佳代子, 山村 昌弘, 木平 孝治, 和田 啓男
Organizer
日本化学会第88春季年会
Place of Presentation
東京
Year and Date
20080300
Description
「研究成果報告書概要(和文)」より
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[Presentation] Development of a soft X-ray source based on inverse compton scattering at Waseda University2008
Author(s)
T. Gowa, C. Igaeashi, T. Kashino, K. Sakaue, A. Masuda, M. Washio, J. Urakawa, H. Hayano, R. Kuroda, S. Kashiwagi, K. Ushida
Organizer
63th Annual Meeting of Physical Society of Japan
Place of Presentation
Higashi Osaka
Year and Date
20080300
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A Novel Mucin Extracted From Jellyfish2008
Author(s)
K. Ushida, T. Baba, J. Uzawa, A. Masuda, N. Dohmae, M. Urai, K. Taniguchi, M. Yamamura, K. Kihira, H. Wada
Organizer
88th National Meeting of the Chemical Society of Japan
Place of Presentation
Tokyo
Year and Date
20080300
Description
「研究成果報告書概要(欧文)」より
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[Presentation] A Novel mucin extracted from jellyfish2008
Author(s)
K. Ushida. T. Baba, M. Urai, K. Taniguchi, J. Uzawa, K. Kihira, A. Masuda, N. Dohmae, M. Yamamura, H. Wada
Organizer
7th International Symposium on Biomimetic Materials Processing (BMMP-8)
Place of Presentation
名古屋
Year and Date
20080100
Description
「研究成果報告書概要(和文)」より
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[Presentation] A Novel mucin extracted from jellyfish2008
Author(s)
K. Ushida. T. Baba, M. Urai, K. Taniguchi, J. Uzawa, K. Kihira, A. Masuda, N. Dohmae, M. Yamamura, H. Wada
Organizer
7th International Symposium on Biomimetic Materials Processing (BMMP-8)
Place of Presentation
Nagoya
Year and Date
20080100
Description
「研究成果報告書概要(欧文)」より
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[Presentation] クラゲ体内から発見した新規ムチン2007
Author(s)
丑田 公規, 馬場 崇行, 浦井 誠, 谷口 佳代子, 鵜澤 洵, 木平 孝治
Organizer
第18回日本MRS学術シンポジウム
Place of Presentation
東京
Year and Date
20071200
Description
「研究成果報告書概要(和文)」より
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[Presentation] Recent progress of picosecond RF-Gun system at Waseda University2007
Author(s)
M. Washio, K. Sakaue, K. Komiya, T. Gowa, A. Masuda, A. Murata, Y. Kato, Y. Kamiya, S. Kashiwagi, R. Kuroda, H. Hayano, J. Urakawa, X. J. Wang, K, Ushida
Organizer
7th Interanational Symposium on Advanced Scienee Research (ASR2007)
Place of Presentation
東海村
Year and Date
20071100
Description
「研究成果報告書概要(和文)」より
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[Presentation] Recent progress of picosecond RF-Gun system at Waseda University2007
Author(s)
M. Washio, K. Sakaue, K. Komiya, T. Gowa, A. Masuda, A. Murata, Y. Kato, Y. Kamiya, S. Kashiwagi, R. Kuroda, H. Hayano, J. Urakawa, X. J. Wang, K. Ushida
Organizer
7th International Symposium on Advanced Science Research (ASR2007)
Place of Presentation
Tokai-mura
Year and Date
20071100
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 軟X線顕微鏡の開発に向けた化学増幅型フォトレジストの評価2007
Author(s)
五輪 智子, 五十嵐 千明, 丑田 公規, 大島 明博, 樫野 多加志, 柏木 茂, 黒田 隆之助, 坂上 和之, 濱 義昌, 増田 明彦, 鷲尾 方一
Organizer
第50回放射線化学討論会
Place of Presentation
宇治
Year and Date
20071000
Description
「研究成果報告書概要(和文)」より
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[Presentation] 早稲田大学における逆コンプトン散乱を用いた水の窓領域軟X線源の開発2007
Author(s)
増田 明彦, 五十嵐 千明, 丑田 公規, 浦川 順治, 樫野 多加志, 柏木 茂, 神谷 好郎, 黒田 隆之助, 五輪 智子, 坂上 和之, 早野 仁司, 森山 亮, 鷲尾方
Organizer
日本物理学会第62回年次大会
Place of Presentation
札幌
Year and Date
20070900
Description
「研究成果報告書概要(和文)」より
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[Presentation] Development of X-ray generater for "Water Window region using inverted Compton scattering as Waseda University"2007
