2006 Fiscal Year Final Research Report Summary
Construction of a computational model for the transcriptional regulatory regions in Ciona intestinalis and its experimental verification
| Project/Area Number |
17310114
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NAKAI Kenta The University of Tokyo, Institute of Medical Science, Professor (60217643)
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| Co-Investigator(Kenkyū-buntansha) |
KUSAKABE Takehiro University of Hyogo, Graduate School of Life Science, Associate Professor (40280862)
YAMASHITA Riu University of Tokyo, Institute of Medical Science, Researcher (10401259)
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| Project Period (FY) |
2005 – 2006
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| Keywords | ascidians / promoters / Markov chain / model building / in situ hybridization / muscle-specific expression |
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| Research Abstract |
Transcriptional regulation is the first level of regulation of gene expression and is therefore a major topic in computational biology. For the studies of DNA regions that specify tissue-specific gene expression, we chose tunicates as our main model organisms and constructed a database of transcription in tunicates (esp. Ciona intestinalis): DBTGR (http: //dbtgr.hgc.jp/). Then, we introduced a Markov chain-based promoter structure model that uses both shared motifs and shared features from an input set of promoter sequences to predict candidate genes with similar expression. The model uses positional preference, order, and orientation of motifs. The trained model is used to score a genomic set of promoter sequences: high-scoring promoters are assumed to have a structure similar to the input sequences and are thus expected to drive similar expression patterns. We applied our model on two datasets in Caenorhabditis elegans and in Ciona intestinalis. Both computational and experimental verifications indicate that this model is capable of predicting candidate promoters driving similar expression patterns as the input-regulatory sequences. This model can be useful for finding promising candidate genes for wet-lab experiments and for increasing our understanding of transcriptional regulation
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Research Products
(8 results)
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[Journal Article] DBTGR:a database of tunicate promoters and their regulatory elements2006
Author(s)
Sierro, N., Kusakabe, T., Park, K.-J., Yamashita, R., Kinoshita, K., Nakai, K.
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Journal Title
Nucl.Acids Res. 34
Pages: D552-D555
Description
「研究成果報告書概要(和文)」より
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[Journal Article] DBTGR : a database of tunicate promoters and their regulatory elements.2006
Author(s)
Sierro, N., Kusakabe, T., Park, K.-J., Yamashita, R., Kinoshita, K., Nakai, K.
-
Journal Title
Nuci. Acids Res. 34
Pages: D552-D555
Description
「研究成果報告書概要(欧文)」より
