2007 Fiscal Year Final Research Report Summary
Toward Practical Usz of Microchip Immunoassay Systems
| Project/Area Number |
17310087
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Microdevices/Nanodevices
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| Research Institution | Nagoya University (2006-2007)
Kanagawa Academy of Science and Technology (2005) |
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Principal Investigator |
MANABU Tokeshi Nagoya University, Graduate School of Engineering, Associate Professor (60311437)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMORI Takehiko The University of Tokyo, Graduate School of Engineering, Professor (60214821)
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| Project Period (FY) |
2005 – 2007
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| Keywords | Microchip / Immunoassay / Photopolymer / Fluorescence Polarization |
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| Research Abstract |
We have developed three types of new immunoassay formats.
A microchip-based semi-automated heterogeneous immunoassay was developed wherein a flow injection-like system and repeatable-use gel were employed. A regeneration buffer for antigen-antibody interaction of brain natriuretic peptide (BNP) was found which did not lose binding activity of the antibody on the gels and integration of such an immunoassay scheme into a semi-automated microfluidics system would facilitate repeated-use heterogeneous immunoassay. The concentration of BNP in the range of 0.1-100 pg/ml was successfully determined using the system and protocol developed here.
We developed a new platform for chip-based immunoassay to create the effective reaction sites of immunological reaction between antigen and antibody. The reaction sites were fabricated by the UV photopolymerization of a solution of photopolymer and the antibody-immobilized polystyrene beads. Our platform has many advantages ; (i) rapid analysis (a few min
… More
utes), (ii) easy handling (no need pump and valve), (iii) low-cost (disposable), (iv) small volume(I droplets(0.25gL)of sample and reagents), and(v)high sensitivity(2 orders higher than conventional method). In order to evaluate our platform, we carried out(sandwich)immunoassay for a-fetoprotein(AFP), which is known as a tumor marker. In the case of 1-min incubation(total assay time : ca. 3 min), the limit of detection (LOD) was 10 ng/ml. This value is compared to that of conventional method using a microtiter plate with 4 h of total assay time. The LOD for &min incubation was 2 orders lower than that of conventional one.
We developed an optimized microchip-based fluorescence polarization immunoassay (FPIA) . Microchip-based FPIA was simple and rapid because it did not require several processes such as washing and reflowing and immobilization of antibodies or antigens in a channel. The quantitative analysis of theophylline was performed in short analysis time (65 seconds) with simple operation and reduced samples. The microchip-based FPIA will find a frequent use as point-of-care testing in clinical field. Furthermore we observed the behavior of fluorescence polarization after antigens and antibodies are mixed in a microchannel. The information will lead to the development of immunoassays using fluorescence polarization. Less
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Research Products
(81 results)
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[Presentation] Medical Diagnosis Using pTAS2007
Author(s)
Manabu, Tokeshi
Organizer
The 54th Annual Meeting of Japan Society of Laboratory Medicine, The 47th Annual Meeting of Japan Society of Clinical Chemistry "Impact of High-Tech Medicine Using New Technologies"
Place of Presentation
Osaka International Convention Center, Japan
Year and Date
2007-09-23
Description
「研究成果報告書概要(欧文)」より
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[Presentation] New Analytical Chemistry Using Microchips2006
Author(s)
Manabu, Tokeshi
Organizer
The Japan Society for Analytical Chemistry 55th Annual Meeting, Special Symposium "Trend of Separation Sciences"
Place of Presentation
Osaka University Toyonaka Campus, Japan
Year and Date
2006-09-20
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 次世代マイクロ診断システム2006
Author(s)
渡慶次学
Organizer
秋田県医工連携クラスター・第4回オープンセミナー
Place of Presentation
秋田市民交流プラザ
Year and Date
2006-01-27
Description
「研究成果報告書概要(和文)」より
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[Presentation] Next Generation Micro Diagnosis System2006
Author(s)
Manabu, Tokeshi
Organizer
Akita Cooperative Unit of Medicine and Engineering Research Cluster 4th Open Seminar
Place of Presentation
Akita Citizen Exchange Plaza, Japan
Year and Date
2006-01-27
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Microchip-Based Immunoassay2005
Author(s)
Manabu, Tokeshi
Organizer
Research Institute of Electrical Communication Tohoku University Kyodo Project Kenkyukai Nano Bioelectronics ni kansuru Kenkyu 4th Joho Biotronics Kenkyukai
Place of Presentation
Research Institute of Electrical Communication Tohoku University, Japan
Year and Date
2005-12-09
Description
「研究成果報告書概要(欧文)」より
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