Author(s)
A. Masuda, C. Igarashi, K. Ushida. J. Urakawa, T. Kashino, S. Kashiwagi, Y. Kamiya, R. Kuroda, T. Gowa, K. Sakaue, H. Hayano, R. Moriyama
Organizer
62th Annual Meeting of Physical Society of Japan
Place of Presentation
Sapporo
Year and Date
20070900
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 早稲田大学におけるパルスラジオリシスシステムの現伏2007
Author(s)
小宮 圭太, 坂上 和之, 永井 宏幸, 野本 知章, 濱 義昌, 藤田 晃宏, 鷲尾 方一, 丑田 公規, 柏木 茂, 神谷 好郎, 黒田 隆之助
Organizer
第4回日本加速器学会年会・第32回リニアック技術研究会
Place of Presentation
和光
Year and Date
20070800
Description
「研究成果報告書概要(和文)」より
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[Presentation] Improvement of soft X-ray generation system based on laser Compton Scattering2007
Author(s)
T. Gowa, Y. Kamiya, A. Masuda, R. Moriyama, K. Sakaue, M. Washio, R. Kuroda, S. Kashiwagi, H. Hayano, J. Urakawa, K. Ushida
Organizer
2007 Particle Accelerator Conference (PAC07)
Place of Presentation
Albuquerque, USA
Year and Date
20070600
Description
「研究成果報告書概要(和文)」より
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[Presentation] Improvements of time resolution in compact pulse radiolysis system2006
Author(s)
H. Nagai, M. Kawaguchi, K. Komiya, Y. Kamiya, K. Sakaue, Y. Hama, M. Washio, T. Nomoto, K. Ushida, S. Kashiwagi, R. Kuroda
Organizer
7th International Symposium on Ionizing Radiati on and Polymers (IRaP2006)
Place of Presentation
Antalya, Turkey
Year and Date
20060900
Description
「研究成果報告書概要(和文)」より
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[Presentation] Improvements of time resolution in compact pulse radiolysis system2006
Author(s)
H. Nagai, M. Kawaguchi, K. Komiya, Y. Kamiya, K. Sakaue, Y, Hama, M. Washio, T. Nomoto, K. Ushida. S. Kashiwagi, R. Kuroda
Organizer
7th International Symposium on Ionizing Radiation and Polymers (IRaP 2006)
Place of Presentation
Antalya, Turkey
Year and Date
20060900
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 早稲田大学におけるピコ秒パルスラジオリシスシステムの構築と現状2006
Author(s)
永井 宏幸, 川口 昌朗, 小宮 圭太, 野木 知章, 坂上 和之, 神谷 好郎, 丑田 公規, 柏木 茂, 黒田 隆之助, 濱 義昌, 鷲尾 方一
Organizer
第31回リニアック技術研究会
Place of Presentation
仙台
Year and Date
20060800
Description
「研究成果報告書概要(和文)」より
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[Presentation] 早稲田大学における逆コンプトン散乱を用いた水の窓領域軟X線生成2006
Author(s)
増田 明彦, 丑田 公規, 浦川 順治, 柏木 茂, 神谷 好郎, 黒田 隆之助, 五輪 智子, 坂上 和之, 早野 仁司, 森山 亮, 鷲尾 方一
Organizer
日本物理学会2007年春季大会
Place of Presentation
八王子
Year and Date
20060800
Description
「研究成果報告書概要(和文)」より
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[Presentation] 逆コンプトン散乱を用いた軟X線生成とその応用2005
Author(s)
南口 修一, 樋爪 健太郎, 斉藤 卓, 上山 大輔, 黒田 隆之助, 大島 明博, 鷲尾 方一, 浦川 順治, 早野 仁司, 柏木 茂, 丑田 公規
Organizer
第54回高分子学会年次大会
Place of Presentation
横浜
Year and Date
20050500
Description
「研究成果報告書概要(和文)」より
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[Presentation] Soft X-Ray Generation Using Inverse Compton Scattering and Its Application2005
Author(s)
S. Kawaguchi, K. Hizume, T. Saito, D. Ueyama, R. Kuroda, A. Ohshima, M. Washio, J. Urawa, H. Hayano, S. Kashiwagi, K. Ushida
Organizer
54th Annual Meeting of the Society of Polymer Science
Place of Presentation
Japan, Yokohama
Year and Date
20050500
Description
「研究成果報告書概要(欧文)」より
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[Patent(Industrial Property Rights)] 新規ムチン型糖タンパク質2007
Inventor(s)
益田 晶子, 丑田 公規, 堂前 直, 宮脇 敦史, 古川 英光
Industrial Property Rights Holder
独立行政法人理化学研究所
Industrial Property Number
特願2005-234108PCT WO2007/020889(2007/2/22)
Filing Date
2007-02-22
Description
「研究成果報告書概要(和文)」より
Overseas
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