2007 Fiscal Year Final Research Report Summary
Construction of artificial symbiotic community using Chlorella-symbiont association and it's application
| Project/Area Number |
17310042
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | University of Tsukuba |
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Principal Investigator |
TANAKA Hideo University of Tsukuba, Professor Emeritus (40015657)
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| Co-Investigator(Kenkyū-buntansha) |
AOYAGI Hideki University of Tsukuba, Graduate School of Life and Environmental Sciences, Associate Professor (00251025)
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| Project Period (FY) |
2005 – 2007
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| Keywords | symbiosis / Chlorella / chemical communication / physical communication / sheath / Extracellular released organic carbon / mixed culture / waste water treatment |
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| Research Abstract |
Chlorella sorokiniana IAM C-212 has long been maintained in slant culture as a mixed strain, representing an associated natural microbial consortium. The consortium was separated and four nonalgal constituents, a fungal strain (Acremonium sp.), and three bacterial strains (Ralstonia pickettii, Sphingomonas sp. DD38, Microbacterium trichotecenolyticum) were isolated and identified. The interaction between Chlorella and its symbionts under photoautotrophic conditions involved both mutualism and commensalisms. Sphingomonas sp. And M. trichotecenolyticum were harbored on the sheath produced by Chlorella, while Acremonium sp. And R. pickettii directly adhered to the Chlorella cell surface by using lectin-like substances. The sheath is composed of sugars, amino acids, vitamins and metals. A novel artificial medium that imitates photoautotroph-heterotroph interaction was developed. With the artificial medium, it was possible to selectively isolate some novel bacterial strains. The sheath is a suitable habitat for several symbiotic microorganisms because it ensures close proximity between the C. sorokiniana and symbionts. The sheath was increased by addition of calcium chloride. The sheath resulted in coflocculation of C. sorokiniana and the associated symbiotic bacteria, thus strengthening the bacterial-Chlorella symbiotic association. An application of this technique was demonstrated by constructing a complex of C. sorokiniana and a propionate-degrading bacterium (PDS1). Although propionate inhibited the growth of axenic C. sorokiniana, the C. sorokiniana-PDS1 complex (CPC) showed good growth in a medium containing a high concentration of propionate. The CPC was also used for simultaneous removal of organic acids, nitrate, ammonia and phosphate.
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Research Products
(13 results)
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[Journal Article] Development of a novel artificial medium based on utilization of algal photosynthetic metabolites by symbiotic heterotrophs.2008
Author(s)
Watanabe, K., Imase, M.,Aoyagi, H., Ohmura, N., Saiki, H., Tanaka, H.
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Journal Title
Journal of Applied Microbiology
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Composition of the sheath produced by the green alga Chlorella sorokiniana2006
Author(s)
Watanabe, K., Imase, M., Sasaki, K., Ohmura, N., Saiki, H., Tanaka, H.
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Journal Title
Letters in Applied Microbiology 42
Pages: 538-543
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Symbiotic association in Chlorella culture and application of its mechanism to the microbial industry2005
Author(s)
Watanabe, K., Imase, M., Aoyagi, H., Ohmura, N., Saiki, H., Tanaka, H.
Organizer
Annual Meeting 2005, The Society for Biotechnology, Japan
Place of Presentation
Tsukuba
Year and Date
2005-11-17
Description
「研究成果報告書概要(欧文)」より
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[Presentation] The screening of microorganisms that produce adhesion substances by using Chlorella EOC medium for composing artificial symbiotic association2005
Author(s)
Murata, M., Imase, M., Watanabe, K., Aoyagi, H., Tanaka, H.
Organizer
Annual Meeting 2005, The Society for Biotechnology, Japan
Place of Presentation
Tsukuba
Year and Date
2005-11-17
Description
「研究成果報告書概要(欧文)」より
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